Frequency regulation of wind turbines can improve the stability of the power system. However, it would cause generator torque fluctuation, increasing the risk of fatigue load. Previous research works were restricted to inertial and droop responses. Less attention has been paid to striking a balance between frequency regulation and fatigue load. To overcome these, a hybrid control strategy is proposed to consider both frequency response characteristic and fatigue load mitigation. First, a small signal linearization model is built to reveal the impact of the correlation mechanism of frequency regulation on drive train torque load. Second, a multivariable cost function is constructed to optimize the proportional integral (PI) controller, which combines the total fluctuation with the dispersion of the fatigue load and frequency. Then, a hybrid controller based on PI control optimized by particle swarm optimization algorithm and active disturbance rejection control is designed to restrain rapid frequency changes as well as fatigue torque fluctuation simultaneously. Several experiments are performed to verify the significance of the proposed method under different scenarios. Compared with the existing methods, the proposed hybrid control exhibits superiority in improving frequency response and fatigue load mitigation.