Hybrid hydrogel-aligned carbon nanotube scaffolds to enhance cardiac differentiation of embryoid bodies

Ahadian, Samad; Yamada, Shukuyo; Ramón‐Azcón, Javier; Estili, Mehdi; Liang, Xiaobin; Nakajima, Ken; Shiku, Hitoshi; Khademhosseini, Ali; Matsue, Tomokazu

doi:10.1016/j.actbio.2015.11.047

Cited by 156 publications

(110 citation statements)

References 44 publications

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“…For cardiac tissue engineering, other materials such as reduced graphene oxide or carbon nanotubes were incorporated into GelMA hydrogel to increase its electrical conductivity for maturation of cardiomyocytes. Upon electrical stimulation, cardiomyocytes encapsulated in such hybrid hydrogels demonstrate a higher expression of cardiac markers and faster spontaneous beating than those encapsulated in the pristine GelMA hydrogels [87,88]. GelMA/black phosphorus hybrid hydrogel could also be a promising scaffold for such application [89].…”

Section: Synthesis Properties and Recent Biomedical Applications Of Phmentioning

confidence: 99%

Recent Advances in Photo-Crosslinkable Hydrogels for Biomedical Applications

et al. 2019

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Photo-crosslinkable hydrogels have recently attracted significant scientific interest. Their properties can be manipulated in a spatiotemporal manner through exposure to light to achieve the desirable functionality for various biomedical applications. This review article discusses the recent advances of the most common photo-crosslinkable hydrogels, including poly(ethylene glycol) diacrylate, gelatin methacryloyl and methacrylated hyaluronic acid, for various biomedical applications. We first highlight the advantages of photopolymerization and discuss diverse photosensitive systems used for the synthesis of photo-crosslinkable hydrogels. We then introduce their synthesis methods and review their latest state of development in biomedical applications, including tissue engineering and regenerative medicine, drug delivery, cancer therapies and biosensing. Lastly, the existing challenges and future perspectives of engineering photo-crosslinkable hydrogels for biomedical applications are briefly discussed.

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Section: Synthesis Properties and Recent Biomedical Applications Of Phmentioning

confidence: 99%

Recent Advances in Photo-Crosslinkable Hydrogels for Biomedical Applications

et al. 2019

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“…In particular,m icropatterned electrodes enable the precise positioning of oriented nanofillers in thin hydrogel films to afford hierarchical structures that are generally difficult to access by other methods.Itshould be noted that electric fields possibly cause undesired electrochemical decomposition and electrophoresis.T oa void these problems,t he conditions,s uch as voltage, current frequency, and sample thickness,o ften need to be optimized. Thus far, electric orientation has been used for the synthesis of anisotropic hydrogels containing functionalized carbon nanotubes, [42][43][44] clay nanosheets, [45,46] silver nanowires, [47] silk nanofibers, [48] and barium titanate particles. [49] Similar to electric fields,m agnetic fields can be used to afford anisotropic hydrogels,w here an anofiller is oriented with its easy magnetization axis parallel or antiparallel to the applied magnetic field (Figure 2b).…”

Section: Hydrogels With Oriented Nanofillersmentioning

confidence: 99%

“…[18,[88][89][90][91] Ty pically,a na queous solution of an anionic polymer is filled in ac ontainer, and one end of the container is put in contacted with an aqueous solution containing multivalent cations so that the cationic ions diffuse directionally.A st he diffusion proceeds,t he anionic polymer chains are crosslinked anisotropically to afford ah ydrogel with an oriented polymer-chain network. Va rious anionic polymers have been used as components of such networks, shear forces collagen nanofibers [23] cellulose nanofibers [24] lamellar bilayer [25][26][27] imogolite nanotubes [33] graphene oxide [34] clay nanosheets [32] graphene oxide [34] -s ynthetic peptide nanofibers [19,20] collagen nanofibers [21,23] electric fields silk nanofibers [48] clay nanosheets [46] -f unctionalized carbon nanotubes [42][43][44] silk nanofibers [48] magnetic fields barium ferrite particles [53,55] graphene oxide [70] titanate nanosheets [71] alumina platelets [60] titanate nanosheets [72] magnetite particles [52] micelles [66,67] -Section 2.2 Hydrogels with oriented polymerchain networks compression or stretching alginate/acrylic polymer [81] acrylic polymer [82] poly(vinyl alcohol) [84,85] -s cleroglucan [87] poly(vinyl alcohol) [80] collagen nanofibers…”

Section: Hydrogels With Oriented Polymer-chain Networkmentioning

confidence: 99%

“…[39, 42-44, 47, 70, 75, 123] In particular,much attention has recently been focused on their application as smart cell-culture media, in which cells can be stimulated electrically.M atsue,K hademhosseini, and co-workers reported am ethacrylated gelatin based hydrogel (Figure 6c)containing carbon nanotubes (CNTs) that were anisotropically oriented by applying an electric field (Section 2.1). [42][43][44] Thee lectrical conduction of the hydrogel showed an anisotropy of about 40. As amedium for the growth of skeletal muscle cells,t he hydrogel with vertically oriented CNTs afforded al arger number of functional myofibers than those with randomly oriented and horizontally oriented CNTs.I na ddition, the cell growth was enhanced by applying electrical stimulation along the oriented CNTs.…”

Section: Anisotropic Cell Culturesmentioning

confidence: 99%

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Synthesis of Anisotropic Hydrogels and Their Applications

2018

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Owing to their water-rich structures, which are similar to those of biological tissues, hydrogels have long been regarded as promising scaffolds for artificial tissues and organs. However, in terms of the structural anisotropy, most synthetic hydrogels are substantially different from biological systems. Synthetic hydrogels are usually composed of randomly oriented three-dimensional polymer networks whereas biological systems adopt anisotropic structures with hierarchically integrated building units. Such anisotropic structures often play essential roles in biological systems to exhibit particular functions. In this context, anisotropic hydrogels provide an entry point for exploring biomimetic applications of hydrogels. Reflecting these aspects, an increasing number of studies on anisotropic hydrogels have been reported recently. This Minireview highlights the use and perspectives of these anisotropic hydrogels, particularly focusing on their preparation, structures, and applications.

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“…Recently, nano composite technology has become a particularly hot topic because of its potential to improve materials properties (Gaharwar et al 2013(Gaharwar et al , 2014Ahadian et al 2016;Thakur et al 2016). Carbon nanotubes (CNTs) are typical carbon-based nanomaterials, which have been incorporated into photo-cross-linked gelatin methacryloyl hydrogels.…”

Section: Introductionmentioning

confidence: 99%

Facile modulation of cell adhesion to a poly(ethylene glycol) diacrylate film with incorporation of polystyrene nano-spheres

Wang

et al. 2016

Biomed Microdevices

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Poly(ethylene glycol) diacrylate (PEGDA) is a common hydrogel that has been actively investigated for various tissue engineering applications owing to its biocompatibility and excellent mechanical properties. However, the native PEGDA films are known for their bio-inertness which can hinder cell adhesion, thereby limiting their applications in tissue engineering and biomedicine. Recently, nano composite technology has become a particularly hot topic, and has led to the development of new methods for delivering desired properties to nanomaterials. In this study, we added polystyrene nanospheres (PS) into a PEGDA solution to synthesize a nanocomposite film and evaluated its characteristics. The experimental results showed that addition of the nanospheres to the PEGDA film not only resulted in modification of the mechanical properties and surface morphology but further improved the adhesion of cells on the film. The tensile modulus showed clear dependence on the addition of PS, which enhanced the mechanical properties of the PEGDA-PS film. We attribute the high stiffness of the hybrid hydrogel to the formation of additional cross-links between polymeric chains and the nanosphere surface in the network. The effect of PS on cell adhesion and proliferation was evaluated in L929 mouse fibroblast cells that were seeded on the surface of various PEGDA-PS films.Cells density increased with a larger PS concentration, and the cells displayed a spreading morphology on the hybrid films, which promoted cell proliferation. Impressively, cellular stiffness could also be modulated simply by tuning the concentration of nano-spheres. Our results indicate that the addition of PS can effectively tailor the physical and biological properties of PEGDA as well as the mechanical properties of cells, with benefits for biomedical and biotechnological applications.

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Hybrid hydrogel-aligned carbon nanotube scaffolds to enhance cardiac differentiation of embryoid bodies

Cited by 156 publications

References 44 publications

Recent Advances in Photo-Crosslinkable Hydrogels for Biomedical Applications

Recent Advances in Photo-Crosslinkable Hydrogels for Biomedical Applications

Synthesis of Anisotropic Hydrogels and Their Applications

Facile modulation of cell adhesion to a poly(ethylene glycol) diacrylate film with incorporation of polystyrene nano-spheres

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