Hybrid affinity chromatography of α-galactosidase from Verbascum thapsus L.

Bom, I.J.; Wassenaar, Dick van; Boot, J. H.

doi:10.1016/s0021-9673(98)00104-6

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…They obtained 300 mg thapsuine A and 460 mg thapsuine B. Bom et al (1998) purified α-galactosidase from the roots of V. thapsus L. using hybrid affinity chromatography with good recovery.…”

Section: Active Ingredients Of V Thapsus Lmentioning

confidence: 99%

Common mullein (Verbascum thapsus L.): recent advances in research

Türker

Gürel

2005

Phytother. Res.

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Common mullein (Verbascum thapsus L.) is a medicinal plant readily found in roadsides, meadows and pasture lands and has been used to treat pulmonary problems, inflammatory diseases, asthma, spasmodic coughs, diarrhoea and migraine headaches. Although it has been used medicinally since ancient times, the popularity of common mullein has been increasing commercially for the past few years. Today, the dried leaves and flowers, swallow capsules, alcohol extracts and the flower oil of this plant can easily be found in health stores in the United States. The use of common mullein extracts in folk medicine begun recently to be supported by an increasing number of research studies. This paper thoroughly reviews all the scientific research related to Verbascum thapsus L. including plant tissue cultures and the biological properties of this plant.

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Section: Active Ingredients Of V Thapsus Lmentioning

confidence: 99%

Common mullein (Verbascum thapsus L.): recent advances in research

Türker

Gürel

2005

Phytother. Res.

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“…In 1980, Pascual Teresa, Diaz, andGrande (1978) identified four saponins from commercially available V. thapsus capsules, namely thapsuine A (300 mg), thapsuine B (460 mg), hydroxythapsuine A and hydroxythapsuine B. Bom, van Wassenaar, and Boot (1998) isolated α-galactosidase from the V. thapsus roots with significant yield.…”

Section: Phytochemical Profiling Of V Thapsusmentioning

confidence: 99%

“…Phenylethanoid and lignan glycosides (Warashina et al, 1992), sterones, iridoid glycosides and sesquiterpene acid (Khuroo et al, 1988; Warashina, Miyase, & Ueno, 1991), and verbascoside, a new luteolin glycoside, (Mehrotra et al, 1989) were extracted from whole plants of V. thapsus . In 1980, Pascual Teresa, Diaz, and Grande (1978) identified four saponins from commercially available V. thapsus capsules, namely thapsuine A (300 mg), thapsuine B (460 mg), hydroxythapsuine A and hydroxythapsuine B. Bom, van Wassenaar, and Boot (1998) isolated α‐galactosidase from the V. thapsus roots with significant yield.…”

Section: Introductionmentioning

confidence: 99%

Health‐promoting and disease‐mitigating potential of Verbascum thapsus L. (common mullein): A review

Gupta

Atkinson

Pandey

et al. 2022

Phytotherapy Research

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Medicinal plants represent rich sources of traditional medicines and numerous currently used medicines are either directly or indirectly derived from plants. Verbascum thapsus L. (great mullein or common mullein), a medicinal herb indigenous to northern Africa, western and central Asia, and Europe, has been brought to the Americas and Australia. It has been used as a medicine for lung, skin and throat disorders and has a long history of therapeutic importance, particularly as an astringent and calming agent. Presently, the dried leaves, flowers, various plant extracts and flower oil are used in several formulations within Indian traditional medicine. An extract taken from the roots is useful in minimizing toothache, and it also relieves stiffness and seizures.V. thapsus contains a wide variety of phytoconstituents, such as flavonoids, iridoid, phenylethanoid and phenylpropanoid glycosides, saponins, as well as vitamin C and minerals. The most valuable constituents are coumarin and hesperidin, which possess healing properties. Emerging literature based on experimental studies on V. thapsus demonstrates various biological and pharmacological properties, including antiviral, antioxidant, analgesic, sedative, anti-inflammatory, hypnotic, antibacterial, antifungal, as well as anticancer activities. The present review provides an updated, comprehensive, and critical evaluation of various health-promoting and disease-mitigating properties of V. thapsus.

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“…Ainda segundo os autores, ela se encontra em sementes secas e mantêm a atividade naquelas sementes cuja germinação foi bloqueada. A alfa-galactosidase está presente em sementes germinadas de Tachigalia multijuga (Fialho et al, 2002 a), em sementes de Platymiscium pubescens , em bactéria Cladosporium cladosporoides onde promove a liberação de galactose dos oligossacarídeos da família rafinose (Cruz et al, 1981), no caule de Colocasia esculenta (Chen & Lin-Chu, 1991) e em raiz de Verbascum thapsus (Bom et al, 1998).…”

Section: Introductionunclassified

Caracterização de alfa-galactosidase em embrião e cotilédones de sementes de Platymiscium pubescens Micheli, var. pubecens (tamboril-da-mata)

Lima

Rezende

Borges

et al. 2004

Rev. bras. sementes

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RESUMO -Sementes de Platymiscium pubescens foram colocadas para embeber em água, sendo retiradas amostras para as caracterizações bioquímica e cinética da enzima alfa-galactosidase do eixo embrionário e dos cotilédones. A atividade específica no eixo embrionário aumenta de zero até o tempo de 96 horas de embebição, estabilizando em seguida. A atividade da enzima dos cotilédones mostrou pequeno incremento durante esse mesmo tempo. A alfa-galactosidase do eixo embrionário apresentou atividade máxima no intervalo de pH de 4,5 a 6,0. Por outro lado, para a enzima proveniente dos cotilédones, a maior atividade foi detectada na faixa de 4,0 a 6,0. A temperatura de 55ºC foi a que mais estimulou as atividades da alfa-galactosidase do eixo embrionário e dos cotilédones. As enzimas do eixo embrionário e dos cotilédones mostraram-se termotolerantes, não se alcançando a meia vida na temperatura de 40ºC, no período de 1.500 minutos. A atividade da alfa-galactosidase do eixo embrionário foi inibida por melibiose, CuSO 4 e SDS, enquanto a dos cotilédones foi por todos os efetores, exceto com SDS, CuSO 4 e galactose que tiveram efeito neutro sobre a atividade da alfa-galactosidase dos cotilédones. Os valores de K M para as alfa-galactosidases do embrião e para o cotilédone foram 3,37 e 0,26 mM, respectivamente.Termos para indexação: espécie florestal, alfa-galactosidase, enzima. CARACTERIZATION OF ALFA-GALACTOSIDASE IN EMBRYONIC AXIS AND COTYLEDONS OF Platymiscium pubescens SEEDSABSTRACT: Platymiscium pubescens seeds were placed to soak in water and sampled for biochemistry and kinetic characterizations of embryonic axis and the alfa-galactosidase cotyledon enzyme. The specific activity in the embryonic axis increased from zero to 96 hours of imbibition, stabilizing soon after. The activity of the cotyledon enzyme showed a small increase in the same period. The alfa-galactosidase of the embryonic axis presented its maximum activity in the interval of pH 4.5 to 6.0. On the other hand, for the cotyledon enzyme, the highest activity was detected in the interval of 4.0 to 6.0. The temperature of 55ºC was best to stimulate embryonic axis and cotyledon alfa-galactosidase acticvity. Enzymes of the embryonic axis and the cotyledons were heat tolerant, not reaching the half life at 40ºC, in 1.500 minutes. The embryonic axis alfa-galactosidase activity was inhibited by melibiose, CuSO 4 and SDS, while that of cotyledons was for all the effectors, except for SDS, CuSO 4 and galactose which had neutral effect. The values of K M for the embryo and for the cotyledon alfa-galactosidases were 3.37 and 0.26 mM, respectively.

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Hybrid affinity chromatography of α-galactosidase from Verbascum thapsus L.

Cited by 7 publications

References 12 publications

Common mullein (Verbascum thapsus L.): recent advances in research

Common mullein (Verbascum thapsus L.): recent advances in research

Health‐promoting and disease‐mitigating potential of Verbascum thapsus L. (common mullein): A review

Caracterização de alfa-galactosidase em embrião e cotilédones de sementes de Platymiscium pubescens Micheli, var. pubecens (tamboril-da-mata)

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