Hyaluronic Acid–Povidone-Iodine Compound Facilitates Diabetic Wound Healing in a Streptozotocin-Induced Diabetes Rodent Model

Chen, Rong‐Fu; Wang, Chunting; Chen, Yuhua; Chien, Ching-Ming; Lin, Sin-Daw; Lai, Chung-Sheng; Wang, Ching‐Jen; Kuo, Yur‐Ren

doi:10.1097/prs.0000000000005504

Cited by 29 publications

(31 citation statements)

References 24 publications

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“…Diabetes mellitus (DM) is a prevalent metabolic disease caused by impaired blood glucose control. DM affects approximately 350 million people worldwide, and the incidence of DM is still steadily increasing [1]. Diabetic wounds are one of the complications of DM; delayed wound healing in the lower extremities is prevalent in 15%-25% of DM patients [2,3].…”

Section: Introductionmentioning

confidence: 99%

Supercritical Carbon Dioxide-decellularized Porcine Acellular Dermal Matrix combined with Autologous Adipose-derived Stem Cells: Its Role in Accelerated Diabetic Wound Healing

Chou¹,

Lin²,

Wu³

et al. 2020

Int. J. Med. Sci.

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Diabetes mellitus (DM) causes impaired wound healing by affecting one or more of the biological mechanisms of hemostasis, inflammation, proliferation, and remodeling and a large number of cell types, extracellular components, growth factors, and cytokines. Interventions targeted toward these mechanisms might accelerate the wound healing process. To evaluate the wound healing efficacy of supercritical carbon dioxide (scCO2)-decellularized porcine acellular dermal matrix (ADM) combined with autologous adipose-derived stem cells (ASCs) in streptozotocin (STZ)-induced DM rats. DM was induced by injecting rats with STZ; dorsal full-thickness skin (5 × 5 cm 2) was created and treated with and without ASCs-scCO2-treated ADM to evaluate the wound healing rate through histological examination, fluorescence microscopic observation, and immunohistochemical analysis. In the present study, complete decellularization of the porcine dermal matrix was achieved through scCO2. Isolation of ASCs was conducted and evaluated using CD29 + /CD31 − /CD45 − /CD90 + markers in flow cytometry, which indicated that more than 90% of cells were ASCs. The percentage of cells labeled with CD29 + and CD90 + was found to be 97.50% and 99.69%, respectively. The wound healing rate increased in all groups relative to the group with the DM wound without treatment. DM wound treated with ADM-ASCs showed significantly higher (p < 0.01) wound healing rate than DM wound without treatment. ADM-ASC-treated rats showed significantly increased epidermal growth factor, Ki67, and prolyl 4-hydroxylase and significantly decreased CD45 compared with the group with the DM wound without treatment. The intervention comprising ADM decellularized from porcine skin by using scCO2 and ASCs was proven to improve diabetic wound healing. ADM-ASCs had a positive effect on epidermal regeneration, anti-inflammation, collagen production and processing, and cell proliferation; thus, it accelerated wound healing.

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Section: Introductionmentioning

confidence: 99%

Supercritical Carbon Dioxide-decellularized Porcine Acellular Dermal Matrix combined with Autologous Adipose-derived Stem Cells: Its Role in Accelerated Diabetic Wound Healing

Chou¹,

Lin²,

Wu³

et al. 2020

Int. J. Med. Sci.

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“…The wounding operation was performed four weeks after the STZ injection of four-month-old male Wistar rats. Following former methods, rats received an intraperitoneal injection of freshly prepared STZ (Sigma-Aldrich, St Louis, MO, USA) solution at a dose of 50 mg/kg body weight [30,33]. A dorsal skin wounding defect with an area of 6 × 5 cm 2 involving entire skin excision below the level of the dorsal fascia was used as the wounding defect model [14].…”

Section: Experimental Section 21 Animal Modelmentioning

confidence: 99%

“…Semiquantitative IHC staining was performed by using a horseradish peroxidasediaminobenzidine (HRP-DAB) staining kit (R&D Inc., Minneapolis, MN, USA) following our previous publications [14,30]. Biopsy tissues were collected from the wound margin on days 3 and 10 following the final treatment of the ESWT group and at corresponding time points in the BIO and control groups.…”

Section: Immunohistochemical (Ihc) Staining and Histomorphometric Examentioning

confidence: 99%

“…Therefore, in the present study, we extended our previous studies and investigated whether the promoting effect of ESWT on wound healing is involved in Wnt/β-catenin related pathway by using dorsal wounding in streptozotocin (STZ)-induced diabetic rats [14,30]. 6-bromoindirubin-3'-oxime (BIO), a selective inhibitor of GSK-3 activity that triggers Wnt/β-catenin signaling, was applied to evaluate the wound healing process [31].…”

Section: Introductionmentioning

confidence: 99%

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The Acceleration of Diabetic Wound Healing by Low-Intensity Extracorporeal Shockwave Involves in the GSK-3β Pathway

et al. 2020

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Previous studies have demonstrated that extracorporeal shock wave therapy (ESWT) could accelerate diabetic wound healing and that the inhibition of glycogen synthase kinase-3β (GSK-3β) is involved in epithelial differentiation during wound healing. This study investigated whether the enhancement of diabetic wound healing by ESWT is associated with the GSK-3β-mediated Wnt/β-catenin signaling pathway. A dorsal skin wounding defect model using streptozotocin-induced diabetic rodents was established. Rats were divided into 4 groups: group 1, normal controls without diabetes; group 2, diabetic controls without treatment; group 3, diabetic rats receiving ESWT; and group 4, rats receiving 6-bromoindirubin-3′oxime (BIO), a GSK-3β inhibitor, to trigger Wnt/β-catenin signaling. Tissue samples were collected and analyzed by immunohistochemical (IHC) staining and quantitative RT-PCR. The ESWT and BIO-treated groups both exhibited significant promotion of wound healing compared to the healing in controls without treatment. RT-PCR analysis of Wnt-1, -3a, -4, -5a, and -10 and β-catenin expression showed significantly increased expression in the ESWT group. The IHC staining showed that Wnt-3a and -5a and β-catenin levels were significantly increased in the ESWT and BIO treatment groups compared to the control groups. ESWT enhancement of diabetic wound healing is associated with modulation of the GSK-3β-mediated Wnt/β-catenin signaling pathway.

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“…Physical Parameters [19] The physical parameters of extracts from leaves of E. agallocha are shown in Table 4.…”

Section: Extractionmentioning

confidence: 99%

Untitled

Sharma¹

2019

IJGP

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Aim: The present study was designed to investigate the effect of different extracts of Excoecaria agallocha L. in the treatment of diabetes and its complications. Diabetes was induced by intraperitoneal administration of streptozotocin (65 mg/kg) for the development of diabetic nephropathy and neuropathy. Diabetic nephropathy was evaluated by assessing levels of fasting blood glucose, glycated hemoglobin (Hb A1C), serum insulin level, albuminuria, serum urea, uric acid, creatinine, and blood urea nitrogen along with altered lipid profile (total cholesterol, triglycerides, low-density lipoprotein [LDL], very LDL, and high-density lipoprotein-cholesterol). Materials and Methods: Oral treatment of diabetic rats with extracts of E. agallocha L. improved renal dysfunction indicated by a significant decrease in urinary albumin; serum creatinine, urea, and uric acid. Moreover, a significant increase in body weight along with a significant decrease in fasting blood glucose and Hb A1C in treated groups was observed. Hyperglycemia-induced oxidative stress in diabetic control rats indicated by a significant decrease in superoxide dismutase and reduced glutathione level with a significant increase in malondialdehyde (thiobarbituric acid reactive substances) levels was also ameliorated by treatment with extracts. Results and Discussion: Development of neuropathy was evident from marked hyperalgesia (thermal as well as mechanical) and tactile allodynia along with reduced Motor nervous conduction velocity (MNCV). An elevated level of nitrite, tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), and interleukin-1β (IL-1β) were also assessed in the sciatic nerve of diabetic neuropathy animals. Treatment with different extracts of E. agallocha L. significantly attenuated elevated thermal and mechanical hyperalgesia. Elevated tactile allodynia assessed using flexible Von Frey filaments was also reversed by extracts. Treatment with both extracts significantly attenuated the parameters of oxidative stress in sciatic nerve of diabetic neuropathy rats. Furthermore, level of nitrite, TNF-α, TGF-β, and IL-1β significantly increased in the sciatic nerve of diabetic neuropathy animals that were ameliorated by treatment with E. agallocha L. extracts. Conclusion: Histopathological changes in kidney, pancreas, liver, and sciatic nerve of diabetic rats were also reversed by the treatment. These findings suggested that treatment with E. agallocha L. ameliorated diabetes and its complications (diabetic nephropathy and diabetic neuropathic pain).

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Hyaluronic Acid–Povidone-Iodine Compound Facilitates Diabetic Wound Healing in a Streptozotocin-Induced Diabetes Rodent Model

Cited by 29 publications

References 24 publications

Supercritical Carbon Dioxide-decellularized Porcine Acellular Dermal Matrix combined with Autologous Adipose-derived Stem Cells: Its Role in Accelerated Diabetic Wound Healing

Supercritical Carbon Dioxide-decellularized Porcine Acellular Dermal Matrix combined with Autologous Adipose-derived Stem Cells: Its Role in Accelerated Diabetic Wound Healing

The Acceleration of Diabetic Wound Healing by Low-Intensity Extracorporeal Shockwave Involves in the GSK-3β Pathway

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