Hyaluronan-surfactant interactions in physiological solution studied by tensiometry and fluorescence probe techniques

The change in excited-state proton transfer kinetics of 1-naphthol in micelles upon the binding of polymers: The influence of hyaluronan hydration

“…The hydration or water-retention capacity is probably one of the most important aspects of the biological functions of hyaluronan (Kučerík et al, 2011). Hyaluronan-cationic surfactant interactions have been described in many previous papers (Bjoerling, Hersloef-Bjoerling, & Stilbs, 1995;Halasová, Krouská, Mravec, & Pekař, 2011;Hersloef, Sundeloef, & Edsman, 1992;Thalberg & Lindman, 1989;Thalberg, Lindman, & Karlstroem, 1990) but, to date, no reports about the influence of hyaluronan hydration in hyaluronan-surfactant interaction have been reported. It is known that polyelectrolytes, such as polystyrenesulfonate or hyaluronan, form so-called "pearl necklace" aggregates (Bjoerling et al, 1995) with surfactants due to the induced aggregation of surfactant molecules on polymer chain above certain surfactant and polymer concentrations.…”

Uncommon Structures of Oppositely Charged Hyaluronan/Surfactant Assemblies under Physiological Conditions

“…agg,TTA TTA mic c head (14) where V c and V head are the volumes of the micellar core and hydrated head group, respectively, and N mic is the aggregation number of an individual micelle, as discussed before. Finally, we arrive at the overall expression of the model for the whole scattering curve depending only on the size (R agg ) and content (χ agg,TTA and α agg,TTA ) of the aggregate, as well as on the average number (K) of micelles within the cylindrical aggregates with an average distance (D) that is accounted for by P mic (K,D) (this model is described in detail in ref 65) for a given mixing ratio of HA/TTAB…”

“…A full discussion of HA lies beyond the scope of this work, but its properties are naturally most interesting when looking at mixtures with proteins , or other colloidal components, such as surfactants or lipids, , especially because the behavior in such more complex situations is relevant to its behavior in biological systems. Accordingly, HA was studied in various mixtures, e.g., in the presence of liposomes or proteins.…”

“…They showed that a large range of mixing ratios exists, where precipitation occurs (the two-phase region), and also that the addition of salt first dampens and then completely suppresses precipitation, thereby leading to homogeneous solutions (as they are also formed for an excess of either HA or TTAB). Pekař et al studied the interaction of HA with a wide range of oppositely charged surfactants , and showed that HA lowers the cytotoxicity of positively charged micelles, presumably simply by reducing the activity of cationic TTA surfactant. Light scattering experiments on polyelectrolyte–surfactant complexes (PESCs) of NaHA and C 10 TAB showed aggregates larger than 100 nm, and up to three distinguishable dynamical modes were deduced from a CONTIN analysis of dynamic light scattering (DLS) data …”

Gyógyszerhatóanyag hordozó rendszerek szerkezete és a hatóanyag leadás kinetikája

“…A természetes poliszacharidok funkciós csoportjai (karboxil, amino, hydroxyl) lehetőséget nyújtanak mind kémiai, mond biokémiai módosításokra, melyekkel számos származék előállíthatására nyílik lehetőség [75]. A természetes biomolekulákhoz hasonlóan, a poliszacharidok is hidrofil, nagy stabilitású, biokompatibilis és nem-toxikus anyagok [76,77]. A hialuronsav (HA) megtalálható a szövetekben, a testfolyadékban és a bőrben is [78], így a HA ideális alkotóeleme a kozmetikumoknak, az orvosi,-és a gyógyászati alkalmazásoknak is; a reumatológiában és az urológiában is sikeresen alkalmazott hatóanyag [79,80].…”