HVJ-liposome mediated gene transfer into hepatocytes in vivo

Hirano, Toshio; Fujimoto, Jiro; Ueki, Takahiro; Yamamoto, Hidenao; Takeuchi, Masaharu; Okamoto, Eizo; Takahashi, Hiroshi; Morisita, Ryuichi; Sawa, Yoshiki; Kaneda, Yasufumi

doi:10.1016/s0168-8278(98)80118-9

Cited by 15 publications

(9 citation statements)

References 19 publications

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“…Only minor changes were observed in the creatinine and LDH levels in both groups. previous results on rats (22). The difference in our findings may depend on the amount of DNA administered to each pig, or due to species specificity of the sialic receptor for sendai viral transfection.…”

Section: Discussioncontrasting

confidence: 71%

The Efficacy and Safety of Gene Transfer into the Porcine Liver In Vivo by HVJ (Sendai Virus) Liposome

Kawashita

Fujioka²,

Ohtsuru³

et al. 2005

Transplantation

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Section: Discussioncontrasting

confidence: 71%

The Efficacy and Safety of Gene Transfer into the Porcine Liver In Vivo by HVJ (Sendai Virus) Liposome

Kawashita

Fujioka²,

Ohtsuru³

et al. 2005

Transplantation

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“…Each type of delivery system has individual advantages and limitations (23). A fusigenic liposome with the hemagglutinating virus of Japan (HVJ-liposome) represents an attractive in vivo gene-delivery system (22) characterized by persistent gene expression and low cytotoxicity (17). We used this system in the present study to transduce HGF in mice developing IBD, previously having achieved stable HGF expression in a rat liver cirrhosis model, a rat obstructive nephropathy model, and a mouse graft vs. host disease model (13,26,42).…”

mentioning

confidence: 99%

Ameliorating effect of hepatocyte growth factor on inflammatory bowel disease in a murine model

Oh¹,

Iimuro²,

Takeuchi³

et al. 2005

American Journal of Physiology-Gastrointestinal and Liver Physi

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Hepatocyte growth factor (HGF), a multifunctional cytokine, accelerates intestinal epithelial proliferation. We studied the effects of HGF in mice with trinitrobenzene sulfonic acid-induced colitis, which shows clinical and molecular resemblance to Crohn's disease. Mice with colitis repeatedly were transfected intramuscularly with human HGF cDNA. Weight, survival, histopathology, proinflammatory cytokine mRNAs, and leukocyte infiltration were assessed. Treatment with HGF cDNA induced tyrosine phosphorylation of intestinal c-Met/HGF receptors, inhibited apoptosis, and promoted mitosis in intestinal epithelial cells, accelerating intestinal epithelial restoration and suppressing inflammation. Transfection with HGF cDNA markedly suppressed intestinal mRNA expression of T-helper 1 cytokines such as interleukin-12 and -1beta, interferon-gamma, and tumor necrosis factor-alpha. Numbers of total and CD4-positive T cells, neutrophils, and myloperoxidase activity in intestinal epithelium were diminished by HGF gene transfer, which also prevented weight loss, and improved survival. HGF might prove useful for controlling inflammatory bowel disease.

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“…However, taking into consideration that the surface area of the brain capillary endothelium is 100 -250 cm 2 /g brain (Takasato et al, 1986;Johnston et al, 1996), the concentration of HVJ-liposomes to which the brain capillary endothelium is exposed in vivo is estimated to be 200 -400 HAU/cm 2 , which was not cytotoxic to MBEC4 cells in vitro. HVJ-liposomes were also proved to be devoid of inflammatory effects and immunogenic activity even after repeated transfections (Hirano et al, 1998). We conclude that HVJ-liposomes at the concentration used in this study should be well tolerated for application to the brain capillary endothelium in vivo.…”

Section: Evaluation Of Cytotoxicitymentioning

confidence: 67%

Transluminal Gene Transfer into Brain Capillary Endothelial Cells In Vivo with HVJ-liposomes

Jiang

Matsuo

Koyabu

et al. 2002

Journal of Drug Targeting

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Bioactive proteins or peptides cannot be effectively delivered into brain capillary endothelial cells (BCECs) or brain parenchyma. In this study, we selectively transferred Escherichia coli beta-galactosidase gene (beta-gal) as a model gene into BCECs by using the hemagglutination virus of Japan (HVJ)-liposomes. HVJ-liposomes encapsulating a beta-gal plasmid were used to transfect MBEC4 cells in vitro, and were administrated via the internal carotid artery to rat in vivo. Success of the procedure was confirmed by the detection of 116 kDa beta-gal protein in transfected MBEC4 cells and in brain capillaries isolated from transfected rats, by Western blot analysis and histological staining. The enzymatic activities of beta-galactosidase were 5- to 10-fold and 20-fold higher than when beta-gal-containing liposomes without fusogenic activity (uncoated liposomes) or plasmid alone were employed in vitro and in vivo, respectively. Thus, HVJ-liposomes were demonstrated to be a useful vector to transfer a foreign gene into the brain capillary endothelium in vivo via the transluminal route.

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HVJ-liposome mediated gene transfer into hepatocytes in vivo

Cited by 15 publications

References 19 publications

The Efficacy and Safety of Gene Transfer into the Porcine Liver In Vivo by HVJ (Sendai Virus) Liposome

The Efficacy and Safety of Gene Transfer into the Porcine Liver In Vivo by HVJ (Sendai Virus) Liposome

Ameliorating effect of hepatocyte growth factor on inflammatory bowel disease in a murine model

Transluminal Gene Transfer into Brain Capillary Endothelial Cells In Vivo with HVJ-liposomes

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