Hybridization Technique for Forest Trees

Abstract

The relative merits of different materials as coverings to protect receptive female flowers against chance pollination were studied, and a combination of glassine (inner) and kraft (outer) bags was adopted. Pollination was effected as follows: the kraft bag was removed and the glassine bag punctured with the point of a specially designed pollen gun; the pollen was then driven into the bag by squeezing the bulb of the gun; finally, the puncture was sealed with an adhesive patch and the kraft bag replaced. The pollen gun attaches to pollen containers (small Erlenmeyer flasks) that can be stored over calcium chloride.

Hybridization and Natural Gene‐flow Between Higher Plants

“…Bradshaw at Bangor. Johnson (1946a, b;Johnson & Bradley, 1946;Johnson & Heimburger, 1946) has shown the way in the artificial hybridization of many forest trees.…”

Inbreeding Experiments With the Douglas Fir

“…~h k cones soon matured at room teAperature and the pillen was collected and tested for viability before being stored at 3 4 O F. until required. When the female conelets had fully emerged from the bud scales, they were artificially pollinated by means of the pollen gun developed by Johnson (7). The pollen was blown through a small hole in the viscose casing which was then sealed with cellulose tape.…”