Hybrid breath figure method: A new insight in Petri dishes for cell culture

Huang, Chuixiu; Shen, Xiantao; Liu, Xiaojie; Chen, Zhiliang; Shu, Baolian; Wan, Libin; Liu, Huajing; He, Jun

doi:10.1016/j.jcis.2019.01.074

Cited by 20 publications

(14 citation statements)

References 41 publications

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“…The development of PS as a material for cell culture was described by Lerman et al [41]. The question of how to facilitate cell behavior on commercial tissue culture PS has been addressed by Huang et al [42]. His team invented 3D honeycomb-patterned Petri dishes with incorporated polyethylene glycol (PEG) using a direct breath figure in which HCP is a part of the substrate and no additional polymer solution is needed.…”

Section: Introductionmentioning

confidence: 99%

Cell Behavior of Primary Fibroblasts and Osteoblasts on Plasma-Treated Fluorinated Polymer Coated with Honeycomb Polystyrene

et al. 2021

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The development of new biocompatible polymer substrates is still of interest to many research teams. We aimed to combine a plasma treatment of fluorinated ethylene propylene (FEP) substrate with a technique of improved phase separation. Plasma exposure served for substrate activation and modification of surface properties, such as roughness, chemistry, and wettability. The treated FEP substrate was applied for the growth of a honeycomb-like pattern from polystyrene solution. The properties of the pattern strongly depended on the primary plasma exposure of the FEP substrate. The physico-chemical properties such as changes of the surface chemistry, wettability, and morphology of the prepared pattern were determined. The cell response of primary fibroblasts and osteoblasts was studied on a honeycomb pattern. The prepared honeycomb-like pattern from polystyrene showed an increase in cell viability and a positive effect on cell adhesion and proliferation for both primary fibroblasts and osteoblasts.

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Section: Introductionmentioning

confidence: 99%

Cell Behavior of Primary Fibroblasts and Osteoblasts on Plasma-Treated Fluorinated Polymer Coated with Honeycomb Polystyrene

et al. 2021

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“…According to the dimensions of the interface between cell and substrate materials, the mainstream in vitro cell culture techniques are mainly divided into 2D culture and 3D culture [5,6,7]. In 2D culture, the surface-modified substrate usually provides an opening space for cells to attach on, and cells are immersed with the culture medium directly [8]. Different substrate materials could induce distinguishing growth performances of cells [6,8].…”

Section: Introductionmentioning

confidence: 99%

“…In 2D culture, the surface-modified substrate usually provides an opening space for cells to attach on, and cells are immersed with the culture medium directly [8]. Different substrate materials could induce distinguishing growth performances of cells [6,8]. 2D cell culture is a necessary method for in vitro cell culture, yet it still has some limitations for mimicking an in vivo microenvironment [9].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions

2019

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The interactions between substrate materials and cells usually play an important role in the hydrogel-based 3D cell cultures. However, the hydrogels that are usually used could not be parametrically regulated, especially for quantitatively regulating the spatial distribution of the adhesion sites for cells in 3D. Here, we employed the semisynthetic hydrogel consisting of maleimide-dextran, Arg-Gly-Asp (RGD) peptides, and cell degradable crosslinkers to biochemically characterize the evolutionary behaviors of NIH–3T3 fibroblasts and C2C12 cells in 3D. Moreover, by comparing the cell-adhesive efficacy of 3D dextran hydrogels with four different RGD clustering rates, we explored the underlying regulation law of C2C12 connections and 3T3 aggregations. The results showed that mal-dextran hydrogel could promise cells stable viability and continuous proliferation, and induce more self-organized multicellular structures relative to 2D culture. More importantly, we found that RGD-clustered mal-dextran hydrogel has the advantage of enhancing C2C12 cell elongation and the breadthwise-aggregated connection, and promoting the 3T3 cell aggregating degree compared to that with homogenous RGD. Further, the advantages of RGD clustering hydrogel could be amplified by appropriately reducing RGD concentration. Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.

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“…In recent years, the application of BF method in the preparation of organic/inorganic hybrid materials has been developed. [ 88‐90 ] Graphene/Au@ZnO photoelectrode has been prepared via BF method by Sun et al . [ 91 ] Yuan et al .…”

Section: Functional Modification Of Bf Membranesmentioning

confidence: 99%

Ordered Honeycomb‐Pattern Membrane^†

Yuan

Dai

et al. 2020

Chin. J. Chem.

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Ordered porous membranes have wide and important application prospects in the fields of filtration, separation, catalysis, biomedicine, etc., and have attracted growing interest in the field of material science. Among diverse membrane preparation approaches, breath pattern method has been widely concerned due to its simplicity in the preparation, ease in tuning the structure/property and functionality of formed membranes, and broad utility for various polymers. With the development of material design, the application spectrum of breath figure membrane has been greatly expanded. However, since the pore morphology of porous membrane is influenced by many factors, the controlling mechanism of normalization has not been clear yet. In this paper, the research progress of film-forming materials in recent years is reviewed, and the correlation between pore formation and pore morphology is discussed in details. This information will provide a systematic and in-depth perspective for research in this filed and an important guideline for the preparation of the ordered porous films with divergent applications. Hua Yuan (left) is an associate professor in College of Materials Science and Engineering, Qingdao University, China. She received her doctorate from Shandong University in China in 2013. She is mainly engaged in the research of carbon fiber, composites and microfiltration membrane. Guangzhen Li (middle) is a graduate student in the College of Materials Science and Engineering, Qingdao University, China. He graduated with a bachelor's degree in engineering from Qingdao University in 2019. His current research focuses on the structural design and functionalization of membrane materials. Enhao Dai (right) is a graduate student of the College of Materials Science and Engineering of Qingdao University. He received a bachelor's degree in engineering from Yantai University in 2018. Currently, he mainly studies carbon fiber modification.

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Hybrid breath figure method: A new insight in Petri dishes for cell culture

Cited by 20 publications

References 41 publications

Cell Behavior of Primary Fibroblasts and Osteoblasts on Plasma-Treated Fluorinated Polymer Coated with Honeycomb Polystyrene

Cell Behavior of Primary Fibroblasts and Osteoblasts on Plasma-Treated Fluorinated Polymer Coated with Honeycomb Polystyrene

Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions

Ordered Honeycomb‐Pattern Membrane^†

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Hybrid breath figure method: A new insight in Petri dishes for cell culture

Cited by 20 publications

References 41 publications

Cell Behavior of Primary Fibroblasts and Osteoblasts on Plasma-Treated Fluorinated Polymer Coated with Honeycomb Polystyrene

Cell Behavior of Primary Fibroblasts and Osteoblasts on Plasma-Treated Fluorinated Polymer Coated with Honeycomb Polystyrene

Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions

Ordered Honeycomb‐Pattern Membrane†

Contact Info

Product

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About

Ordered Honeycomb‐Pattern Membrane^†