2020
DOI: 10.3389/fbioe.2020.00484
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Hybrid Bioprinting of Chondrogenically Induced Human Mesenchymal Stem Cell Spheroids

Abstract: To date, the treatment of articular cartilage lesions remains challenging. A promising strategy for the development of new regenerative therapies is hybrid bioprinting, combining the principles of developmental biology, biomaterial science, and 3D bioprinting. In this approach, scaffold-free cartilage microtissues with small diameters are used as building blocks, combined with a photo-crosslinkable hydrogel and subsequently bioprinted. Spheroids of human bone marrow-derived mesenchymal stem cells (hBM-MSC) are… Show more

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Cited by 74 publications
(79 citation statements)
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“…Taking again the example of spheroids/microtissues, bioprinting of these building blocks exhibits distinct differences from single cell bioprinting and poses challenges in terms in terms of homogeneity of spheroid distribution in the resulting macro-tissue. Differentiated cartilaginous microtissues have recently been bioprinted in GelMA bioinks [ 151 ]. Despite some indications of positive viability readouts [ 152 ] as compared to single cell suspensions [ 152 ], there is a lack of dedicated studies on bioprinting process parameters and their impact on viability of the printed spheroids.…”
Section: Delivering De Implants – the Path To The Patientmentioning
confidence: 99%
“…Taking again the example of spheroids/microtissues, bioprinting of these building blocks exhibits distinct differences from single cell bioprinting and poses challenges in terms in terms of homogeneity of spheroid distribution in the resulting macro-tissue. Differentiated cartilaginous microtissues have recently been bioprinted in GelMA bioinks [ 151 ]. Despite some indications of positive viability readouts [ 152 ] as compared to single cell suspensions [ 152 ], there is a lack of dedicated studies on bioprinting process parameters and their impact on viability of the printed spheroids.…”
Section: Delivering De Implants – the Path To The Patientmentioning
confidence: 99%
“…Very few studies used low cell densities, but they achieved good chondrogenic results [ 154 , 158 ]. For BM-MSCs, the range of densities is comparable to that of chondrocytes, starting from 4 × 10 6 cells/mL [ 175 ] and increasing to as high as 20 × 10 6 cells/mL [ 163 ]; most of the studies were between those two values, thus achieving the best chondrogenic induction possible [ 106 , 162 , 165 , 166 ]. Recently, some research aimed to compare two very low cell densities, 1 and 2 × 10 6 cells/mL, to assess better options for obtaining good chondrogenesis, and they showed that the lowest density seemed to be optimal [ 60 ].…”
Section: Bioextrusion Processes For Cartilage Tissue Engineeringmentioning
confidence: 99%
“…In contrast, other biomaterials, such as gelatin, silk, or collagen, can improve cell nesting and survival [ 154 , 155 ]. Other studies used cell aggregates or spheroids to seed into the bioink instead of single cells [ 103 , 175 ]. A comparison of embedded spheroids to isolated cells in bioextruded substitutes demonstrated a better survival of cells inside spheroids [ 175 ].…”
Section: Bioextrusion Processes For Cartilage Tissue Engineeringmentioning
confidence: 99%
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