Hybridization of
            <i>Salmonella</i>
            Species by Mating with
            <i>Escherichia coli</i>

Baron, L. S.; Spilman, W. M.; Carey, Warren F.

doi:10.1126/science.130.3375.566

Cited by 35 publications

(20 citation statements)

References 7 publications

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“…Lac + progeny possessing the unchanged antigenic pattern of the female typhoid parent was detected at low frequency with a number of the S. typhosa strains tested. One of the Lac + hybrids of S. typkosa, strain 643, which will be referred to as 643L + was selected for further use in mating experiments (6). The availability of a number of selective markers in this strain as well as its nutritional requirements made it especially useful for genetic studies.…”

Section: Resultsmentioning

confidence: 99%

“…These matings were plated on media with different selective substrates and the recombination frequencies were determined. Previous results (6) indicated that the frequency of recombination (selection for Lac + hybrids) using the Lac-F-S. typhosa strain 643L-was approximately 1 X 10-4. In the present experiments, selection for L-Ara + hybrids gave a frequency of 1 X 10 -~ recombinants which is about 10 per cent of the total number of recombinants when selection is made for Lac + hybrids.…”

Section: Discussionmentioning

confidence: 99%

“…These studies dealt with the isolation of a streptomycin-resistant m u t a n t of S. typhimurium which acted as a high frequency recipient strain in matings with E. coli. Further studies b y Baron et al (6) have established the ability of other Salmonella species to cross with E. coli. This communication is concerned with an analysis of hybrids from crosses between E. coli and Salmonella typhosa, in which the S. typhosa strains behave as recipient organisms for genetic material transferred from E. coli cells.…”

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confidence: 99%

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Diploid Heterozygous Hybrids From Matings Between Escherichia Coli and Salmonella Typhosa

Baron

Spilman

Carey

1960

The Journal of Experimental Medicine

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Sexuality in bacteria (1), now considered to be a unilateral conjugation (2, 3), has been studied in Escherichia coli b y m a n y workers. Luria and Burrous (4) have extended this form of genetic recombination to bacteria classified in different genera with their description of mating between E. coli and Shigella species. Recently, successful crosses between E. coli and Salmonella typhimurium were reported b y Baron eta/. (5). These studies dealt with the isolation of a streptomycin-resistant m u t a n t of S. typhimurium which acted as a high frequency recipient strain in matings with E. coli. Further studies b y Baron et al. (6) Materials and MethodsCultures.--The strains of S. typhosa employed were obtained from the culture collection at the Waiter Reed Army Institute of Research. A high frequency of recombination (Hfr) derivative of E. ¢otl K-12, strain W-1895, was obtained from Dr. P. D. Skaar, and a D-arabinose utilizing (v-Ara) mutant of this organism was isolated and employed in these studies.Media.--Meat extract agar, minimal media, EMB agar, and other culture media were used as described previously (5).Antisera.--Rabbit antisera were prepared against E. coU, S. typhosa, and selected hybrids by a series of intravenous injections of 109 viable or heat-killed (100°C. for 2 hours) cells. Absorbed sera were prepared as described by Edwards and Ewing (7).Phages.--The Vi typing phages were obtained from Dr. Cora Gunther; the procedures used for propagating these phages have been described by Baron et aL (8). The T series of bacteriophages were received from Dr. G. Bertani.Recombination Procedures.--For the detection of strains fertile with E. coli K-12, Hfr strain W-1895, the various cultures of S. typhosa which were to be tested as recipients (females) were spread with the E. coli culture as the donor (male) strain at a ratio of twenty recipient cells to one donor cell on an appropriate selective medium. The E. coli culture was grown in

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Diploid Heterozygous Hybrids From Matings Between Escherichia Coli and Salmonella Typhosa

Baron

Spilman

Carey

1960

The Journal of Experimental Medicine

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“…Finally, although attempted crosses of Escherichin coli with many Snlr7zorzelln types and of types of Sul~nonelln with one another have usually failed, Baron has demonstrated crosses of Etcherichin coli with a unique strain of Sc~lnzonellcl typhimrtrium (3). This-may be especially useful as a means of developing hybrids which can be used to bridge the studies of sexuality in Escherichin coli and transduction in Snln7onella.…”

Section: Genetic Recombination In Bacteriamentioning

confidence: 99%

A View of Genetics

Lederberg

1960

Science

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“…Strain ST-2 was found to react as a recipient (phenotypically F-) strain in these experiments with frequencies similar to those reported for other F-S. typhosa previously studied."' 14 Thus, in this manner, it was possible to obtain hybrids of ST-2 which are able to utilize 1-arabinose, and/or 1-rhamnose, and which can produce indol. These hybrids still retained their ability to mate as males with the cultures listed in Table 1.…”

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