Hydra constitutively expresses transcripts involved in vertebrate neural differentiation

Chatterjee, Sandipan; Lahudkar, Shweta; Godbole, N. N.; Ghaskadbi, Surendra

doi:10.1007/bf02703639

Cited by 6 publications

(7 citation statements)

References 18 publications

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“…Noggin transcripts, on the other hand, showed strong expression in the endoderm of hypostome, base of the tentacles, lower body column and in the basal region in whole polyps (Figure 3Bb, D). The observed expression of noggin in hypostome, base of the tentacles and foot region is comparable to our earlier observations made using Xenopus noggin probes in Hydra 37 . During budding, noggin continued to be expressed in the hypostome, base of the tentacles, lower body column and basal disc in the adult polyp (Figure 3Bc‐f) and at the sites where tentacles would emerge in the developing buds (shown by arrows in Figure 3Bc, d, e, 3D).…”

Section: Resultssupporting

confidence: 90%

“…The observed expression of noggin in hypostome, base of the tentacles and foot region is comparable to our earlier observations made using Xenopus noggin probes in Hydra. 37 During budding, noggin continued to be expressed in the hypostome, base of the tentacles, lower body column and basal In view of the expression of noggin in the hypostome and base of the tentacles in whole polyps and bud development, its localization in alsterpaullone treated polyps was examined, since this experimental condition induces formation of ectopic Organizers and tentacles in the body column of the polyps. 29 This happens as a consequence of upregulated Wnt signaling.…”

Section: Localization Of Gremlin and Noggin In Non-budding And Buddmentioning

confidence: 99%

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Differential expression of BMP inhibitors gremlin and noggin in Hydra suggests distinct roles during budding and patterning of tentacles

Krishnapati

Khade

Trimbake

et al. 2020

Developmental Dynamics

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Background: Mechanisms regulating BMP and Wnt pathways and their interactions are not well studied in Hydra. Results: We report identification of BMP inhibitor gremlin, comparison of its expression with that of noggin and possible antagonism between Wnt and BMP signaling in Hydra. Gremlin is expressed in body column with high levels in budding region and in early buds. Noggin, on the other hand, is expressed in the hypostome, base of tentacles, lower body column, and basal disc. During budding, noggin is expressed at the sites of tentacle emergence. This was confirmed in ectopic tentacles in polyps treated with alsterpaullone (ALP), a GSK-3β inhibitor that leads to upregulation of Wnt pathway. RT-PCR data show that upregulation of Wnt is accompanied by downregulation of bmp 5-8b though noggin and gremlin remain unaltered till 24 hours. Conclusions: Different expression patterns of gremlin and noggin suggest their roles in budding and patterning of tentacles, respectively. Further, bmp 5-8b inhibition by activated Wnt signaling does not directly involve noggin and gremlin in Hydra. Our data suggest that Wnt/BMP antagonism may have evolved early for defining the oral-aboral axis, while the involvement of BMP antagonists during axial patterning is a recent evolutionary acquisition within the Bilateria lineage.

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Section: Resultssupporting

confidence: 90%

Section: Localization Of Gremlin and Noggin In Non-budding And Buddmentioning

confidence: 99%

Differential expression of BMP inhibitors gremlin and noggin in Hydra suggests distinct roles during budding and patterning of tentacles

Krishnapati

Khade

Trimbake

et al. 2020

Developmental Dynamics

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“…3Bb). The observed expression of noggin in hypostome, base of the tentacles and foot region is comparable to our earlier observations made using Xenopus noggin probes in hydra (Chatterjee et al, 2001). During budding, noggin continued to be expressed in the hypostome, base of the tentacles, lower body column and basal disc in the adult polyp (Fig.…”

Section: Resultssupporting

confidence: 90%

Differential expression of BMP antagonists,gremlinandnogginin hydra: antagonism between Wnt and BMP pathways

Krishnapati

Khade

Trimbake

et al. 2019

Preprint

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13Mechanisms regulating BMP and Wnt signaling pathways have been widely studied in many 14 organisms. One of the mechanisms by which these pathways are regulated is by binding of 15 extracellular ligands. In the present study, we report studies with two BMP antagonists, gremlin 16 and noggin from Hydra vulgaris Ind-Pune and demonstrate antagonistic relationship between 17 BMP and Wnt pathways. Gremlin was ubiquitously expressed from the body column to head 18 region except in the basal disc and hypostome. During budding, gremlin was expressed 19 predominantly in the budding region suggesting a possible role in budding; this was confirmed in 20 polyps with different stages of buds. Noggin, on the other hand, was predominantly expressed in 21 the endoderm of hypostome, base of the tentacles, lower body column and at the basal disc in 22 whole polyps. During budding, noggin was expressed at the sites of emergence of tentacles 23 suggesting a role in tentacle formation. This was confirmed in alsterpaullone-treated polyps, 24 which showed noggin expression as distinct spots where ectopic organizers and ectopic tentacles 25 eventually formed. Using RT-PCR, we found that up-regulation of Wnt is accompanied with 26 down-regulation of BMP5-8b demonstrating antagonism between the two pathways. Down-27 regulation of noggin and gremlin, however, occurred only after 24 h recovery. The data suggest 28 that inhibition of BMP pathway by Wnt signaling in hydra does not directly involve noggin and 29 gremlin. Our findings indicate that the BMP/Noggin antagonism evolved early for setting up 30 2 and/or maintaining the head organizer while involvement of these BMP antagonists during 31 vertebrate axial patterning are recent evolutionary acquisitions. 32 33 Summary statement 36 We show that setting up of the Organizer by BMP/Noggin antagonism and role of BMP 37 inhibitors in tissue patterning are evolutionarily ancient, probably arising for the first time in 38 hydra 39 40 Introduction 41 Bone morphogenetic protein family, a subfamily of transforming growth factor-β (TGF-β) 42 superfamily was originally identified for role of some of its members in formation and 43 regeneration of bone in vivo. Subsequently, their roles in crucial developmental processes, such 44 as, proliferation, migration, differentiation and fate specification of embryonic cells, 45 morphogenesis and dorso-ventral patterning were discovered (Massagué, 1998; Komiya and 46 Habas, 2008). Non-optimal BMP signaling leads to a variety of developmental abnormalities and 47 disease conditions including cardiovascular diseases, symphalangism, diabetic nephropathy and 48 several types of cancers (Kattamuri et al., 2017). BMPs initiate downstream signaling by two 49 mechanisms. The first is a canonical smad-dependent pathway which involves phosphorylation 50 of Serine/Threonine kinase receptors, Type I and Type II receptor complexes (BMPRI and 51 BMPRII), and activation of smad-1/5/8. The second is a smad-independent pathway, which 52 involves TGF-β Activated Tyrosine Ki...

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“…The present study was undertaken to find out whether hydra has a noggin gene and whether it shows any structural and functional homology with vertebrate noggin in view of the earlier indirect evidence, using heterospecific ( Xenopus ) probes, that hydra expresses noggin ‐like transcripts (Chatterjee et al 2001). Hydra is an important model for evolutionary developmental biology since, in addition to a remarkable capacity of regeneration (reviewed in Bode 2003), tissue homeostasis in hydra is tightly regulated through continuous proliferation and differentiation of cells throughout the body column (Müller 1996).…”

Section: Introductionmentioning

confidence: 99%

Cloning of noggin gene from hydra and analysis of its functional conservation using Xenopus laevis embryos

Chandramore

Ito

Takahashi

et al. 2010

Evolution and Development

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Hydra, a member of phylum Cnidaria that arose early in evolution, is endowed with a defined axis, organized nervous system, and active behavior. It is a powerful model system for the elucidation of evolution of developmental mechanisms in animals. Here, we describe the identification and cloning of noggin-like gene from hydra. Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. Sequence analysis revealed that hydra Noggin shows considerable similarity with its orthologs at the amino acid level. When microinjected in the early Xenopus embryos, hydra noggin mRNA induced a secondary axis in 100% of the injected embryos, demonstrating functional conservation of hydra noggin in vertebrates. This was further confirmed by the partial rescue of Xenopus embryos by hydra noggin mRNA from UV-induced ventralization. By using animal cap assay in Xenopus embryos, we demonstrate that these effects of hydra noggin in Xenopus embryos are because of inhibition of BMP signaling by Noggin. Our data indicate that BMP/Noggin antagonism predates the bilaterian divergence and is conserved during the evolution.

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Hydra constitutively expresses transcripts involved in vertebrate neural differentiation

Cited by 6 publications

References 18 publications

Differential expression of BMP inhibitors gremlin and noggin in Hydra suggests distinct roles during budding and patterning of tentacles

Differential expression of BMP inhibitors gremlin and noggin in Hydra suggests distinct roles during budding and patterning of tentacles

Differential expression of BMP antagonists,gremlinandnogginin hydra: antagonism between Wnt and BMP pathways

Cloning of noggin gene from hydra and analysis of its functional conservation using Xenopus laevis embryos

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