Electrodeposition of biopolymer films containing haemoglobin

Abstract

Electrochemical method has been developed for the fabrication of alginic acid-haemoglobin and hyaluronic acid-haemoglobin films. The deposition yield was studied as a function of the haemoglobin concentration in the polymer solutions and deposition time. The results of deposition yield measurements and Fourier transform infrared spectroscopy showed the formation of composite films containing haemoglobin in alginic acid or hyaluronic acid matrix. The haemoglobin content in the composite films was varied by the variation of haemoglobin concentration in the solutions. Electron microscopy studies showed that the film thickness can be varied in the range of 0-50 mm by the variation of deposition time at a constant deposition voltage. The method enabled the formation of uniform films of controlled thickness. The films were deposited as monolayers or multilayers, containing individual alginic acid-haemoglobin and hyaluronic acid-haemoglobin layers. The proposed method can be used for the surface modification of biomedical implants and fabrication of thin films for biosensors.

Fusing Sensor Paradigms to Acquire Chemical Information: An Integrative Role for Smart Biopolymeric Hydrogels

“…First, is an anodic neutralization mechanism of Figure c that involves the electrochemical generation of a low pH, and the localized protonation of alginate that neutralizes this polysaccharide and induces its gelation as alginic acid (p K a ≈3.5). This mechanism has been reported for the co‐deposition of a biosensing enzyme, however the majority of interest in the anodic electrodeposition of alginic acid appears to be to create composite coatings for non‐sensor applications …”

Electrophoretic deposition of polymer and composite films

“…16 The goal of this investigation was the fabrication of PVPBM films and composite PVPBM-CeO 2 films by the electrophoretic deposition (EPD) method. 17,18 In this investigation, the feasibility of EPD of PVPBM and composite films has been demonstrated. CeO 2 is an important material for corrosion protection of metals and alloys.…”

Alginic Acid Polymer-Hydroxyapatite Composites for Bone Tissue Engineering

“…The development of EPD of ALGH films provided a platform for the fabrication of composite films containing proteins, enzymes and other functional materials in the matrix of ALGH [ 23 , 24 , 25 , 26 ].…”