Located south of the West Bank, Wadi Al-Samen is considered one of the most important sources of groundwater recharge for the eastern aquifer in Hebron. It is polluted by sewage originating from domestic and industrial consumption in the Hebron area. Water quality assessment is an important criterion for achieving sustainable development. To evaluate water quality, twenty samples were collected from groundwater sources for two seasons and were analyzed for Physical properties (Total dissolved solids (TDS), Electrical conductivity (EC), potential hydrogen (pH), Temperature (T)), Four major cations (Mg 2+ , Ca 2+ , Na + and K + ), and the Major anions ( 3 HCO − , Cl − ,