Hyaluronate‐alginate gel as a novel biomaterial: Mechanical properties and formation mechanism

Oerther, Sandra; Le Gall, Hervé; Payan, Elisabeth; Lapicque, François; Presle, Nathalie; Hubert, Patrick; Dexheimer, Jean; Netter, Patrick; Lapicque, Françoise

doi:10.1002/(sici)1097-0290(19990420)63:2<206::aid-bit9>3.3.co;2-#

Cited by 13 publications

(19 citation statements)

References 19 publications

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“…The stimulatory effect of hyaluronate could be explained by both a physicochemical process influencing cellular activity and a biologic effect, already described in the literature for hyaluronate or its oligosaccharides. In fact, in the presence of hyaluronate, the resulting less tight network30 provides more free room for the formation of a new tissue via cell proliferation and extracellular matrix synthesis. However, the diffusion of nutrients is made easier.…”

Section: Discussionsupporting

confidence: 55%

“…Alginate sponges, with or without HA, exhibit a macroporous and interconnected network comprising numerous pores and channels. In opposition with observations performed on beads,30 the addition of HA to alginate does not increase the pore size of the sponges. Several phenomena could explain the macroporous structural organization of these sponges.…”

Section: Discussionsupporting

confidence: 52%

“…The samples were washed with distilled water and then dehydrated using acetone/water mixtures of increasing acetone concentration (20, 40, 60, 80, 95, and 100% acetone/water). After drying, the samples were treated with increasing concentrations of Epon 812 resin in acetone, as described by Oerther et al30 Samples were cut in 0.1‐μm‐thick sections with a diamond knife and observed in TEM.…”

Section: Methodsmentioning

confidence: 99%

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Sodium alginate sponges with or without sodium hyaluronate:In vitro engineering of cartilage

Miralles

Baudoin

Dumas

et al. 2001

J. Biomed. Mater. Res.

123

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Studies are underway to design biosystems containing embedded chondrocytes to fill osteochondral defects and to produce a tissue close to native cartilage. In the present report, a new alginate three-dimensional support for chondrocyte culture is described. A sodium alginate solution, with or without hyaluronic acid (HA), was freeze-dried to obtain large-porosity sponges. This formulation was compared with a hydrogel of the same composition. In the sponge formulation, macroscopic and microscopic studies demonstrated the formation of a macroporous network (average pore size, 174 microm) associated with a microporous one (average pore size, 250 nm). Histological and biochemical studies showed that, when loaded with HA, the sponge provides an adapted environment for proteoglycan and collagen synthesis by chondrocytes. Cytoskeleton organization was studied by three-dimensional fluorescence microscopy (CellScan EPR). Chondrocytes exhibit a marked spherical shape with a nonoriented and sparse actin microfilament network. Type II collagen was detected in both types of sponges (with or without HA) using immunohistochemistry. In conclusion, the sponge formulation affords new perspectives with respect to the in vitro production of "artificial" cartilage. Furthermore, the presence of hyaluronate within the alginate sponge mimics a functional environment, suitable for the production by embedded chondrocytes of an extracellular matrix.

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Section: Discussionsupporting

confidence: 55%

Section: Discussionsupporting

confidence: 52%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Sodium alginate sponges with or without sodium hyaluronate:In vitro engineering of cartilage

Miralles

Baudoin

Dumas

et al. 2001

J. Biomed. Mater. Res.

123

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“…In these systems, either acellular aqueous solutions of polymer or solutions containing desired cell type(s) are introduced at the tissue site. Subsequent gelation can occur by taking advantage of environmental differences between the polymeric solution and the in vivo environment such as temperature (14,15), ionic strength (16,17), or enzymatic activity (18). Alternatively, in vivo gelation can be accomplished by initiating the cross-linking of photopolymerizing polymer precursors by using cytocompatible photoinitiators (19,20).…”

mentioning

confidence: 99%

Controlling hydrogelation kinetics by peptide design for three-dimensional encapsulation and injectable delivery of cells

Haines-Butterick¹,

Rajagopal²,

Branco³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

601

739

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A peptide-based hydrogelation strategy has been developed that allows homogenous encapsulation and subsequent delivery of C3H10t1/2 mesenchymal stem cells. Structure-based peptide design afforded MAX8, a 20-residue peptide that folds and selfassembles in response to DMEM resulting in mechanically rigid hydrogels. The folding and self-assembly kinetics of MAX8 have been tuned so that when hydrogelation is triggered in the presence of cells, the cells become homogeneously impregnated within the gel. A unique characteristic of these gel-cell constructs is that when an appropriate shear stress is applied, the hydrogel will shear-thin resulting in a low-viscosity gel. However, after the application of shear has stopped, the gel quickly resets and recovers its initial mechanical rigidity in a near quantitative fashion. This property allows gel/cell constructs to be delivered via syringe with precision to target sites. Homogenous cellular distribution and cell viability are unaffected by the shear thinning process and gel/cell constructs stay fixed at the point of introduction, suggesting that these gels may be useful for the delivery of cells to target biological sites in tissue regeneration efforts.hydrogel ͉ self-assembly ͉ stem cell

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“…4 The association of alginate with hyaluronate has been recently suggested to combine the gel forming and mechanical properties of the first polymer with the healing capacities of the second one. [5][6][7][8] It was shown that alginic acid gel promotes hyaluronic acid retention in the composite materials. 9 Alginate and hyaluronate provide mild conditions for the immobilisation of proteins, such as human haemoglobin.…”

Section: Introductionmentioning

confidence: 99%

Electrodeposition of biopolymer films containing haemoglobin

Pang

Zhitomirsky

2011

Surface Engineering

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Electrochemical method has been developed for the fabrication of alginic acid-haemoglobin and hyaluronic acid-haemoglobin films. The deposition yield was studied as a function of the haemoglobin concentration in the polymer solutions and deposition time. The results of deposition yield measurements and Fourier transform infrared spectroscopy showed the formation of composite films containing haemoglobin in alginic acid or hyaluronic acid matrix. The haemoglobin content in the composite films was varied by the variation of haemoglobin concentration in the solutions. Electron microscopy studies showed that the film thickness can be varied in the range of 0-50 mm by the variation of deposition time at a constant deposition voltage. The method enabled the formation of uniform films of controlled thickness. The films were deposited as monolayers or multilayers, containing individual alginic acid-haemoglobin and hyaluronic acid-haemoglobin layers. The proposed method can be used for the surface modification of biomedical implants and fabrication of thin films for biosensors.

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Hyaluronate‐alginate gel as a novel biomaterial: Mechanical properties and formation mechanism

Cited by 13 publications

References 19 publications

Sodium alginate sponges with or without sodium hyaluronate:In vitro engineering of cartilage

Sodium alginate sponges with or without sodium hyaluronate:In vitro engineering of cartilage

Controlling hydrogelation kinetics by peptide design for three-dimensional encapsulation and injectable delivery of cells

Electrodeposition of biopolymer films containing haemoglobin

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