Hybrid-cell membrane-coated nanocomplex-loaded chikusetsusaponin IVa methyl ester for a combinational therapy against breast cancer assisted by Ce6

Abstract

Breast cancer is the most common cancer in women, which occurs mostly with poor outcomes. Our objective was to endow synthetic lethality to phytoconstituent chikusetsusaponin IVa methyl ester (CSME, S),...

Engineered cancer cell membranes: An emerging agent for efficient cancer theranostics

“…developed a drug delivery system based on hybrid CCM‐functionalized liposomes that were loaded with the cytotoxic compound chikusetsusaponin IVa methyl ester (CSME) and photosensitizer chlorin e6 (Ce6) for combinational therapy. [ 49 ] In their approach, the hybrid CCMs derived from RBCs and 4T1 cells were fused into the CSME‐loaded liposomes under sonication to confer the liposomes with specific targeting ability and extended blood circulation time. Subsequently, the Ce6 was loaded into the bilayer and cavity structure of the liposomes, followed by further modification of Arg‐Gly‐Asp (RGD) onto the liposome surface to strengthen their targeting ability.…”

Hybrid Membrane-Coated Biomimetic Nanoparticles (HM@BNPs): A Multifunctional Nanomaterial for Biomedical Applications

“…A recent study showed that, compared with their free forms, RBC membrane modification can mediate a greater enhancement of the cell internalization of drugs . RBC membranes are the most popular natural carriers and can efficiently combine with other membranes and regulate the circulation time of HM@BNPs …”

“…However, the main limitations faced by conventional phototherapeutic agents include bioadhesion and opsonization by serum proteins in the bloodstream, rapid recognition and clearance by immune sentinels and mononuclear phagocyte system (MPS), and low accumulation in action sites . Cell-membrane camouflaging has great potential to overcome these issues due to their abundant surface proteins and biological features, including a lack of immunogenicity, superior blood circulation, or homologous targeting, along with the preservation of the photophysical properties of phototherapeutic nanoparticles . For instance, the long circulation time of RBC membranes is of great significance for improving the performance of photobased applications. ,− The adhesion properties of platelets can improve the inhomogeneous distribution of phototherapeutic agents in phototherapy, as the postinjury feedback caused by thermal damage leads to additional recruitment of platelets and then promotes the targeting ability of delivery system .…”

“…According to the characteristics of the cell membrane, the fusion coating of the erythrocyte membrane and cancer cell membrane can be applied on the surface of nanoparticles loaded with photosensitizers, to efficiently target tumor sites, especially tumor cells that survive deep in solid tumors, and ultimately achieve complete tumor elimination by PDT . Xie et al fabricated a nanocomplex that fused RBCs and 4T1 cells into liposomes, loading the compound chikusetsusaponin IVa methyl ester (CSME/S, a traditional Chinese medicine) and chlorin e6 (Ce6/C, a common photosensitizer in PDT) for the combined chemotherapy/photodynamic therapy of breast cancer . The study of cell uptake showed that the red fluorescence intensity of the LSC group (without membrane coating) in macrophages was notably stronger than that of the LSCMR group, which implied that the hybrid membrane could reduce the uptake of the grid endothelial system.…”

The Current Status of Chlorin e6-Based Nanoscale Delivery Systems for Cancer Therapy

“…What is exciting is that with the continuous advancement of bionic technology, a hybrid cell membrane (HMC) is formed by fusing cell membranes from different sources, which can integrate different membrane functions into a bionic platform. For instance, Xie et al [55] fuses the RBCm and the CCM into a mixed cell membrane to assemble a liposome-based nanoparticle (LSCMR NPs) containing the photosensitizer Ce6 and the chemotherapeutic drug chikusetsusaponin IVa methyl ester. According to SEM and DLS analysis, the zeta potential and average diameter of LSCMR NPs increased slightly.…”