Hyaluronic acid modified mesoporous silica nanoparticles for targeted drug delivery to CD44-overexpressing cancer cells

Abstract

In this paper, a targeted drug delivery system has been developed based on hyaluronic acid (HA) modified mesoporous silica nanoparticles (MSNs). HA-MSNs possess a specific affinity to CD44 over-expressed on the surface of a specific cancer cell line, HCT-116 (human colon cancer cells). The cellular uptake performance of fluorescently labelled MSNs with and without HA modification has been evaluated by confocal microscopy and fluorescence-activated cell sorter (FACS) analysis. Compared to bare MSNs, HA-MSNs exhibit a higher cellular uptake via HA receptor mediated endocytosis. An anticancer drug, doxorubicin hydrochloride (Dox), has been loaded into MSNs and HA-MSNs as drug delivery vehicles. Dox loaded HA-MSNs show greater cytotoxicity to HCT-116 cells than free Dox and Dox-MSNs due to the enhanced cell internalization behavior of HA-MSNs. It is expected that HA-MSNs have a great potential in targeted delivery of anticancer drugs to CD44 over-expressing tumors.

Using hyaluronic acid-functionalized pH stimuli-responsive mesoporous silica nanoparticles for targeted delivery to CD44-overexpressing cancer cells

“…Doxorubicin hydrochloride (DOX), a cytotoxic drug that inhibits cellular mitochysis by interfering with the normal breakdown of microtubules during cell division, was chosen as the model drug. 3 This system, after being intravenously injected into the blood circulation, could deliver drugs into the tumor cells and avoid the clearance of the reticuloendothelial system, which could prolong the time of circulation and prevent the drug from being leaked. As for the prolonged blood circulation time, MSNs were accumulated in the tumor tissue via the enhanced permeation and retention effect, and by HA and CD44 receptors interacting, the nanoparticles would enrich in the target.…”

“…In recent decades, nanomaterials have been widely used in nanomedicine as potential diagnostic and therapeutic agents for cancer imaging and treatment, with less toxicity and improved efficacy. 3,4 Targeted delivery of more efficient cancer treatments has been well documented and is vital to distribute the drug throughout the body to minimize the side effects, which is desirable in order to increase the anticancer drug concentration at the target sites. 5 The major goal of chemotherapy is to ensure the safe and efficient transportation of drug molecules to the targeted sites.…”

Applications of nanotechnology for melanoma treatment, diagnosis, and theranostics

“…Silica can be made multiporous (which is then called mesoporous silica) to carry more drugs than as a simple sphere. 39 A layered double hydroxide nanoparticle is made by layering a hydroxyl (-OH) group on inorganic materials, to carry drugs. 40 This has been shown to increase immune response to a deoxyribonucleic acid (DNA) vaccine in a melanoma mouse model.…”

Synthesis and Characterization of Hyaluronic Acid Modified Colloidal Mesoporous Silica Nanoparticles

“…HA has been widely reported as a targeting ligand in biomedical field due to its excellent biocompatibility, biodegradability and targeting specificity [5]. Furthermore, HA acting as gatekeepers can be grafted onto various nanoparticles for controlled drugs release [6] since it can respond to hyaluronidase (HAase) which is found to be upregulated in many tumor matrices and endolysosomes [7].…”