Hydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathways

Abstract

Ethnopharmacological relevanceCasearia sylvestris Sw. is widely used in popular medicine to treat conditions associated with pain. Aim of the studyThe present study investigated the influence of hydroalcoholic crude extract of Casearia sylvestris (HCE-CS) and contribution of pro-resolving mediators on mechanical hyperalgesia in a mouse model of chronic post-ischemia pain (CPIP). Methods and resultsMale Swiss mice were subjected to ischemia of the right hind paw (3 h ConclusionThese results reveal significant antihyperalgesic effect of HCE-CS on CPIP, mediated at least in part, by the pathway of resolution of inflammation centred on the axis modulated by ALX/FPR2.

Antihyperalgesic effect of exercise is augmented by the oral pretreatment with extract of Casearia sylvestris in an animal model of osteoarthritis

“…13 In particular, the analgesic effect of the plant was confirmed in different models of nociceptive, inflammatory, and immunological pain. 13,14…”

“…For the evaluation of mechanical hyperalgesia on days 5 and 6 after the beginning of the experiment, the mice were individually observed, as described earlier. 13 The hyperalgesic response was recorded as the frequency of paw withdrawal for 10 applications of the von Frey monofilament 0.6 g. Data were presented as a percentage of response to 10 stimulations. A significant increase in the frequency of response of animals after administration of CFA, relative to the basal values, was interpreted as a hyperalgesic response.…”

“…The mice belonging to the orally treated groups (ECE-CS or vehicle) were sent to the previously described procedures, while between days 5 and 7 animals received, once a day, the plant treatment immediately before swimming (days 5 and 6) or immediately after the measurement of the basal values of edema (day 7). As mentioned earlier, the dose of 30 mg/kg of ECE-CS was selected based on the previous study, 13 and 1 h after ECE-CS treatment, mice were evaluated for edema and mechanical hyperalgesia evaluations.…”

“…Despite the above mentioned, a possible benefit for a possible synergistic antihyperalgesic action from the association of physical exercise and oral treatment with CS in animal models of OA has not been observed yet. This possibility is mainly important with plants for which analgesic activity has already been observed, as for CS 13 and Citrus reticulata. 17 Thus, aiming at the importance of complementary therapies in the control of pain, it is possible to consider that the association between these two modalities of treatment promotes the benefit of obtaining an analgesic effect in chronic condition.…”

Anti-hyperalgesic properties of ethanolic crude extract from the peels of Citrus reticulata (Rutaceae)

“…Swiss mice were orally treated with sterile saline (0.1 ml/10 g weight) or different doses (30, 100 and 300 mg/kg) of ECE-CR, based on previous works performed in our laboratory in which this range of doses were antinociceptive for hydroalcoolic crude extraxt of other plant (De Mattos et al 2007, Piovezan et al 2017. One hour after this treatment, the animals received an intraplantar (i.pl.)…”