Hyaluronic Acid/Chitosan-g-Poly(ethylene glycol) Nanoparticles for Gene Therapy: An Application for pDNA and siRNA Delivery

Raviña, Manuela; Cubillo, Eva; Olmeda, David; Novoa-Carballal, Ramón; Fernández-Megía, Eduardo; Riguera, Ricardo; Sánchez, Alejandro; Cano, Amparo; Alonso, Marı́a José

doi:10.1007/s11095-010-0263-y

Cited by 85 publications

(43 citation statements)

References 42 publications

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“…A viable non-viral vector should be able to protect genes from complex biological environment, improve cellular uptake and facilitate endosomal escape. Due to the anionic nature of genes, various synthetic cationic polymers, such as poly(ethyleneimine) (PEI), [51] poly(amido amine) (PAA), [52] poly(β-amino ester) (PAE), [53] polypeptide, [54] poly(dimethylaminoethyl methacrylate) (PDMAEMA) [55] and some natural polymers, such as chitosan [56] and cationic starches [57] have been studied and used to form complex with genes through electrostatic interactions to fabricate gene delivery systems.…”

Section: Co-delivery Of Chemical Drugs and Genesmentioning

confidence: 99%

Co‐Delivery of Drugs and Genes Using Polymeric Nanoparticles for Synergistic Cancer Therapeutic Effects

Thambi

Lee

2017

Adv Healthcare Materials

103

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Drug and gene delivery systems based on nanoparticles, microparticles and hydrogels have been widely studied for cancer treatment in the past decade. To achieve an efficient and safe delivery, selection of drug and gene delivery carrier is critical. Biocompatible polymeric nanoparticles are considerably promising carrier candidates in delivery of drugs and genes because of their unique chemical and physical properties. However, delivery of a drug or gene sometimes cannot achieve a satisfactory treatment effect. Therefore, co‐delivery of dual drugs or co‐delivery of a drug and a gene in a polymeric nanoparticle has attracted attention. Such co‐delivery systems can overcome multi‐drug resistance of chemical drugs and achieve a synergistic therapeutic effect. In this progress report, we summarize recent progress in the preparation and application of polymeric drug and gene co‐delivery nanosystems. The remaining challenges and future trends in this field are also included.

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Section: Co-delivery Of Chemical Drugs and Genesmentioning

confidence: 99%

Co‐Delivery of Drugs and Genes Using Polymeric Nanoparticles for Synergistic Cancer Therapeutic Effects

Thambi

Lee

2017

Adv Healthcare Materials

103

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“…Furthermore, a ternary complex of nucleic acid/chitosan-g-poly(ethylene glycol) (CS-g-PEG)/HA was an effective carrier for delivery of both siRNA and pDNA, eliciting a biological response with minimal cytotoxicity [27]. Preparation of anionic polymer-coated polyplexes.…”

Section: Polyplexes and Lipoplexes Modifi Ed With Hyaluronic Acidmentioning

confidence: 99%

Progress in the development of Lipoplex and Polyplex modified with Anionic Polymer for efficient Gene Delivery

Hattori¹

2017

J Genet Med Gene Ther

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Nucleic acid-based therapy has become an increasingly important strategy for treating a variety of human diseases. In systemic therapy, a therapeutic gene must be delivered effi ciently to its target tissues without side effects. To deliver a therapeutic gene such as plasmid DNA (pDNA) or small interfering RNA (siRNA) to target tissues by systemic administration, cationic carriers such as cationic liposomes and polymers have been commonly used as a non-viral vector. However, the binary complex of therapeutic gene and cationic carrier must be stabilized in the blood circulation by avoiding agglutination with blood components, because electrostatic interactions between positively charged complexes and negatively charged erythrocytes can cause agglutination, and the agglutinates contribute to high entrapment of the therapeutic genes in the highly extended lung capillaries. One promising approach for overcoming this problem is modifi cation of the surface of cationic complexes with anionic biodegradable polymers such as hyaluronic acid, chondroitin sulfate, or polyglutamic acid. As another approach, we recently developed a sequential injection method of anionic polymer and cationic liposome/therapeutic gene complex (cationic lipoplex) for delivery of a therapeutic gene into the liver or liver metastasis. In this review, we describe recent advances in the delivery of therapeutic genes by lipid-and polymerbased carrier systems using anionic polymers.

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“…However, HA homopolymer is too weak and fluid to create a supportive scaffold. To overcome this limitation, HA is cross-linked with ethyl esters, benzyl esters, or other biodegradable polymers to enhance the mechanical properties while retaining excellent biocompatibility [368,369]. Such HA hydrogels are extremely versatile and can be fabricated into sheets, membranes, sponges, tubes, fibers, and scaffolds for wound healing [370], regeneration of the trachea [371], cartilage [372], vasculature [373], and nerve tissues [374].…”

Section: Proteins and Poly(amino Acids)mentioning

confidence: 99%

Biodegradable polymers for dental tissue engineering and regeneration

Conte¹,

Riccitiello²,

Luise³

et al. 2017

Biodegradable Polymers: Recent Developments and New Perspectives

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Hyaluronic Acid/Chitosan-g-Poly(ethylene glycol) Nanoparticles for Gene Therapy: An Application for pDNA and siRNA Delivery

Abstract: HA/CS-g-PEG nanoparticles can be easily modulated for the delivery of different types of gene molecules, offering great potential for gene therapy applications, as evidenced by their biological performance.

Cited by 85 publications

References 42 publications

Co‐Delivery of Drugs and Genes Using Polymeric Nanoparticles for Synergistic Cancer Therapeutic Effects

Co‐Delivery of Drugs and Genes Using Polymeric Nanoparticles for Synergistic Cancer Therapeutic Effects

Progress in the development of Lipoplex and Polyplex modified with Anionic Polymer for efficient Gene Delivery

Biodegradable polymers for dental tissue engineering and regeneration

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