Hybridoma cell line production of a specific monoclonal antibody to the mycotoxins zearalenone and .alpha.-zearalenol

Dixon, D.; Warner, Roscoe L.; Ram, B. P.; Hart, L. P.; Pestka, James J.

doi:10.1021/jf00073a026

Cited by 45 publications

(21 citation statements)

References 29 publications

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“…Recovery values slightly higher than 100% are obtained, in a remarkable agreement with those obtained by competitive direct monoclonal ELISA [31], ELISA with scFvQY1.5 (being scFvQY1.5 a specific recombinant antibody for ZEA) [6] and pressurized liquid extraction/LC-MS [8] on recovery of ZEA also from spiked corn. Reasons for these findings are not clear yet.…”

Section: Zea From Maize Samplessupporting

confidence: 72%

Development of an Electroanalytical Method for the Quantification of Zearalenone (ZEA) in Maize Samples

et al. 2005

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The application of electroanalytical techniques to detect and quantify zearalenone (ZEA) mycotoxin that frequently contaminates maize and foodstuff is studied in this work. Rice and maize grains were inoculated with Fusarium fungus to obtain ZEA in artificially infected samples. The electro-oxidation of ZEA adsorbed on the surface of glassy carbon (GC) electrodes in 20% acetonitrile (ACN) þ 80% 1 M HClO 4 (aqueous solution) reaction medium was studied by using square-wave voltammetry (SWV). Studies were conducted to find the most favorable accumulation potential (E acc ) and accumulation time (t acc ) to perform the ZEA preconcentration on the electrode surface. It was found that E acc was any value in the range from 0.00 -0.90 V and the best t acc was 120 s, respectively, for ZEA separated from extracting solution by TLC (ZEA TLC ) while E acc ¼ 0.90 V corresponded to ZEA in non separated matrix solution (ZEA matrix ). The ZEA quantitative determination was performed by SWV combined with the standard addition method. Linear plots were obtained from the net peak current (I p, n ) vs c* ZEA in the concentration range from 20 to 3184 ppb. Detection limit of 30 ppb at a signal to noise ratio of 3 : 1 was obtained. On the other hand, recovery experiments were performed on uncontaminated maize samples spiked with ZEA.

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Section: Zea From Maize Samplessupporting

confidence: 72%

Development of an Electroanalytical Method for the Quantification of Zearalenone (ZEA) in Maize Samples

et al. 2005

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“…Immunization was performed according to a slight modification of methods described previously [22,23]. Adult BALB/c mice (male) were immunized by subcutaneous injection with FB 1 -KLH (50.0 mg).…”

Section: Production Of Monoclonal Antibody (Mab)mentioning

confidence: 99%

“…Measurement of cross-reactivity FB 1 and some related compounds were measured for crossreactivity by indirect competitive ELISA (IDC-ELISA) according to the previously described method [23]. The molar FB 1 concentration that resulted in 50 % inhibition (IC 50 ) was used to calculate cross-reactivity using the following equation: % cross-reactivity = [IC 50 (FB 1 )/IC 50 (cross -reactivity compound)] × 100…”

Section: Production Of Monoclonal Antibody (Mab)mentioning

confidence: 99%

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Urchin-like gold nanoparticle-based immunochromatographic strip test for rapid detection of fumonisin B1 in grains

Ren

Huang

et al. 2015

Anal Bioanal Chem

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An immunochromatographic strip (ICS) using urchin-like gold nanoparticles (UGNs) for sensitive detection of fumonisin B1 (FB1) was developed to meet the requirement for rapidly monitoring FB1 in grain samples. The sensitivity of the ICS was 5.0 ng/mL, which represents a fourfold increase in sensitivity over conventional strip preparation using colloidal gold as the antibody-labeled probe. Analysis of FB1 in grain samples showed that data obtained from the strip tests were in a good agreement with those obtained from HPLC and enzyme-linked immunosorbent assays (ELISAs). This qualitative test did not require any specialized equipment, and the detection time was less than 5 min, which is suitable for on-site testing of FB1 in grain samples. Overall, to our knowledge, this is the first report of using a UGN as the antibody-labeled probe for sensitive detection of FB1 in grains using an ICS. Graphical Abstract Preparation of ICS using conventional colloidal gold and urchin-like gold nanoparticle, respectively.

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“…The detailed procedure is described in Figure 1. The procedure for the MAb production and immunoglobulin preparation were performed as described previously (Dixon, Warner, Ram, Hart, & Pestka, 1987;Nakazawa, Mukumoto, & Miyatake, 2010). Briefly, 10 female BALB/c mice were first immuned subcutaneously with 3-HS-DON-BSA in Freund's complete adjuvant.…”

Section: Mab Productionmentioning

confidence: 99%

Development of a rapid competitive indirect ELISA procedure for the determination of deoxynivalenol in cereals

Shi

Shen

et al. 2012

Food and Agricultural Immunology

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A rapid and sensitive method based on competitive indirect enzyme-linked immunosorbent assay (ciELISA) was developed and validated for the detection of DON (deoxynivalenol) in cereals with the confirmation of the reliable ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS). Within this method, the IC50 (half maximal inhibitory concentration of a substance) of the DON-specific monoclonal antibody (MAb) we produced was 61.10 ng/mL. The limit of detection (LOD) value, measured by IC10, was 6.12 ng/ mL, which is below the maximum residue levels (MRLs) established for DON in various cereals. In spiked samples, the recoveries ranged from 79.3% to 115.2% for maize and from 74.3% to 118.3% for wheat. This method was compared with the UPLC-MS/MS method by testing four concentrations. The correlation coefficient for the two methods was 0.9884 in a linear-regression analysis. The results illustrated the reliability of the ciELISA method for the determination of cereal samples.

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Hybridoma cell line production of a specific monoclonal antibody to the mycotoxins zearalenone and .alpha.-zearalenol

Cited by 45 publications

References 29 publications

Development of an Electroanalytical Method for the Quantification of Zearalenone (ZEA) in Maize Samples

Development of an Electroanalytical Method for the Quantification of Zearalenone (ZEA) in Maize Samples

Urchin-like gold nanoparticle-based immunochromatographic strip test for rapid detection of fumonisin B1 in grains

Development of a rapid competitive indirect ELISA procedure for the determination of deoxynivalenol in cereals

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