Differential expression of <scp>BMP</scp> inhibitors gremlin and noggin in Hydra suggests distinct roles during budding and patterning of tentacles

Krishnapati, Lakshmi Surekha; Khade, Samiksha; Trimbake, Diptee; Patwardhan, Rohan; Nadimpalli, Siva Kumar; Ghaskadbi, Surendra

doi:10.1002/dvdy.238

Cited by 5 publications

(6 citation statements)

References 54 publications

(159 reference statements)

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“…Half of the genes involved in mitochondrial biosynthesis were also up-regulated at branch tips. Further, two different bone morphogenetic proteins (BMPs), which may play a role in axial patterning in cnidarian polyps (Reinhardt et al, 2004) and in budding (Surekha et al, 2020), were up-regulated at branch tips here. Finally, several retinol dehydrogenases, proteins associated with photoreceptors in higher organisms (Albalat, 2012), were up-regulated at branch tips, where light levels tend to be higher, an observation shared with Acropora spp.…”

Section: Developmental Pathways Are De Along the Branch Axismentioning

confidence: 70%

Physiological and Transcriptomic Variability Indicative of Differences in Key Functions Within a Single Coral Colony

et al. 2021

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Polyps in different locations on individual stony coral colonies experience variation in numerous environmental conditions including flow and light, potentially leading to transcriptional and physiological differences across the colony. Here, we describe high-resolution tissue and skeleton measurements and differential gene expression from multiple locations within a single colony of Stylophora pistillata, aiming to relate these to environmental gradients across the coral colony. We observed broad transcriptional responses in both the host and photosymbiont in response to height above the substrate, cardinal direction, and, most strongly, location along the branch axis. Specifically, several key physiological processes in the host appear more active toward branch tips including several metabolic pathways, toxin production for prey capture or defense, and biomolecular mechanisms of biomineralization. Further, the increase in gene expression related to these processes toward branch tips is conserved between S. pistillata and Acropora spp. The photosymbiont appears to respond transcriptionally to relative light intensity along the branch and due to cardinal direction. These differential responses were observed across the colony despite its genetic homogeneity and likely inter-polyp communication. While not a classical division of labor, each part of the colony appears to have distinct functional roles related to polyps’ differential exposure to environmental conditions.

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Section: Developmental Pathways Are De Along the Branch Axismentioning

confidence: 70%

Physiological and Transcriptomic Variability Indicative of Differences in Key Functions Within a Single Coral Colony

et al. 2021

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“…Moreover, recent studies have uncovered the synergistic relationship between Noggin and the neural differentiation of BMSCs, with Noggin effectively inhibiting the osteogenic effects of BMP2/4, thereby potentially facilitating the development of the nervous system [17][18][19][20]. In light of these findings, our study was designed to leverage Noggin in inducing BMSCs into neurons and investigate their consequential roles in promoting recovery in a rat model of SCI.…”

Section: Discussionmentioning

confidence: 99%

Noggin Protein can Induce the Differentiation of Rat Bone Marrow Mesenchymal Stem Cells to Neurons and Repair Spinal Cord Injury

Liu,

Luo,

et al. 2023

Discovery Medicine

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Background: Addressing spinal cord injury (SCI) through stem cell therapy is currently at the forefront of medical research despite its complexity. In this study, we investigated the potential of the Noggin protein in promoting the differentiation of rat bone marrow mesenchymal stem cells (BMSCs) into neuronal cells. We transplanted induced cells into a rat model with spinal cord injury. This exploration proposes an innovative perspective on stem cell therapies for spinal cord injuries. Methods: Rat BMSCs were isolated utilizing the bone marrow cell apposition method; The multidirectional differentiation of rat BMSCs was identified by lipid induction and osteogenic induction; Rat BMSCs were induced by different concentrations of Noggin protein and different induction times; Nissel staining was used to identify the induced neuronal-like cells; The expression of synaptic protein Ⅰ (SYN1), glial fibrillary acidic protein (GFAP), and neurofilament protein 200 (NF200) in neuron-like cells was detected by immunofluorescence assay. Rats were randomly divided into a control group and a neuron-like cell group; A rat spinal cord injury model was produced, and neuron-like cells obtained from induction were transplanted into the rat's SCI. The recovery of the rats' hind limbs' motor function was detected by the Basso, Beattie, and Bresnahan (BBB) scores, and the changes in the expression of NF200 mRNA at the spinal cord injury were detected by quantitative real time polymerase chain reaction (qRT-PCR). Results: Our cultured rat BMSCs had a long spindle-shaped morphology and stained positively for oil red O after lipogenic induction and modified alizarin red S after osteogenic induction. Nissel staining of cells obtained from rat BMSCs induced by Noggin protein was positive. Immunofluorescence results showed that the induced neuronal-like cells positively expressed NF200 and SYN1, and negatively expressed GFAP. After local transplantation of induced neuronal-like cells in the rat SCI model, the BBB scores in the neuron-like cell group were higher than those in the control group at 1 w, 2 w, and 4 w, with statistically different results (p < 0.05). According to qRT-PCR results, NF200 at the spinal cord injury in the neuron-like cell group was higher than that in the control group at 12 h, 3 d, 1 w, 2 w, and 4 w, with statistically significant differences in results (p < 0.05). Conclusions: Our findings indicate that Noggin protein effectively facilitates the differentiation of rat BMSCs into neuronal cells, highlighting its potential as a therapeutic agent for repairing spinal cord injuries. This study elucidates a promising avenue in stem cell research, contributing a novel approach to regenerative strategies for spinal cord injuries.

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“…One of the important mechanisms of bmp pathway regulation is by binding of secretory molecules to bmp ligands, which prevent them from interacting with their cell surface receptors. so far, chordin, noggin and gremlin have been identified from Hydra and are shown to have role in axial and tentacle patterning (rentzsch et al, 2007;Chandramore et al, 2010;krishnapati et al, 2020). Identification of noggin in Hydra came from our own studies.…”

Section: Nogginmentioning

confidence: 97%

“…structurally, Hydra noggin shows characteristic Cysteine ring motif as seen in human noggin that provides conformational rigidity to the protein (Groppe et al, 2002;krishnapati et al, 2020). although structural conservation does not fully imply functional conservation in higher metazoans, injection of Hydra noggin mrna in two cell stage Xenopus embryos resulted in partial duplication of axis in 100 % embryos.…”

Section: Nogginmentioning

confidence: 99%

“…Furthermore, Wnt has been shown to act directly on different components of TGF-β pathway during secondary axis patterning in Hydra. Work from our laboratory has shown the roles of two bmp antagonists noggin and Gremlin in axis formation and tentacle patterning in Hydra (Chandramore et al, 2010;krishnapati et al, 2020). Over the past few years, quite a few players from the TGF-β pathway have been identified in Hydra and have been shown to play key roles in axial patterning (Table 1).…”

Section: Axial Patterning In Hydramentioning

confidence: 99%

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TGF-β signaling molecules in Hydra: role of BMP and BMP inhibitors during pattern formation

Krishnapati,

Ghaskadbi

2024

Int. J. Dev. Biol.

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Understanding the evolution of body plans has been one of the major areas of investigation in developmental and evolutionary biology. Cnidaria, the sister group to bilaterians, provides an opportunity to elucidate the origin and evolution of body axes. Hydra, a freshwater cnidarian, is a useful model to study signaling pathways governing pattern formation, which are conserved up to vertebrates including humans. The transforming growth factor β (TGF-β) signaling pathway is one of the fundamental pathways that regulate axis formation and organogenesis during embryonic development. In this article, we discuss the TGF-β pathway members identified in Hydra along with other cnidarians with an emphasis on bone morphogenetic proteins (BMPs) and their inhibitors. TGF-β members, especially those involved in BMP signaling pathway, are mainly involved in maintaining the Organizer region and patterning the body axis in Hydra. Identification of other members of this pathway in Hydra and fellow cnidarians would provide insights into the evolution of body axes and pattern formation in more complex metazoans.

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Differential expression of BMP inhibitors gremlin and noggin in Hydra suggests distinct roles during budding and patterning of tentacles

Cited by 5 publications

References 54 publications

Physiological and Transcriptomic Variability Indicative of Differences in Key Functions Within a Single Coral Colony

Physiological and Transcriptomic Variability Indicative of Differences in Key Functions Within a Single Coral Colony

Noggin Protein can Induce the Differentiation of Rat Bone Marrow Mesenchymal Stem Cells to Neurons and Repair Spinal Cord Injury

TGF-β signaling molecules in Hydra: role of BMP and BMP inhibitors during pattern formation

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