Hyaluronic acid (Sperm Select ) improves retention of sperm motility and velocity in normospermic and Oligospermic specimens

Huszár, Gábor; Willetts, Melanie; Corrales, Marcelia

doi:10.1016/s0015-0282(16)54016-3

Cited by 66 publications

(2 citation statements)

References 21 publications

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“…The expansion of the COC may facilitate the extrusion of the oocyte through the ruptured follicle wall during ovulation and assist its capture by the oviductal fimbria and entry into the oviduct (6,7). Moreover, HA and DS proteoglycans may contribute to the success of oocyte fertilization by stabilizing the structure of the egg zona pellucida (8) and by stimulating sperm activation (9) and motility (10,11). Presently, it is not known if COC expansion in vivo is induced directly by gonadotropins or is mediated indirectly by the mural granulosa cells (12).…”

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confidence: 99%

Hyaluronan Synthesis by Mouse Cumulus Cells Is Regulated by Interactions between Follicle-stimulating Hormone (or Epidermal Growth Factor) and a Soluble Oocyte Factor (or Transforming Growth Factor β1)

Tirone

D’Alessandris

Hascall

et al. 1997

Journal of Biological Chemistry

120

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Expansion of the cumulus cell-oocyte complex (COC) in the preovulatory mammalian follicle requires a transient induction of hyaluronan (HA) synthesis by the cumulus cells. We studied the interactions of known factors that regulate this process by isolating compact COCs from mice and inducing their expansion in vitro. Maximum HA synthesis requires either follicle-stimulating hormone (FSH) or epidermal growth factor (EGF) in combination with either a soluble factor(s) produced by the oocyte or transforming growth factor ␤ 1 . FSH (or EGF) exerts its effects during the first 2 h of incubation, before HA synthesis actually begins. The oocyte factor(s) (or transforming growth factor ␤ 1 ) exerts its effects from 2 h onwards and must be continuously present throughout the subsequent ϳ10 h to achieve a maximum level of HA synthesis. FSH stimulates intracellular cAMP synthesis, which correlates with net HA production up to ϳ14 fmol/COC at 5 ng/ml FSH; however, higher concentrations of FSH increase cAMP levels ϳ10-fold higher with no additional effect on HA synthesis. EGF at saturating concentrations for HA synthesis does not stimulate cAMP above basal levels. Tyrosine kinase inhibitors genistein and tyrphostin AG18 nearly abolish the HA synthesis response to EGF and inhibit the response to FSH by ϳ60%, suggesting that a tyrosine kinase activity is involved for both factors, whereas FSH also operates partially through another signaling pathway. Actinomycin D abolishes HA synthesis if added at the beginning of culture and reduces HA synthesis by ϳ50% if added between 6 -12 h when HA synthesis is normally maximal. The results suggest that regulation of HA synthesis is primarily controlled at the transcriptional level.In the mammalian preovulatory follicle, the cumulus oophorus expands dramatically when the cumulus cells (CCs), 1 consisting of a few layers of granulosa cells closely surrounding the oocyte, are induced to synthesize large amounts of hyaluronan (HA) (1). The secreted HA interacts with specific matrix components, thereby forming a highly hydrated and viscoelastic matrix in the intercellular spaces, which results in an ϳ20-fold increase in volume of the cumulus cell-oocyte complex (COC) (2-4). Recent findings show that CCs synthesize a dermatan sulfate (DS) proteoglycan with high molecular weight that also accumulates in the extracellular matrix, most likely through specific binding to HA (5). The expansion of the COC may facilitate the extrusion of the oocyte through the ruptured follicle wall during ovulation and assist its capture by the oviductal fimbria and entry into the oviduct (6, 7). Moreover, HA and DS proteoglycans may contribute to the success of oocyte fertilization by stabilizing the structure of the egg zona pellucida (8) and by stimulating sperm activation (9) and motility (10, 11). Presently, it is not known if COC expansion in vivo is induced directly by gonadotropins or is mediated indirectly by the mural granulosa cells (12). However, the time course of mouse COC expansion and HA synthesis i...

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mentioning

confidence: 99%

Hyaluronan Synthesis by Mouse Cumulus Cells Is Regulated by Interactions between Follicle-stimulating Hormone (or Epidermal Growth Factor) and a Soluble Oocyte Factor (or Transforming Growth Factor β1)

Tirone

D’Alessandris

Hascall

et al. 1997

Journal of Biological Chemistry

120

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“…Studies of human spermatozoa showed that the HBA assay can be an objective, standardized test to evaluate sperm functionality (Szucs et al 2015). Spermatozoa binding to hyaluronan carry fewer chromosomal abnormalities (Yagci et al 2010), are more mature on a developmental level (Huszar et al 1990;Huszar et al 2006;Huszar et al 2007;Lazarevic et al 2010) and demonstrate fewer morphological abnomalities (Szucs et al 2015;Ye et al 2006;Worrilow et al 2004) than non HBA-binding spermatozoa. Human sperm selection through HBA resulted in improved in-vitro fertilization rate (Breznik et al 2013;Erberelli et al 2017, Worrilow et al 2013).…”

Section: Sperm-hyaluronan Binding Assaymentioning

confidence: 99%

Use of a Sperm-Hyaluronan Binding Assay for Evaluation of Sperm Quality in Dromedary Camels

Kotila-Ioannou,

Morrell,

Ntallaris

et al. 2024

Preprint

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Sperm Penetration into a Hyaluronic Acid Polymer as a Means of Monitoring Functional Competence

AITKEN,

BOWIE,

BUCKINGHAM

et al. 1992

Journal of Andrology

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The diagnostic significance of sperm penetration assays based on a commercially available hyaluronate preparation (Sperm Select) has been investigated in the male partners of 77 couples characterized by a normal female partner. Sperm penetration into hyaluronate was highly correlated with the ability of the same sperm populations to penetrate bovine cervical mucus and, moreover, depended on the same attributes of semen quality, including the morphology of the spermatozoa, their number, and their motility as reflected by their mean path velocity. Stepwise multiple regression analyses employing these independent variables generated r values of 0.821 to 0.931, depending on the criterion of hyaluronate penetration used; path velocity was consistently the most informative variable according to the standardized regression coefficients. The relationship between hyaluronate penetration and sperm movement was so close that multiple regression equations could be generated that were capable of accounting for up to 76% of the variance in sperm velocity measurements obtained with a computerized image analysis system. Regression equations could also be generated using the hyaluronate penetration data that could account for 65% of the variance observed in an A23187‐enhanced zona‐free hamster oocyte penetration test, including the successful identification of the subpopulation of patients in whom 0% oocyte penetration had been recorded. Within the same data set, independent variables based on bovine cervical mucus penetration could only account for 43.5% of the variance in sperm‐oocyte fusion. Hyaluronate penetration therefore appears to offer a simple, objective means of generating information on the functional competence of human spermatozoa that should find a role in routine diagnostic services where the more specialized tests are not available.

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Hyaluronic acid (Sperm Select ) improves retention of sperm motility and velocity in normospermic and Oligospermic specimens

Cited by 66 publications

References 21 publications

Hyaluronan Synthesis by Mouse Cumulus Cells Is Regulated by Interactions between Follicle-stimulating Hormone (or Epidermal Growth Factor) and a Soluble Oocyte Factor (or Transforming Growth Factor β1)

Hyaluronan Synthesis by Mouse Cumulus Cells Is Regulated by Interactions between Follicle-stimulating Hormone (or Epidermal Growth Factor) and a Soluble Oocyte Factor (or Transforming Growth Factor β1)

Use of a Sperm-Hyaluronan Binding Assay for Evaluation of Sperm Quality in Dromedary Camels

Sperm Penetration into a Hyaluronic Acid Polymer as a Means of Monitoring Functional Competence

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