Human zygote morphological indicators of higher rate of arrest at the first cleavage stage

Zamora, Raquel Blanes; Sánchez, Rebeca Vaca; Pérez, José Antonio González; Díaz, R Rodríguez; Quintana, Delia Báez; Bethencourt, José Carlos Alberto

doi:10.1017/s0967199410000407

Cited by 20 publications

(10 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Specifically, our data confirm the efficacy of the pronuclear morphology evaluation to predict the clinical pregnancy [4-11,21]. At this regard, current findings highlight that the pronuclear morphology is an important tool not only to individuate abnormal fertilizations (only 1 PN or more than 2 PN) but to select zygotes when ET is performed at day 1, as recently recommended by the European Society of Human Reproduction and Embryology (ESHRE) [1].…”

Section: Discussionsupporting

confidence: 82%

“…Previous data have suggested that zygote-score could be an efficient tool for embryo selection if combined with embryo morphology evaluation on days 2 and 3 [10,11]. In particular, the morphological characteristics of the human zygote may suggest an increased risk of arrest of the embryo [11]. Moreover, to date, there is not definitive consensus about the clinical efficacy of the zygote pronuclear morphology.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pronuclear morphology evaluation in in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles: a retrospective clinical review

et al. 2013

View full text Add to dashboard Cite

BackgroundThe assessment of the embryo quality is crucial to maintain an high pregnancy rate and to reduce the risk of multiple pregnancy. The evaluation of the pronuclear and nucleolar characteristics of human zygote have been proposed as an indicator of embryo development and chromosomal complement. The aim of the current study was to assess the role of pronuclear morphology evaluation in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles.MethodsRetrospective clinical analysis on 755 non-elective transfers of only one embryo (ET). Embryo assessment was performed in days 1 and 2. Clinical and biological data were recorded and analyzed according to embryo and/or pronuclear morphology.ResultsBoth pronuclear and embryo morphology were significantly related to clinical pregnancy and live-birth rates. No significant difference in clinical pregnancy and live-birth rates was detected when the pronuclear and embryo morphology assessments were combined. Embryo morphology and maternal age were the only independent predictors of favorable outcome by logistic regression analysis.ConclusionsPronuclear evaluation is effective to select the best zygotes if ET is performed at day 1, whereas it did not improve the clinical outcomes when combined with embryo morphology evaluation in day 2.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Pronuclear morphology evaluation in in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles: a retrospective clinical review

et al. 2013

View full text Add to dashboard Cite

show abstract

“…PN and NPB morphologies were previously associated with aneuploidy. [38][39][40][41][42][43] Here we find that the morpho-dynamic properties as characterized by the trajectories of the PN'i and the NPB's differentiate between the potential of MC versus LB outcomes. The maximal distance between NPB pairs within the small PN ( 1 ) is greater in MC embryos than in LB embryos ( Fig.…”

Section: Feature Importancementioning

confidence: 61%

Embryo classification beyond pregnancy: Early prediction of first trimester miscarriage using machine learning

Amitai

Kan‐Tor

Srebnik

et al. 2020

Preprint

View full text Add to dashboard Cite

ObjectiveDevelop a machine learning classifier for predicting the risk of cleavage-stage embryos to undergo first trimester miscarriage based on time-lapse images of preimplantation development.DesignRetrospective study of a 4-year multi-center cohort of women undergoing intra-cytoplasmatic sperm injection (ICSI). The study included embryos with positive indication of clinical implantation based on gestational sac visualization either with first trimester miscarriage or live birth outcome. Miscarriage was determined based on negative fetal heartbeat indication during the first trimester.SettingData were recorded and obtained in hospital setting and research was performed in university setting.Patient(s)Data from 391 women who underwent fresh single or double embryo transfers were included.Intervention(s)None.Main Outcome Measure(s)A minimal subset of six non-redundant morphodynamic features were screened that maintain high prediction capacity. Using this feature subset, XGBoost and Random Forest models were trained following a 100-fold Monte-Carlo cross validation scheme. Feature importance was scored using the SHapley Additive exPlanations (SHAP) methodology. Miscarriage versus live-birth outcome prediction was evaluated using a non-contaminated balanced test set and quantified in terms of the area under the receiver operating characteristic (ROC) curve (AUC), precision-recall curve, positive predictive value (PPV), and confusion matrices.Result(s)Features that account for the distribution of the nucleolus precursor bodies within the small pronucleus and pronuclei dynamics were highly predictive of miscarriage outcome. AUC for miscarriage prediction of validation and test set embryos using both models was 0.68-to-0.69. Clinical utility was tested by setting two classification thresholds accounting for high sensitivity 0.73 with 0.6 specificity and high specificity 0.93 with 0.33 sensitivity.Conclusion(s)We report the development of a decision-support tool for identifying the embryos with high risk of miscarriage. Prioritizing embryos for transfer based on their predicted risk of miscarriage in combination with their predicted implantation potential will improve live-birth rates and shorten time-to-pregnancy.CapsuleThe risk of first trimester miscarriage of cleavage stage embryos is predicted with AUC 68% by screening a minimal subset of six non-redundant morpho-dynamic features and training a machine-learning classifier.

show abstract

“…In the second one, after the study of 1,894 zygotes, the Authors concluded that zygote score allows to select the most competent embryos to transfer [23]. More recently, Zamora et al [44] confirmed the correlation between NPBs and the cleaved embryos on day 2 in a prospective study conducted on 2,105 zygotes [44]. …”

Section: Resultsmentioning

confidence: 99%

Pronuclear morphology evaluation for fresh in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles: a systematic review

et al. 2013

View full text Add to dashboard Cite

The current systematic review was aimed to assess the effectiveness of the zygote morphology evaluation in fresh in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles. All available studies reporting on zygote morphology and clinical and/or biological outcomes were analyzed. Forty studies were included in the final analysis. Fourteen different zygote scoring systems were employed. Zygote morphology correlated significantly with embryo quality and cleavage, blastocyst stage, embryonic chromosome status, in a high proportion of the studies which assessed the specific outcome [15/25 (60%), 15/20 (75%), 7/8 (87.5%), 6/6 (100%), respectively]. On the other hand, only a reduced proportion of papers showed a statistically significant relationship between implantation, pregnancy and delivery/live-birth rates and zygote morphology score [12/23 (52.2%), 12/25 (48%), 1/4 (25%), respectively]. In conclusion, our findings demonstrate the lack of conclusive data on the clinical efficacy of the zygote morphology evaluation in fresh IVF/ICSI cycles, even if biological results showing a good relationship with embryo viability suggest a role in cycles in which the transfer/freezing is performed at day 1.

show abstract

Human zygote morphological indicators of higher rate of arrest at the first cleavage stage

Cited by 20 publications

References 24 publications

Pronuclear morphology evaluation in in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles: a retrospective clinical review

Pronuclear morphology evaluation in in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) cycles: a retrospective clinical review

Embryo classification beyond pregnancy: Early prediction of first trimester miscarriage using machine learning

Pronuclear morphology evaluation for fresh in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles: a systematic review

Contact Info

Product

Resources

About