Hybrid Oxygen-Responsive Reflective Bragg Grating Platforms

Abstract

Oxygen responsive sensor platforms were fabricated by pin printing tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) ([Ru(dpp)3]2+) doped sols onto wavelength tuned reflective Bragg gratings. In an epi-luminescence configuration, these Bragg gratings (Gr) were designed to reflect selectively the O2 responsive [Ru(dpp)3]2+ emission toward the detector to enhance the detected signal magnitude. The xerogel based sensors were formed onto: (i) glass (XGl), (ii) directly on top of the grating (XGrGl), or (iii) on the glass substrate opposite the grating (XGlGr). The results show that all sensors exhibit linear, statistically equivalent O2 sensitivities and the XGrGl platform yields up to an 8 fold increase in relative detected analytical signal (RDAS) in comparison to the control (XGl) platform.

High-Throughput Screening System for Creating and Assessing Surface-Modified Porous Silicon

“…In previous research from our laboratories on pin-printed xerogel microarrays formed on glass 21,–2428 we sometimes form features that are not uniform. However, this spatial heterogeneity had no detectable impact on the analytical performance in our previous platforms where the active agents were sequestered directly within the xerogel.…”

“…Pin-printing strategies in concert with PL and IR imaging can be used for high-throughput screening on pSi to identify chemistries that alter the pSi in favorable ways. However, unlike previous high-throughput pin-printing methods, 21,–2428 one must exercise some caution with pSi to ensure that the pin-printed coatings lack defects where agents might be able to directly access the pSi and, from under the coating, destroy the fragile pSi network.…”

Spatial Characteristics of Contact Pin-Printed Silanes and Bioconjugates on Oxidized Porous Silicon

“…The APTES- and BATES-ox-pSi microarrays were assessed by PL and FT-IR spectroscopy. PL measurements were performed by using an epi-luminescence multispectral imaging microscope . The PL-based APTES- and BATES-derived spot intensity profiles were constructed by using the ImageJ Radial Profile plugin.…”

Contact Pin-Printing onto Porous Silicon for Creating Microarrays with High Chemical Diversity

“…Photoluminescence was measured using a wide-field epi-luminescence multispectral imaging system, 12 consisting of a 325 nm He-Cd laser (Omnichrome) and an epi-luminescence microscope (BX-40, Olympus) with a 4 × microscope objective (Olympus). The microscope filter cube contained a 325 nm bandpass filter (325BP15, Omega Optics) to clean up the excitation beam, a dichroic long-pass filter (410 nm) (410DRLP, Omega Optics), and a 410 nm long-pass emission filter (410EFLP, Omega Optics) to minimize Rayleigh scatter from reaching the detection system.…”

“…Contact pin-printing is central to several important technologies. [1][2][3][4][5][6][7][8][9][10][11][12][13] For example, contact pin-printing is used to: (i) create high-density DNA or protein microarrays, 1,5,6,9,14,15 (ii) effect high-throughput materials screening campaigns, 2,4,7,11 and (iii) develop chemical sensor arrays. 3,8,13 Thus, because each pin-printed feature (spot) is spatially isolated from its neighbors, contact pinprinting allows one to create, evaluate, and exploit diverse pageants of chemistries simultaneously in a small footprint.…”