Hybrids of Passiflora: P. gardneri versus P. gibertii, confirmation of paternity, morphological and cytogenetic characterization

Belo, Gabriela de Oliveira; Souza, Margarete Magalhães; Silva, Gonçalo Santos; Lavinscky, Manuella Palmeira

doi:10.1007/s10681-017-2021-2

Cited by 11 publications

(5 citation statements)

References 31 publications

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“…foetida using microsatellite markers. Belo et al (2018) also confirmed interspecific hybridization between P. gardneri and P. gibertii using the SSR Pe75, a marker that amplified heterozygous bands of 300 and 350 pb.…”

Section: Confirmation Of Hybridization By Molecular Markersupporting

confidence: 58%

Genetic resistance to fusariosis and base rot in interspecific F1 progenies of passion fruit (Passiflora spp.)

Marostega

Preisigke

Chimello

et al. 2020

RSD

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Fusariosis and base rot, caused by the fungi Fusarium oxysporum f. sp. passiflorae and F. solani, respectively, seriously damage the cultivation of sour passion fruit in Brazil. This work aims to obtain and evaluate F1 hybrids of Passiflora spp. wild plants resistant to fusariosis and base rot using genotypes of P. edulis (commercial species) and certify interspecific hybridizations using microsatellite markers. Hybridizations were performed using a P. edulis female parent and P. nitida and P. mucronata male parents for crosses aiming fusariosis resistance, and P. nitida, P. cincinnata and P. quadrangularis for hybridizations aiming tolerance to base rot. 35 microsatellite markers were used to confirm hybridization. The washed roots method was used for fusarium resistance tests and inoculation procedure with a mycelium disk fixed on a small wound on the plant stem for base rot. The interspecific hybridizations provided 49 potentially hybrid genotypes. Confirmation of hybridization by microsatellite marker was verified for 57% of the analyzed genotypes. The hybrids 115-1, 115-3, 115-4, 115-5, 115-6, 115-7, 115-9 and 128 are indicated as promising genotypes for a new stage of the breeding program. In the resistance evaluation of 13 F1 hybrids to F. oxysporum f. sp. passiflorae, the genotypes 142 and 143-2 were selected as the most resistant to continue the breeding program.

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Section: Confirmation Of Hybridization By Molecular Markersupporting

confidence: 58%

Genetic resistance to fusariosis and base rot in interspecific F1 progenies of passion fruit (Passiflora spp.)

Marostega

Preisigke

Chimello

et al. 2020

RSD

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“…Estudos como este que avaliam a transferibilidade de primers microssatélites em Passifloracea tornam-se extremamente úteis, pois são ferramentas que antecedem estudos na área da genética vegetal, possibilitando medir os efeitos evolutivos e de seleção natural das espécies, e realizar análises filogenéticas, construções de mapas gênicos, análise de diversidade genética, e estudos de preservação da população durante programas de melhoramento genético (Gonçalves-Vidigal & Rubiano, 2011;Pereira et al, 2015). Além de serem capazes de acompanhar relações intra e interespécies (Araya et al, 2017), e serem utilizados na confirmação de paternidade de híbridos (Belo et al, 2018), otimizando assim os estudos de caracterização molecular das espécies silvestres de maracujazeiro, uma vez que através da análise de transferibilidade é possível otimizar os custos onerosos e longos períodos de desenvolvimento de novos marcadores SSR.…”

Section: Resultsunclassified

Transferibilidade de marcadores microssatélites para espécies silvestres de maracujazeiro

Silva

Marostega

Gílio

et al. 2019

RCA

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Marcadores moleculares permitem acompanhar o progresso de um programa de melhoramento genético. Marcadores SSR (simple sequence repeats), por serem codominantes, multialélicos e apresentarem alto polimorfismo, são ideais para estudos de criação de mapas gênicos, variabilidade e confirmação de retrocruzamentos. Porém, o uso deste tipo de marcador molecular é limitado, visto que são apenas disponíveis primers para espécies com expressão comercial. Neste contexto, o objetivo deste trabalho foi avaliar a transferibilidade de 21 loci microssatélites desenvolvidos e desenhados de P. edulis e P. alata para quatro espécies silvestres de Passiflora. As espécies utilizadas foram: P. cincinnata, P. quadrangularis, P. mucronata, e P. nitida. A extração de DNA foi realizada através do Kit Promega Wizard®. O protocolo de amplificação utilizado foi adaptado do proposto para o maracujazeiro comercial. Foram utilizados dezenove marcadores microssatélites desenhados para a espécie P. edulis e dois desenhados para P. alata. Os amplicons foram levados à eletroforese horizontal em gel de agarose a 3% e corados com o buffer Nancy 520. Os géis foram revelados em fotodocumentador digital e analisados. A transferibilidade foi verificada através da avaliação de presença ou ausência de banda. Os loci microssatélites projetados para P. edulis e P. alata mostraram-se transferíveis para ao menos uma das espécies com uma taxa altamente variável sendo, P. cincinnata (33,33%), P. quadrangularis (23,81%), P. mucronata (19,05%) e P. nitida (14,28%). Os marcadores PE13 e PE54 podem ser indicados para estudos de caracterização molecular por serem os únicos capazes de amplificar todas as espécies de maracujá silvestre estudadas.

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“…Most Passiflora species are allogamous, diploid, with 2n = 12, 18, or 20 chromosomes (Yotoko et al, 2011), with ornamental potential and medicinal properties (Belo et al, 2018;He et al, 2020), due to its contents of alkaloids, flavonoids and carotenoids, minerals and vitamins A, C, and D. The seeds contain essential fatty acids (55-66% linoleic acid, 18-20% oleic acid, and 10-14% palmitic acid), that can be used in the food and cosmetic industries (da Silva et al, 2015).…”

Section: Issr Amplificationmentioning

confidence: 99%

Molecular characterization of Passiflora edulis f. flavicarpa Degener with ISSRs markers

Morillo,

Muñoz,

Morillo

2023

Braz. J. Biol.

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Passiflora edulis it is a specie widely distributed and cultivated in Colombia, with economic potential. Although there is a wide genetic and phenotypic variability, it has not yet been explored through the use of molecular techniques. This study aimed to characterize the structure and genetic diversity of P. edulis cultivars using ISSR markers. The study was carried out using leaf samples from 21 cultivars of P. edulis collected within a productive system in the department of Boyacá, Colombia, using seven ISSR primers. Genetic similarity was used to cluster by the UPGMA method, polymorphic information content (PIC), expected heterozygosity (He), Shannon index (I), gene flow (Nm), and coefficient of genetic differentiation (Gst) were estimated using POPGENE and TFPGA software. The Bayesian model and analysis of molecular variance (AMOVA) were used to assess the genetic structure. Cultivars of P. edulis showed high polymorphism rates. Seven ISSR produced 138 loci. The cluster analysis formed two groups according to the genetic similarity and phenotypic characteristics associated mainly with the fruit. The average value of expected heterozygosity was 0.29 for the total population and 0.27 and 0.22 for groups I and II, respectively. AMOVA indicates higher diversity within groups, but not between groups showing levels of hierarchy different from those considered in this study. Moderate genetic differentiation (Gst=0.12) and high gene flow (Nm=3.91) are observed.

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Hybrids of Passiflora: P. gardneri versus P. gibertii, confirmation of paternity, morphological and cytogenetic characterization

Cited by 11 publications

References 31 publications

Genetic resistance to fusariosis and base rot in interspecific F1 progenies of passion fruit (Passiflora spp.)

Genetic resistance to fusariosis and base rot in interspecific F1 progenies of passion fruit (Passiflora spp.)

Transferibilidade de marcadores microssatélites para espécies silvestres de maracujazeiro

Molecular characterization of Passiflora edulis f. flavicarpa Degener with ISSRs markers

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