Hybrid Mass Spectrometry: Towards Characterization of Protein Conformational States

A Mass‐Spectrometry‐Based Modelling Workflow for Accurate Prediction of IgG Antibody Conformations in the Gas Phase

“…[18][19][20][21][22][23][24][25] Native MS can be hyphenated with IM;the resulting ion mobility (IM)-MS method offers an extra dimension enabling shape information on the investigated proteins.I M-MS allows for derivation of topological information of proteins through calculating their collisional cross section (CCS). [18][19][20][21][22][23][24][25] Native MS can be hyphenated with IM;the resulting ion mobility (IM)-MS method offers an extra dimension enabling shape information on the investigated proteins.I M-MS allows for derivation of topological information of proteins through calculating their collisional cross section (CCS).…”

Structural Mass Spectrometry of Membrane Proteins within Their Native Lipid Environments

“…Other structural MS techniques need to catch‐up because they can offer different and/or complementary information to XL‐MS, enabling a more developed picture of native membrane protein function and structure to be achieved. This is particularly important as hybrid MS has established ways to combine data from multiple structural MS and molecular modelling techniques to build highly informative models of protein structure and function . Impressive achievements in the use of footprinting techniques, such as HDX‐MS and FPOP‐MS, have been presented in this article which forecasts a promising future for their continued development and success in this field, where the demonstration of nMS and nMS‐IMS of membrane proteins within native nanodiscs is likely forthcoming.…”

A Mass‐Spectrometry‐Based Modelling Workflow for Accurate Prediction of IgG Antibody Conformations in the Gas Phase

“…Native mass spectrometry (MS) has recently emerged as a powerful method for interrogating proteins and their complexes, providing valuable information about their stoichiometry and topology . Native MS can be hyphenated with IM; the resulting ion mobility (IM)‐MS method offers an extra dimension enabling shape information on the investigated proteins.…”

“…Native mass spectrometry (MS) has recently emerged as ap owerful method for interrogating proteins and their complexes,p roviding valuable information about their stoichiometry and topology. [18][19][20][21][22][23][24][25] Native MS can be hyphenated with IM;the resulting ion mobility (IM)-MS method offers an extra dimension enabling shape information on the investigated proteins.I M-MS allows for derivation of topological information of proteins through calculating their collisional cross section (CCS). CCS is described as the rotationally averaged cross section of am olecule and is calculated based on the overall size and molecular architecture.…”