Hybrid Aptamer-Antibody Linked Fluorescence Resonance Energy Transfer Based Detection of Trinitrotoluene

Sabherwal, Priyanka; Shorie, Munish; Pathania, Preeti; Chaudhary, Shilpa; Bhasin, K. K.; Bhalla, Vijayender; Suri, C. Raman

doi:10.1021/ac501388a

Cited by 44 publications

(20 citation statements)

References 26 publications

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“…Very recently, Suri and co-workers reported on using TNT specific aptamer and antibodies tagged with respective FRET pair for explosives detection. 256 The as-developed sensor showed excellent sensitivity with LOD of 0.4 nM. Secondly, researchers are harnessing fluorescent properties of biomaterials such as fluorescent proteins to serve as explosives sensors.…”

Section: Bio-inspired Fluorescent Materials For Explosives Detectionmentioning

confidence: 99%

Fluorescence based explosive detection: from mechanisms to sensory materials

2015

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The detection of explosives is one of the current pressing concerns in global security. In the past few decades, a large number of emissive sensing materials have been developed for the detection of explosives in vapor, solution, and solid states through fluorescence methods. In recent years, great efforts have been devoted to develop new fluorescent materials with various sensing mechanisms for detecting explosives in order to achieve super-sensitivity, ultra-selectivity, as well as fast response time. This review article starts with a brief introduction on various sensing mechanisms for fluorescence based explosive detection, and then summarizes in an exhaustive and systematic way the state-of-the-art of fluorescent materials for explosive detection with a focus on the research in the recent 5 years. A wide range of fluorescent materials, such as conjugated polymers, small fluorophores, supramolecular systems, bio-inspired materials and aggregation induced emission-active materials, and their sensing performance and sensing mechanism are the centerpiece of this review. Finally, conclusions and future outlook are presented and discussed.

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Section: Bio-inspired Fluorescent Materials For Explosives Detectionmentioning

confidence: 99%

Fluorescence based explosive detection: from mechanisms to sensory materials

2015

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“…Chem 3D is a powerful modeling program which can be used to generate 3D models and calculate the structure and properties of molecules. [29]. Considering the structural similarity of EDTA and AAPTS‐COOH, 3D models of EDTA, and AAPTS‐COOH coordinating with Cu 2+ were constructed using Chem 3D software (ChemBio3D Ultra 14.0), respectively.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of multidentate functional monomer for ion imprinting

Chen

Liang

Xing

2020

J of Separation Science

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In this work, N,N,propyl-trimethoxysilane was prepared as a multidentate functional monomer. The 3D model of the monomer coordinating with Cu 2+ indicated that the monomer is able to provide five ligating atoms like ethylenediaminetetraacetic acid-Cu 2+ to complex with Cu 2+ .When Cu 2+ was used as a template ion, the synthesis conditions of Cu 2+ -imprinted polymers were optimized upon orthogonal design. It is interesting to find that Cu 2+imprinted polymer offers a selectivity coefficient of 192.2 when the molar ratio of Cu 2+ to monomer was exactly 1:1. That means there is no excess ligating atom in the ion-imprinted polymer and therefore, the nonspecific adsorption could be avoided.Benefiting from the excellent selectivity of Cu 2+ -imprinted polymer, even if the concentration of Zn 2+ was 25 times that of Cu 2+ , Cu 2+ -imprinted polymer still affords a high selectivity coefficient. Finally, the optimal synthesis conditions for Cu 2+imprinted polymer, except the pH, were adopted to prepare Ni 2+ -imprinted polymer, and Ni 2+ -imprinted polymer also offered satisfying selectivity to Ni 2+ . That implies this multidentate monomer is adaptable in ion imprinting and, the optimal synthesis conditions of Cu 2+ -imprinted polymer except pH are likely suitable for the imprinting of other ions besides Cu 2+ . K E Y W O R D Sion-imprinted polymer, multidentate functional monomer, sol-gel 1356

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“…A significant advantage of the use of aptamers compared to antibody probes is that this approach can be readily adapted to any aptamer/antibody or aptamer/aptamer sandwich assay combination with the number of examples of both continuing to rise in the literature. [45][46][47][48] Furthermore, by exploring different nanoparticle shapes we highlight the versatility of this approach and we also expect the use of different modified DNA sequences binding to the aptamer will expand this approach to other surface-based signal transduction mechanisms such as electrochemistry. 49 As well as developing a new amplification mechanism, it was 15 demonstrated that even further improvements in sensitivity could be achieved compared to previous efforts focusing on a single nanoparticle amplification step.…”

Section: Discussionmentioning

confidence: 99%

Dual Nanoparticle Amplified Surface Plasmon Resonance Detection of Thrombin at Subattomolar Concentrations

2014

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This version is available at https://strathprints.strath.ac.uk/50087/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge.Any correspondence concerning this service should be sent to the AbstractA novel dual nanoparticle amplification approach is introduced for the enhanced surface plasmon resonance (SPR) detection of a target protein at subattomolar concentrations.Thrombin was used as a model target protein as part of a sandwich assay involving an antithrombin (anti-Th) modified SPR chip surface and a thrombin specific DNA aptamer (Thaptamer) whose sequence also includes a polyadenine (A30) tail. Dual nanoparticle (NP) enhancement was achieved with the controlled hybridization adsorption of first polythymine-NP conjugates (T20-NPs) followed by polyadenine-NP's (A30-NPs). Two different nanoparticle shapes (nanorod and quasi-spherical) were explored resulting in four different NP pair combinations being directly compared. It was found that both the order and NP shape were important in optimizing the assay performance. The use of real-time SPR measurements to detect target concentrations as low as 0.1 aM is a ten-fold improvement compared to single NP-enhanced SPR detection methods.

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Hybrid Aptamer-Antibody Linked Fluorescence Resonance Energy Transfer Based Detection of Trinitrotoluene

Cited by 44 publications

References 26 publications

Fluorescence based explosive detection: from mechanisms to sensory materials

Fluorescence based explosive detection: from mechanisms to sensory materials

Synthesis of multidentate functional monomer for ion imprinting

Dual Nanoparticle Amplified Surface Plasmon Resonance Detection of Thrombin at Subattomolar Concentrations

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