Humoral immune parameters of cultured Atlantic halibut (Hippoglossus hippoglossus L.)

Lange, Sigrun; Guðmundsdóttir, Bjarnheiður K.; Magnadóttir, Bergljót

doi:10.1006/fsim.2000.0333

Cited by 106 publications

(61 citation statements)

References 30 publications

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“…The turbidimetric assay for lysozyme activity was carried out according to Lange et al (2001) with minor modifications. Thus, plasma (50 µL) was added to 2 mL of a suspension of Micrococcus lysodeikticus (Actinobacteria: Micrococcaceae) (0.2 mg mL -1 ) in a 0.05 M sodium phosphate buffer (pH 6.2).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Immunomodulatory Effects of Silymarin Extract (Silybum Marianum) on Some Immune Parameters of Rainbow Trout, Oncorhynchus Mykiss (Actinopterygii: Salmoniformes: Salmonidae)

Ahmadi¹,

Banaee²,

Vosoghei³

et al. 2012

Acta Icth et Piscat

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Ataeimehr B. 2012. Evaluation of the immunomodulatory effects of silymarin extract (Silybum marianum) on some immune parameters of rainbow trout, Oncorhynchus mykiss (Actinopterygii: Salmoniformes: Salmonidae). Acta Ichthyol. Piscat. 42 (2): 113-120.Background. Herbal medicines are increasingly used for their effects on the immune system. Silymarin, a mixture of flavonolignans from the seed of milk thistle (Silybum marianum), is used in treatment of liver disease, food and drug poising, and foetal diseases such as viral hepatitis, diabetes, and ischemia. Although, immunostimulant effects of the dietary silymarin were studied on different experimental animals, no information is available about the effects of silymarin on immune parameters of fish. The presently reported study was conducted to investigate the immunomodulatory effects of silymarin on some immunological and haematological parameters of rainbow trout. Materials and methods. Juvenile rainbow trout, Oncorhynchus mykiss (Walbaum, 1792), were maintained in 1000 L fiberglass tanks at 15 ± 2ºC supplied with systems of water recirculation and aeration. Silymarin extract incorporated into diets (0.0, 0.1, 0.4, and 0.8 g of per 1 kg of feed) of fish. The trout were fed silymarin-supplemented diet for 30 days. Haematological parameters, such as red blood cell count (RBC), white blood cell count (WBC), haematocrit (Hct), haemoglobin (Hb), differential leukocyte-and immunological parameters such as peroxidase, lysozyme, and complement activities, total protein, albumin and globulin levels were measured on day 7, 15, and 30 days of silymarin treatment. Results. The results indicated that oral administration of silymarin in fish, after 15 and 30 days of experimental periods, might enhance haematological and immunological parameters including lysozyme and complement activities, total protein and globulin levels, compared to the controls. Conclusion. The results suggest that oral administration of silymarin may be useful to strengthen the immune system in rainbow trout.

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Section: Methodsmentioning

confidence: 99%

Evaluation of the Immunomodulatory Effects of Silymarin Extract (Silybum Marianum) on Some Immune Parameters of Rainbow Trout, Oncorhynchus Mykiss (Actinopterygii: Salmoniformes: Salmonidae)

Ahmadi¹,

Banaee²,

Vosoghei³

et al. 2012

Acta Icth et Piscat

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“…The lysozyme activity assay was followed by the previously described method [38] with some modifications [34,39]. Thus, 100 ml of serum or mucus supernatant was diluted in four twofold serial dilutions in 0.05 M SPB at pH 6.2 in 96-well microtitre flat bottom plates.…”

Section: Lysozyme Activity Of Serum and Gut Mucusmentioning

confidence: 99%

Innate immune responses in rainbow trout (Oncorhynchus mykiss, Walbaum) induced by probiotics

Kim

Austin

2006

Fish & Shellfish Immunology

311

192

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“…Compared with the values of the standards from the kits, C3 and C4 contents were expressed in μg ml -1 [22]. The turbidimetric assay for lysozyme activity was carried out according to [23] with minor modifications. Thus, plasma (50 µL) was added to 2 mL of a suspension of Micrococcus luteus (Actinobacteria: Micrococcaceae) (0.2 mg mL -1 ) in a .05 M sodium phosphate buffer (pH 6.2).…”

Section: Methodsmentioning

confidence: 99%

Effects of Aflatoxin-Contaminated Feed on Immunological Parameters of Common Carp (Cyprinus Carpio)

Bitsayah¹,

Banaee²,

Haghi³

2018

IJT

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Humoral immune parameters of cultured Atlantic halibut (Hippoglossus hippoglossus L.)

Cited by 106 publications

References 30 publications

Evaluation of the Immunomodulatory Effects of Silymarin Extract (<I>Silybum Marianum</I>) on Some Immune Parameters of Rainbow Trout, <I>Oncorhynchus Mykiss</I> (Actinopterygii: Salmoniformes: Salmonidae)

Evaluation of the Immunomodulatory Effects of Silymarin Extract (<I>Silybum Marianum</I>) on Some Immune Parameters of Rainbow Trout, <I>Oncorhynchus Mykiss</I> (Actinopterygii: Salmoniformes: Salmonidae)

Innate immune responses in rainbow trout (Oncorhynchus mykiss, Walbaum) induced by probiotics

Effects of Aflatoxin-Contaminated Feed on Immunological Parameters of Common Carp (Cyprinus Carpio)

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