Humoral immune response against the S-antigen/TNFα common epitope in rat EAU suppressed by the monoclonal antibody S2D2

Anti-retinal autoantibodies in experimental ocular and systemic toxoplasmosis

“…The time-lapse that was required for the production of antibodies was 10 to 15 days (Fig. 2b and c), which is consistent with previously published data [33]. In infectionimmunized animals, intravitreal reinfection with Toxoplasma did not induce any change in the serum antibody levels, which is in accordance with the situation in humans.…”

“…Using an experimental rabbit model of systemic and ocular toxoplasmosis [33], and an established autoantibody ELISA technique [32,33], we demonstrated that the concentrations of anti-retinal antibodies increased in the infected eyes during the early post-infection period. The data correlated with the degree of infectious tissue destruction at the clinical level (data not presented).…”

“…The level of antibodies against the retinal S-antigen and peptide 35 were similarly quantified using the enzymelinked immunosorbent assay (ELISA) technique [32,33]. and since there is a sequence homology between peptide 35 from rabbit, rat and human, we checked the antibody response to both, the entire bovine S-Ag molecule and to peptide 35 in toxoplasmic rabbits.…”

“…By drawing on an experimental rabbit model of systemic and primary and secondary ocular toxoplasmosis [30], we wished to investigate the onset and development of the humoral response to the retinal S-antigen, using an established autoantibody ELISA technique [31][32][33]. A comparison between primary ocular and systemic toxoplasmosis will reveal whether any cross-reactivity exists between toxoplasmal and retinal antigens, and that between primary and secondary OT will disclose whether a correlation exists between the levels of anti-retinal antibodies and the degree of ocular tissue destruction.…”