Hybrid extracellular vesicles for drug delivery

Ducrot, Coline; Loiseau, Stanislas; Wong, Christophe; Madec, Elise; Volatron, Jeanne; Piffoux, Max

doi:10.1016/j.canlet.2023.216107

Cited by 16 publications

(16 citation statements)

References 37 publications

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“…Thus, milk vesicles (including EVs) were recently applied to form hybrids with cargo-bearing lipid particles for oral delivery (Patent #WO2021142336A1). This method can overcome the disadvantages of lipid particles and EVs when used alone as tNA delivery systems [ 306 ]. The advantage of this approach is that lipid particle loading strategies tend to be standard, unlike EVs [ 306 ].…”

Section: Clinical Trials and Some Recent Patents Related To The Ev Ap...mentioning

confidence: 99%

“…This method can overcome the disadvantages of lipid particles and EVs when used alone as tNA delivery systems [ 306 ]. The advantage of this approach is that lipid particle loading strategies tend to be standard, unlike EVs [ 306 ]. Simultaneously, EVs can provide tissue specificity for the hybrid system.…”

Section: Clinical Trials and Some Recent Patents Related To The Ev Ap...mentioning

confidence: 99%

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Extracellular Vesicles for Therapeutic Nucleic Acid Delivery: Loading Strategies and Challenges

Oshchepkova

Zenkova

Vlassov

2023

IJMS

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Extracellular vesicles (EVs) are membrane vesicles released into the extracellular milieu by cells of various origins. They contain different biological cargoes, protecting them from degradation by environmental factors. There is an opinion that EVs have a number of advantages over synthetic carriers, creating new opportunities for drug delivery. In this review, we discuss the ability of EVs to function as carriers for therapeutic nucleic acids (tNAs), challenges associated with the use of such carriers in vivo, and various strategies for tNA loading into EVs.

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Section: Clinical Trials and Some Recent Patents Related To The Ev Ap...mentioning

confidence: 99%

Section: Clinical Trials and Some Recent Patents Related To The Ev Ap...mentioning

confidence: 99%

Extracellular Vesicles for Therapeutic Nucleic Acid Delivery: Loading Strategies and Challenges

Oshchepkova

Zenkova

Vlassov

2023

IJMS

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“…As promising alternatives to EVs, hybrid membrane nanovesicles (HMNVs) have emerged as advanced platforms for the targeted delivery of different diagnostic agents and therapeutics. [ 82 ] Strikingly, compared to the natural simplex EVs, the integration of natural source cells‐derived functions and artificial modification potentials can endow the HMNVs with precise targeting ability, sufficient production yield, high drug loading capacity, as well as possible additional multiple functions. However, such cutting‐edge hybrid membrane vesicles are still in their beginning stages and have not been applied in clinical practice.…”

Section: Ongoing Challenges and Future Perspectives Of Hmnvsmentioning

confidence: 99%

Beyond Extracellular Vesicles: Hybrid Membrane Nanovesicles as Emerging Advanced Tools for Biomedical Applications

Sun,

Yang,

Fan

et al. 2023

Advanced Science

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Extracellular vesicles (EVs), involved in essential physiological and pathological processes of the organism, have emerged as powerful tools for disease treatment owing to their unique natural biological characteristics and artificially acquired advantages. However, the limited targeting ability, insufficient production yield, and low drug‐loading capability of natural simplex EVs have greatly hindered their development in clinical translation. Therefore, the establishment of multifunctional hybrid membrane nanovesicles (HMNVs) with favorable adaptability and flexibility has become the key to expanding the practical application of EVs. This timely review summarizes the current progress of HMNVs for biomedical applications. Different HMNVs preparation strategies including physical, chemical, and chimera approaches are first discussed. This review then individually describes the diverse types of HMNVs based on homologous or heterologous cell membrane substances, a fusion of cell membrane and liposome, as well as a fusion of cell membrane and bacterial membrane. Subsequently, a specific emphasis is placed on the highlight of biological applications of the HMNVs toward various diseases with representative examples. Finally, ongoing challenges and prospects of the currently developed HMNVs in clinical translational applications are briefly presented. This review will not only stimulate broad interest among researchers from diverse disciplines but also provide valuable insights for the development of promising nanoplatforms in precision medicine.

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“…In this phase, the particle is cloaked with plasma opsonins that act as beacons, marking them for clearance and lysosomal degradation. 108 To circumvent this challenge, pH- Abraxane EP2481405A1 PEGylated albumin-taxoid nanoparticles [104] Polymeric nanoparticles US20190133963A1 PLA-PEG-PPG-PEG multi block copolymer coated polymeric nanoparticles [105] Red blood cells AU2023203791A1 Doxorubicin-loaded liposomes hitchhiked on RBC [18] Apolipoprotein E (ApoE) US8288335B2 Apolipoprotein E (ApoE) coated nanoparticles [106] MEHTA and SHENDE…”

Section: Dysopsonic Protein Coatingmentioning

confidence: 99%

“…Nonetheless, the initial uptake of nanoparticles by cells is initially mediated through processes like phagocytosis or endocytosis. In this phase, the particle is cloaked with plasma opsonins that act as beacons, marking them for clearance and lysosomal degradation 108 . To circumvent this challenge, pH‐responsive liposomes are meticulously engineered to remain steadfast in the neutral, physiologically‐balanced pH levels present in the bloodstream.…”

Section: Ph‐sensitive Lipidsmentioning

confidence: 99%

Evasion of opsonization of macromolecules using novel surface‐modification and biological‐camouflage‐mediated techniques for next‐generation drug delivery

Mehta,

Shende

2023

Cell Biochemistry & Function

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Opsonization plays a pivotal role in hindering controlled drug release from nanoformulations due to macrophage‐mediated nanoparticle destruction. While first and second‐generation delivery systems, such as lipoplexes (50–150 nm) and quantum dots, hold immense potential in revolutionizing disease treatment through spatiotemporal controlled drug delivery, their therapeutic efficacy is restricted by the selective labeling of nanoparticles for uptake by reticuloendothelial system and mononuclear phagocyte system via various molecular forces, such as electrostatic, hydrophobic, and van der Waals bonds. This review article presents novel insights into surface‐modification techniques utilizing macromolecule‐mediated approaches, including PEGylation, di‐block copolymerization, and multi‐block polymerization. These techniques induce stealth properties by generating steric forces to repel micromolecular‐opsonins, such as fibrinogen, thereby mitigating opsonization effects. Moreover, advanced biological methods, like cellular hitchhiking and dysopsonic protein adsorption, are highlighted for their potential to induce biological camouflage by adsorbing onto the nanoparticulate surface, leading to immune escape. These significant findings pave the way for the development of long‐circulating next‐generation nanoplatforms capable of delivering superior therapy to patients. Future integration of artificial intelligence‐based algorithms, integrated with nanoparticle properties such as shape, size, and surface chemistry, can aid in elucidating nanoparticulate‐surface morphology and predicting interactions with the immune system, providing valuable insights into the probable path of opsonization.

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Hybrid extracellular vesicles for drug delivery

Cited by 16 publications

References 37 publications

Extracellular Vesicles for Therapeutic Nucleic Acid Delivery: Loading Strategies and Challenges

Extracellular Vesicles for Therapeutic Nucleic Acid Delivery: Loading Strategies and Challenges

Beyond Extracellular Vesicles: Hybrid Membrane Nanovesicles as Emerging Advanced Tools for Biomedical Applications

Evasion of opsonization of macromolecules using novel surface‐modification and biological‐camouflage‐mediated techniques for next‐generation drug delivery

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