Hyaluronic acid combined with mannitol to improve protection against free-radical endothelial damage: Experimental Model

Belda, José I; Artola, Alberto; García‐Manzanares, Maria; Ferrer, Consuelo; Haroun, Hazem Effat; Hassanein, A. M.; Baeyens, Vincent; Muñoz, Gonzalo; Alió, Jorge L.

doi:10.1016/j.jcrs.2004.11.055

Cited by 27 publications

(22 citation statements)

References 13 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SH is the main component of the ground substance of the connective eye tissues; it is already widely used alone in most of the commercial OVDs because of its capability of protecting against free-radical endothelial damage [22], and to its welldocumented mucoadhesive properties [23][24][25][26]. Chitosan thanks to its mucoadhesive properties has been proposed for topical use in ophthalmology and in mucosal sitespecific systems [11][12][13].…”

Section: Article In Pressmentioning

confidence: 99%

Novel polysaccharides-based viscoelastic formulations for ophthalmic surgery: Rheological characterization

Maltese¹,

Borzacchiello

Mayol

et al. 2006

Biomaterials

View full text Add to dashboard Cite

Section: Article In Pressmentioning

confidence: 99%

Novel polysaccharides-based viscoelastic formulations for ophthalmic surgery: Rheological characterization

Maltese¹,

Borzacchiello

Mayol

et al. 2006

Biomaterials

View full text Add to dashboard Cite

“…Addition of an antioxidant to HA has already been studied also in ophthalmology. In an animal model of oxidative stress using hydrogen peroxide (H 2 O 2 ), Belda et al [25] showed that HA associated with a low concentration of mannitol (0.5%) better protected the corneal endothelium, than HA alone.…”

Section: Introductionmentioning

confidence: 99%

Effect of Mannitol on Hyaluronic Acid Stability in Two in Vitro Models of Oxidative Stress

Rinaudo¹,

Lardy

Grange

et al. 2014

Polymers

View full text Add to dashboard Cite

Abstract:In this paper, we propose the evaluation of the mannitol's ability to reduce hyaluronic acid (HA) degradation using two different models of oxidative stress. Firstly, a solution of hyaluronan and a solution of the same HA including mannitol in PBS buffer were submitted to an oxidative stress generated by the addition of xanthine + xanthine oxidase generating oxygen free radicals. Different enzyme concentrations were used and the HA properties were studied after 24 h of contact at ambient temperature. Decreases of the viscosity of the solution were assessed by rheometry (viscous and elastic module) and that of HA molecular weight was determined by steric exclusion chromatography. Rheologic behavior was assessed on identical HA solutions subjected to another model of oxidative stress imposed by addition of hydrogen peroxide. The influence of mannitol concentration on HA degradation was also demonstrated. Whatever the stress applied, it appears very clearly that mannitol protects hyaluronic acid from mediated oxygen free radicals degradation. These in vitro results suggest that mannitol could be a simple way to significantly increase the intra-articular residence time of the injected hyaluronic acid and therefore might improve viscosupplementation effectiveness.

show abstract

“…The presence of other free radical scavengers in the OVDs (mannitol in Visiol and chondroitine sulphate in Viscoat) does not prevent MDA formation in this in vitro experiment. In a different study [26] , the authors demonstrated in vivo that OVDs containing sodium hyaluronate efficiently protected the endothelial cells against oxidative damage (H 2 O 2 ) in an experimental model with rabbits. The OVDs with higher concentrations of sodium hyaluronate (Visiol and Viscoat) achieved better endothelial protection.…”

Section: Discussionmentioning

confidence: 99%

“…Sodium-hyaluronate-based products have already been shown to have a free radical (like hydroxyl radicals or peroxynitrite radicals) scavenging effect [26][27][28] . However, the scavenging reaction on sodium hyaluronate is followed by a breakdown of the molecule.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Viscoelastics against Retina Lipid Peroxidation

Miranda

Bosch‐Morell²,

Belda³

et al. 2007

Ophthalmic Res

View full text Add to dashboard Cite

Viscoelastics or ophthalmic viscosurgical devices are routinely used during anterior segment surgery and also in posterior segment surgery. Studies of the harmful effects of phacoemulsification on corneal endothelial cells suggest that much of this damage is mediated by free radicals. In this study, we compare the possible effects against lipid peroxidation in the retina of three different viscoelastic substances: Viscoat, Healon and Visiol. Herein we demonstrate for the first time that viscoelastics are effective to protect the retina against lipid peroxidation, as can be seen by the slight increase of malondialdehyde in the homogenates incubated with viscoelastic exposed to light and to a temperature of 37°C when compared with the control homogenates.

show abstract

Hyaluronic acid combined with mannitol to improve protection against free-radical endothelial damage: Experimental Model

Abstract: All of the OVDs tested efficiently reduced endothelial lesions against free radicals compared with the control group in which no OVD was used. The following sequence for the efficacy of endothelial cell protection was established: Visiol>Viscoat>Healon>no OVD.

Cited by 27 publications

References 13 publications

Novel polysaccharides-based viscoelastic formulations for ophthalmic surgery: Rheological characterization

Novel polysaccharides-based viscoelastic formulations for ophthalmic surgery: Rheological characterization

Effect of Mannitol on Hyaluronic Acid Stability in Two in Vitro Models of Oxidative Stress

Protective Effect of Viscoelastics against Retina Lipid Peroxidation

Contact Info

Product

Resources

About