Hybrid liquid biphasic system for cell disruption and simultaneous lipid extraction from microalgae Chlorella sorokiniana CY-1 for biofuel production

Abstract

BackgroundThe extraction of lipids from microalgae requires a pretreatment process to break the cell wall and subsequent extraction processes to obtain the lipids for biofuels production. The multistep operation tends to incur high costs and are energy intensive due to longer process operations. This research work applies the combination of radicals from hydrogen peroxide with an organic solvent as a chemical pretreatment method for disrupting the cell wall of microalgae and simultaneously extracting lipids from the biomass in a one-step biphasic solution.ResultSeveral parameters which can affect the biphasic system were analyzed: contact time, volume of solvent, volume ratio, type of organic solvent, biomass amount and concentration of solvents, to extract the highest amount of lipids from microalgae. The results were optimized and up to 83.5% of lipid recovery yield and 94.6% of enhancement was successfully achieved. The results obtain from GC-FID were similar to the analysis of triglyceride lipid standard.ConclusionThe profound hybrid biphasic system shows great potential to radically disrupt the cell wall of microalgae and instantaneously extract lipids in a single-step approach. The lipids extracted were tested to for its comparability to biodiesel performance.

Field-friendly MUNNG® optima simple test kit for quick qualitative assessment of iodine and iron presence in double-fortified salt

“…Principle: The optima kit follows the basic principle of the release of iron-thiocyanate and iodine-starch interaction under acidic conditions (23). The kit is formulated based on the reaction of ferrous iron and peroxides ii reactions with hydrogen peroxide, in the presence/absence of oxygen (24)(25)(26).…”

Numerical study on the internal fluid mixing and its influencing mechanisms of the wave-driven floating photobioreactor for microalgae production

“…However, applications of the floating PBRs that use waves as the sole energy for culture mixing may be seriously challenged by the intermittent waves, which could produce very poor mixing under poor wave conditions (Yew et al, 2019). Since sufficient mixing could reduce cell settling, facilitate efficient mass transfer and improve the light-dark cycle frequency of algal cells for their efficient cultivation (Gao et al, 2015), the poor mixing from low wave conditions would significantly reduce the biomass productivity of the floating PBRs and even cause the collapse of their cultures (Bitog et al, 2011).…”

Highly selective etherification of fructose and 5‐hydroxymethylfurfural over a novel Pd‐Ru/MXene catalyst for sustainable liquid fuel production

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“…1,2 The catalytic transformation of lignocellulosic biomass into diesel-miscible biofuel is considered to be a sustainable route for energy production and has recently attracted immense attention. [3][4][5][6][7][8] Especially, bio-oil-based oxygencontaining organic compounds are successfully upgraded over different catalysts. 1,[9][10][11] Starch-rich biomass conversion can lead to the production of 5-hydroxymethyl-2-furfuraldehyde (HMF) that can then be catalytically converted into the 5-ethoxymethyl-2-furfural (EMF), levulinic acid (LA), 2,5-furandicarboxylic acid (FDCA), 5-formyl-2-carboxylic acid (FFCA), 2,5-furan dimethanol (FDM), and maleic anhydride (MA) diesel-miscible biofuels.…”