Hungarian population data on six STR loci ? HUMVWFA31, HUMTH01, HUMCSF1PO, HUMFES/FPS, HUMTPOX, and HUMHPRTB ?derived using multiplex PCR amplification and manual typing

Abstract

We present a Hungarian population study for six tetrameric short tandem repeat (STR) loci employing multiplex PCR amplification, electrophoresis of the PCR products in DNA sequencing gels and subsequent detection of allelic fragments by silver staining. The loci were HUMVWFA31, HUMTH01, HUMCSF1PO, HUMFES/ FPS, HUMTPOX, and HUMHPRTB. All loci met Hardy-Weinberg expectations in the examined Hungarian Caucasian population sample (N = 223 individuals). In addition, there was no evidence for association of alleles among the five autosomal loci HUMVWFA31, HUMTH01, HUMCSF1PO, HUMFES/FPS, and HUMTPOX.

Forensic validation of a multiplex containing nine STRs - population genetics in Northern Poland

“…This allele was sequenced in both directions using the BigDye Terminator cycle sequencing kit and the sequence confirmed as (TTTC) 3 TTTT TTCT (CTTT) 8 CTCC (TTCC) 2 which corresponds to the FGA allele 16 which had not previously been found in the Polish population. The sequence is consistent with the sequence of repeat region of shorter FGA alleles reported by Barber et al [13] Comparison of the precision of the allele size determination for the Profiler Plus with the application of the internal standards GS 500 and the FL-CXR 60-400 bp Some precision problems during analysis of FGA alleles on the ABI310 sequencer using the monoplex reaction were shown earlier [6].…”

“…The introduction of multiplex PCR techniques allowed simultaneous, rapid and robust amplification of several DNA loci from minute biological stains or fresh blood [2,3,4]. One of the commercially available multiplex kits is the Profiler Plus from Perkin-Elmer (PE) consisting of the nine polymorphic loci D3S1358, VWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317 and D7S820 and amelogenin.…”

A population study of the STR loci HUMLPL, HUMF13B, and HUMF13A01 in Hungary

“…There was no evidence to suggest departures from independence either within or between the STR loci based on the exact test Furthermore, using the s k 2 criterion [8] there was no evidence for allelic association among the three STR loci (s k 2 = 0.651, 95% confidence interval of variance is 0.512-0.717). Additionally, based on the interclass correlation test no evidence was found for departures from the expectation of independence between any of the three STR and other previously reported [3] microsatellite polymorphisms (HUMVWFA31, HUMFES/FPS, HUMTH01, HUMTPOX, HUMCSF1PO; P ≥ 0.137). The forensic efficiency data (Table 1) suggest that the three STR loci investigated are very discriminating in the Hungarian population (combined discrimination power = 0.998, combined mean exclusion chance = 0.858)…”

“…In spite of the increasing number of Hungarian population databases [1][2][3], there are many forensically interesting STR polymorphisms which have not yet been surveyed in Hungary. This paper presents allele frequency distributions and locus independence data for the three STR loci HUMLPL, HUMF13B, and HUMF13A01 [4] in a Hungarian population sample.…”

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

“…Despite the complex, hypervariable motifs of some tetramer loci-posing a challenge for appropriate genotyping, among the types of STR markers-the tetranucleotide repetition has been developed for common forensic applications [9, [125][126][127]. Due to the narrow allele size range of STRs, the monoplex form of PCR has been rapidly replaced by the quadruplex form [9, [128][129][130][131]. The increasing number of standardized STR loci and fluorophore molecules combined with capillary electrophoretic separation [90,97,132,133] has established the standard sets of STR markers for the forensic community [9].…”