Hybrid wide-field and scanning microscopy for high-speed 3D imaging

Duan, Yubo; Chen, Nanguang

doi:10.1364/ol.40.005251

Cited by 5 publications

(2 citation statements)

References 18 publications

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“…A similar hybrid imaging strategy, which combines confocal microscopy with wide-field imaging, has been proved advantageous in our previous study [25]. The choice of cutoff frequency is rather empirical at the moment.…”

Section: Introductionmentioning

confidence: 99%

Augmented line-scan focal modulation microscopy for multi-dimensional imaging of zebrafish heart in vivo

Pant¹,

Duan²,

Xiong³

et al. 2017

Biomed. Opt. Express

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Multi-dimensional fluorescence imaging of live animal models demands strong optical sectioning, high spatial resolution, fast image acquisition, and minimal photobleaching. While conventional laser scanning microscopes are capable of deep penetration and sub-cellular resolution, they are generally too slow and causing excessive photobleaching for volumetric or time-lapse imaging. We demonstrate the performance of an augmented line-scan focal modulation microscope (aLSFMM), a high-speed imaging platform that affords above video-rate imaging speed by the use of line scanning. Exceptional background rejection is accomplished by combining a confocal slit with focal modulation. The image quality is further improved by merging the information from simultaneously acquired focal modulation and confocal images. Such a hybrid imaging scheme makes it possible to use very low power excitation light in high-speed imaging, and therefore leads to reduced photobleaching that is desirable for three-dimensional (3D) and four-dimensional (4D) in vivo image acquisition.

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Section: Introductionmentioning

confidence: 99%

Augmented line-scan focal modulation microscopy for multi-dimensional imaging of zebrafish heart in vivo

Pant¹,

Duan²,

Xiong³

et al. 2017

Biomed. Opt. Express

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“…20 For the monitoring of these fast and dynamics processes without causing detrimental effects by observation, video rate image acquisition becomes necessary. 21,22 Here, we report a line-scan FMM (LSFMM) system that addresses the above issues. LSFMM speeds up the image acquisition process by scanning a line focus in one-dimension (1-D) only.…”

mentioning

confidence: 99%

Line-scan focal modulation microscopy

Pant

Gong

et al. 2017

J. Biomed. Opt.

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Polarization-resolved hyperspectral stimulated Raman scattering microscopy for label-free biomolecular imaging of the tooth

Wang

Zheng

Hsu

et al. 2016

Applied Physics Letters

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We report the development and implementation of a rapid polarization-resolved hyperspectral stimulated Raman scattering (SRS) microscopy technique for label-free biomolecular imaging of the tooth. The hyperspectral SRS imaging technique developed covers both fingerprint (800–1800 cm−1) and high-wavenumber (2800–3600 cm−1) regions for tooth Raman imaging without fluorescence background interference with an imaging speed of <0.3 s per frame of 512 × 512 pixels (∼1 μs per pixel), that is, >106 faster than confocal Raman imaging. Significant differences of hyperspectral SRS spectra among different tooth locations (e.g., dentin, enamel, and dentin-enamel junction) are observed, revealing the biochemical distribution differences across the tooth. Further polarization-resolved SRS imaging shows different polarization dependences related to the molecular orientation differences of various tooth locations. This work demonstrates the potential of polarization-resolved hyperspectral SRS imaging technique developed in rapidly characterizing biochemical structures and compositions as well as biomolecule organizations/orientations of the tooth without labeling.

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Hybrid wide-field and scanning microscopy for high-speed 3D imaging

Cited by 5 publications

References 18 publications

Augmented line-scan focal modulation microscopy for multi-dimensional imaging of zebrafish heart in vivo

Augmented line-scan focal modulation microscopy for multi-dimensional imaging of zebrafish heart in vivo

Line-scan focal modulation microscopy

Polarization-resolved hyperspectral stimulated Raman scattering microscopy for label-free biomolecular imaging of the tooth

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