Hyaluronic acid crosslinked with alginate hydrogel: A versatile and biocompatible bioink platform for tissue engineering

Thanh, Truc Nguyen; Laowattanatham, Navaporn; Ratanavaraporn, Juthamas; Sereemaspun, Amornpun; Yodmuang, Supansa

doi:10.1016/j.eurpolymj.2022.111027

Cited by 26 publications

(18 citation statements)

References 45 publications

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“…Similarly, it has been reported that the FTIR spectra of HA-Ty remain similar to the spectrum of HA after conjugating Ty on HA [54]. The FTIR spectra of amination HA-Ty showed a new peak assigned to amine groups of ethylenediamine at 3280cm -1 [29].…”

Section: Ftir Spectra Of the Hydrogelssupporting

confidence: 66%

“…Similarly, it has been reported that conjugation of ethylenediamine on HA changes the location of the protons of the sugar rings of HA and exhibits two new peaks before the sugar rings' peaks [29]. The percentage of 23.51% substitution degree was calculated by comparing the integration of the peaks related to ethylenediamine with the integration of the peak related to the methyl groups of HA as a reference peak with 1 H NMR.…”

Section: Nmr Analysismentioning

confidence: 98%

“…The substitution of carboxylic acid groups of HA-Ty with the primary amine of ethylenediamine was carried out following the previous methods [29,30]. Initially, the remaining carboxylic acid groups of HA were calculated by subtracting the substitution degree of Ty from the initial HA carboxylic acid groups via 1 H NMR of HA-Ty; then a 30-equivalent excess of ethylenediamine according to the remaining carboxylic acid groups was added into the aqueous solution of HA-Ty with a concentration of 0.5% w/v.…”

Section: Synthesis Of Ha-ty-nh2mentioning

confidence: 99%

See 2 more Smart Citations

Printable hyaluronic acid hydrogel functionalized with yeast-derived peptide for skin wound healing

Ghaffari-Bohlouli

Simińska-Stanny

Jafari

et al. 2023

International Journal of Biological Macromolecules

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Section: Ftir Spectra Of the Hydrogelssupporting

confidence: 66%

Section: Nmr Analysismentioning

confidence: 98%

Section: Synthesis Of Ha-ty-nh2mentioning

confidence: 99%

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Printable hyaluronic acid hydrogel functionalized with yeast-derived peptide for skin wound healing

Ghaffari-Bohlouli

Simińska-Stanny

Jafari

et al. 2023

International Journal of Biological Macromolecules

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“…PGA was added to the biomaterial ink immediately prior to printing to ensure that the enzyme was homogeneously mixed with the alginate. The thiol deprotection and concomitant disulfide cross-linking was slow enough for printing and sufficiently fast to retain the shape of the printed structures with reasonable resolution, similar to other alginate-based bioinks cross-linked using Ca 2+ . − However, the stiffness was not sufficient to allow for the printing of self-standing structures without a support hydrogel. We further evaluated the bioprinting of human primary fibroblast using the same strategy.…”

Section: Resultsmentioning

confidence: 99%

Enzymatically Triggered Deprotection and Cross-Linking of Thiolated Alginate-Based Bioinks

et al. 2022

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Thiolated polymers are widely used in hydrogels for drug delivery, tissue engineering, and biofabrication. The oxidation of thiols is spontaneous, resulting in the formation of disulfide bridges and cross-linking of polymers. The cross-linking process is, however, difficult to control and is initiated directly when the thiolated components are exposed to ambient conditions, which significantly complicates handling of the materials. Here, we show a fully bioorthogonal enzyme-mediated thiol-based chemistry for dynamic covalent cross-linking of carbohydrate-based hydrogels that circumvents the problems with uncontrolled thiol oxidation. Alginate was modified with cysteine residues, protected by an enzyme-labile thiol-protecting group (Phacm). Releasing the Phacm group by penicillin G acylase generates free thiols that oxidize under physiological conditions, resulting in a reversible cross-linking and formation of hydrogels with tunable stiffness. Prior to deprotection, the components can be exposed to ambient conditions. The enzyme-triggered deprotection and subsequent gelation allows for encapsulation of cells and 3D bioprinting of cell-laden hydrogel structures. Remaining deprotected thiols enabled postprinting modifications and hydrogel self-healing. The proposed hydrogel synthesis strategy significantly increases the versatility of thiol-based cross-linking chemistries and provides new possibilities to generate dynamic covalent hydrogels for a broad range of biomedical applications.

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“…Alginate is a hydrocolloid obtained from brown algae (Phaeophytes) and this polysaccharide comprises β‐D‐mannuronic acid and α‐D‐guluronic acid through a 1→4 linkage (Thanh et al, 2022). The carboxylic groups in the structure are negated by cations such as calcium, sodium, potassium, and so forth, forming alginate salts, including sodium and potassium alginate.…”

Section: Application and Characteristics Of Bnc‐based Packagingmentioning

confidence: 99%

Bionanocomposites for active and smart food packaging: A review on its application, safety, and health aspects

Kishore

Aravind

Singh

2023

J Food Process Engineering

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In recent years, the concept of plastic replacement and its optimal solution has commenced in the food-packaging sector. In that line, integrating nanotechnology with food packaging to improve material properties, aligned with the concept of active and intelligent packaging, has attracted consumer and industry attention. This review outlines the basics of bionanocomposites (BNCs), processing techniques, and their corresponding functions in innovative packaging techniques. The barrier, thermal, mechanical, and functional properties of film and their application in different food products have been summarized. In addition, the migration properties, safety, and health aspects of the latest findings of various BNC films have been discussed. It has been observed that the addition of fillers in BNC matrix improves the overall physical and functional properties of the film. In addition, BNC is used as a smart indicator to signify the product quality during transportation and storage. However, this review connotes the need for more research on migration and health aspects of the human body and implied the requirement to select BNC for specific food matrices. Practical ApplicationThe effective action of nanotechnology in the field of food packaging was attempted to improve the material properties, aligned with the concept of active and intelligent packaging. The inclusion of nano-sized particles into the organic matrices widely influenced its barrier, thermal, mechanical, and functional properties of the film.BNCs are also used as a smart indicator to indicate product quality throughout the storage and transportation. This article summarizes the basics of BNC, their processing techniques and functions in innovative packaging techniques, and their application in different food products. In addition, the migration properties, safety, and health aspects of the latest findings of various BNC films have been discussed in detail. This study will help researchers and industry experts to select the BNC for specific food matrices to improve the quality and safety of food product and human health.

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Hyaluronic acid crosslinked with alginate hydrogel: A versatile and biocompatible bioink platform for tissue engineering

Cited by 26 publications

References 45 publications

Printable hyaluronic acid hydrogel functionalized with yeast-derived peptide for skin wound healing

Printable hyaluronic acid hydrogel functionalized with yeast-derived peptide for skin wound healing

Enzymatically Triggered Deprotection and Cross-Linking of Thiolated Alginate-Based Bioinks

Bionanocomposites for active and smart food packaging: A review on its application, safety, and health aspects

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