Humoral immune response in mice immunised by oral and subcutaneous routes with Isosoy proteins

Abstract

This study compares the allergenic potential of Isosoy † , a soy germen commercialised as isoflavone for dietary supplements, with crude soy extract (CSE) and purified isoflavone. Specific antibodies (IgG, IgE and IgG1) generated in Swiss mice by oral and subcutaneous route with Isosoy † also recognised proteins of crude soy extract, but no reaction against purified isoflavone, like clearly demonstrated by ELISA, Western blot and immunoprecipitation assay. These results are in agreement with the same protein profile between Isosoy † and CSE observed by polyacrylamide gel electrophoresis (SDS-PAGE). The immunoprecipitation assay demonstrates that Isosoy † boiling cannot inactivate the interaction with specific antibodies generated by oral route with unboiled Isosoy † . Results suggest that Isosoy † could lead to allergic reactions even after heat treatment.

Humoral immune response in mice immunized by oral route with phaseolamine extracted from common bean (Phaseolus vulgaris)

“…The ELISA test of the sample used in the present study corroborates with the results reported by Santos et al (2012) who found similar results evaluating the immunological response of mice immunized with a protein present in a functional food, such as phaseolamine. They used the serum samples obtained from the mice sensitized, it was observed that the protein was able to induce the synthesis of IgG immunoglobulins by subcutaneous and oral routes and it did not induce the synthesis of IgE and IgG1 immunoglobulins.…”

“…According to Santos et al (2012) in the western blotting test, using the polyclonal antibodies obtained by the subcutaneous route, it was demonstrated that the isosoy® proteins, present in a functional food similar to phaseolamine, only induced the formation of type IgG immunoglobulins and IgG1 and IgE were not detected, corroborating with the results found in our study, suggesting that the product is immunologically safe for use. Although positive results were found in this research, there are still few studies related to phaseolamine and its use as a pharmacological tool.…”

Immunological response in mice immunised via oral route with açaí (Euterpe oleraceaMart.)

“…The results of this study indicate that the açaí berry can cause allergic reactions, since the IgE and some isotypes of IgG, especially IgG1 and IgG2a, are involved in allergic processes (Paschoal, 2007;Santos et al, 2012). The increase of the production of IgG1 ( Figure 2C) suggests the occurrence of a modulation of the immune response to the profile Th2, indicating the probable deflagration of allergic processes.…”

“…The precipitate was resuspended in 20 mM of Tris-HCl buffer, pH 7.5, containing 1% of Triton X-100 (v/v) and 0.5 M NaCl, and then centrifuged (9800 x g for 5 min). In order to eliminate the Triton, the precipitate was washed three times with 10 mM phosphate buffer, pH 7.2 with 0.1% of SDS (m/v) (Santos et al, 2012).…”

Humoral immune response in mice immunized orally with conventional and transgenic soy extracts (Glycine max)

“…According to Santos et al (2012) in the western blotting test, using the polyclonal antibodies obtained by the subcutaneous sensitization, it was demonstrated that the Isosoy® proteins, present in a functional food similar to soybean, only induced the formation of type IgG immunoglobulins. Furthermore, the authors did not detect IgG1 and IgE in serum, diverging with the results found in the present study, suggesting that soy consumption should be cautious, due to its allergenic potential.…”