Human umbilical cord-derived mesenchymal stem cells conditioned medium ameliorates CCl<sub>4</sub>-induced liver fibrosis through regulation of expression and activity of liver lysyl oxidase

Bahmani, Mahdi; Ziamajidi, Nasrin; Hashemnia, Mohammad; Abbasalipourkabir, Roghayeh

doi:10.1080/15569543.2020.1813779

Cited by 4 publications

(6 citation statements)

References 41 publications

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“…Intravenous Injection. Bahmani et al reported that intravenous injection of UMSC-CM (umbilical mesenchymal stem cells-condition media) could significantly improve liver fibrosis in male rats [38,39]. Tail vein injection of SCderived hepatocytes or SC-derived extracellular vesicles to animal with liver injuries had attenuated collagen deposition, liver fibrosis, and extracellular matrix alteration, it had decreased AST, ALT, increased serum ALB concentration, produced CK-18, enhanced liver regeneration and repair, and prolonged recipient survival [9,[40][41][42].…”

Section: In Vivo Results and Animalmentioning

confidence: 99%

“…In most of the in vivo studies, transplantation has been performed as a direct stem cells therapy (undifferentiated SCs) to different sites in patient or animals with liver disorders, whose significant restore or reduce of liver disorders and results of evaluating the special liver genes and protein expression or clinical manifestation show the efficacy of differentiation SCs into hepatocytes [ 1 , 9 , 14 , 21 , 25 , 38 – 44 , 47 – 49 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

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The Efficiency of Stem Cells (SCs) Differentiation into Functional Hepatocytes for Treating Liver Disorders: A Systematic Review

Nemati

Ebrahimi

Abolhassani

et al. 2023

BioMed Research International

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Stem cells provided new opportunity to treat various diseases, including liver disorders. Stem cells are unspecialized cells, stimulating influential research interest be indebted to their multipotent self-renewal capacity and differentiation characteristics into several specialized cell types. Many factors contribute to their differentiation into different cell types such as insulin producing cells, osteoblast, and hepatocytes. Accordingly, wide range methods and materials have been used to transform stem cells into hepatocytes, but effectiveness of differentiation is different and depends on several factors such as cell-to-cell adhesion, cell-to-cell contact, and cell biological change. Search was done in PubMed, Scopus, and WOS to evaluate results of studies about stem cells differentiation for higher efficacy. Among more than 28000 papers, 51 studies were considered eligible for more evaluations. Results indicated that most studies were performed on mesenchymal stem cells compared with other types. Acute liver failure was the most investigated liver disorder, and tissue engineering was the most investigated differentiation methods. Also, functional parameters were the most evaluated parameters in assessing differentiation efficacy. We summarize recent advances in increasing efficiency of stem cells differentiation using varied materials, since promising results of this review, further studies are needed to assess efficiency and safety of these cells transplantation in some liver disease treatment.

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Section: In Vivo Results and Animalmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Efficiency of Stem Cells (SCs) Differentiation into Functional Hepatocytes for Treating Liver Disorders: A Systematic Review

Nemati

Ebrahimi

Abolhassani

et al. 2023

BioMed Research International

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“…The culture, isolation, and purity evaluation of UC-MSC from human infant umbilical cord samples were conducted according to preliminary studies [ 22 , 23 ]. Briefly, after the human umbilical cord mesenchymal cells were cultured in DMEM-low glucose for 72 h, the detached and suspended cells in the fresh medium were centrifuged at 400 g and 15000 g for 10 min, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The method described in a previous study [ 22 ] was applied to the hydroxyproline content assay of the lung samples from each rat. Lung tissues were powdered using liquid N 2 and 30 mg of each tissue was transferred into a micro tube.…”

Section: Methodsmentioning

confidence: 99%

The Restoring Effect of Human Umbilical Cord-Derived Mesenchymal Cell-Conditioned Medium (hMSC-CM) against Carbon Tetrachloride-Induced Pulmonary Fibrosis in Male Wistar Rats

Khajvand-Abedini

Bahmani

Ziamajidi

et al. 2022

International Journal of Inflammation

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Objective. Pulmonary toxicity induced by CCl4, a model of idiopathic pulmonary fibrosis (IPF), leads to tissue remodeling and inflammation. Human umbilical cord mesenchymal cell-conditioned medium (hMSC-CM) is a potent anti-inflammatory, antioxidative, and antifibrotic agent. Methods. Forty male Wistar rats were assigned to the control (C), olive oil control (C.O) (hMSC-CM), control (C.Ms), fibrosis (fb), and fibrosis with hMSC-CM (f.Ms) treatment groups. The groups C, C.O, and C.Ms received PBS (200 µl), olive oil (1 ml/kg), and hMSC-CM (100 μg protein/kg), respectively. The fibrosis group was administered with only CCl4 (1 ml/kg). The last group, f.Ms was treated with CCl4 (1 ml/kg) and 100 μg protein/kg IV hMSC-CM. While the treatment with olive oil and CCl4 was performed for 2 days/week from the first week for 12 weeks, the treatment with PBS and hMSC-CM was carried out 2 days/week from week 4th to week 12th. The effect of the UC-MSC culture medium treatment on the lung was evaluated by assessing lysyl oxidase (LOX), tumor necrosis factor-alpha (TNF-α), and transforming growth factor-β1 (TGF-β1) genes, and proteins expression by real-time RCR and western blotting, respectively. Results. Lysyl oxidase (LOX), tumor necrosis factor-alpha (TNF-α), transforming growth factor-b1 (TGF-β1), malondialdehyde (MDA), and oxidative stress levels were markedly higher in the fibrosis group than in the control groups ( p ≤ 0.001 ). Additionally, glutathione (GSH) in the fibrosis group was markedly lower than those in the control groups ( p ≤ 0.001 ). Fibrosis in the UC-MSC treatment group had milder histopathological injuries than in the fibrosis group. Conclusion. hMSC-MSC as a strong anti-inflammatory, antioxidative, and antifibrotic decreases the level of oxidative stress, proinflammatory cytokines, and MDA causing a restoring effect against CCl4-induced pulmonary fibrosis.

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“…• Subgroup 1c: included nine rats. This group were injected with 200 μl MSCs-CM through the tail vein for each rat [20] twice per week for three weeks [21] .…”

Section: Experimental Protocolmentioning

confidence: 99%

The Therapeutic Potential of Stem Cells-Conditioned Media on Histologic Changes of Immune Checkpoints Inhibitor Pembrolizumab on Rats Thyroid Gland

Mohamed,

Abd Allah,

El-Mahroky

2022

Journal of Medical Histology

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Background: Immunological checkpoints inhibitor (ICI) Pembrolizumab (PEMB) is a monoclonal antibody that induces a potent antitumor immune response. Hypothyroidism and adrenal insufficiency have been associated with its addition to the therapy protocol. Conditioned media (CM) is a culture media for stem cells comprising soluble proteins, lipids, nucleic acids, and extracellular vesicles (EV). Aim of the Work: This research aimed to determine whether mesenchymal stem cells conditioned media (MSCs-CM) could ameliorate thyroid gland histological disruptions caused by pembrolizumab. Materials and Methods: 45 adult male rats were incorporated in this work and classified into: control group (I), Pembrolizumab treated group (II): Pembrolizumab was administered intraperitoneally (i.p.) at a dose of 3 mg/kg three times a week for four weeks, and conditioned media treated group (III): was treated as group II and then received 200μl of MSCs-CM via tail vein twice a week for three weeks. We measured thyroid hormones and oxidative markers. The thyroid gland was examined by Light and electron microscopy. Statistics were used to analyze all the data. Results: PEMB group shows desquamation of follicular cells into the lumen, cytoplasmic vacuolations and wide interstitial spaces among follicles. Some follicles appear involuted, others appear dilated and some follicles lack colloid. MSCs-CM group shows improvement in the thyroid sections' structural appearance. The majority of the follicles are colloid-filled. Cuboidal cells having large, rounded central nuclei lined the majority of thyroid follicles. Some follicles still have vacuolated cytoplasm. Conclusions: MSCs-CM is found to play a therapeutic role to improve PEMB thyroid ultrastructure histological alterations.

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Human umbilical cord-derived mesenchymal stem cells conditioned medium ameliorates CCl₄-induced liver fibrosis through regulation of expression and activity of liver lysyl oxidase

Cited by 4 publications

References 41 publications

The Efficiency of Stem Cells (SCs) Differentiation into Functional Hepatocytes for Treating Liver Disorders: A Systematic Review

The Efficiency of Stem Cells (SCs) Differentiation into Functional Hepatocytes for Treating Liver Disorders: A Systematic Review

The Restoring Effect of Human Umbilical Cord-Derived Mesenchymal Cell-Conditioned Medium (hMSC-CM) against Carbon Tetrachloride-Induced Pulmonary Fibrosis in Male Wistar Rats

The Therapeutic Potential of Stem Cells-Conditioned Media on Histologic Changes of Immune Checkpoints Inhibitor Pembrolizumab on Rats Thyroid Gland

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Human umbilical cord-derived mesenchymal stem cells conditioned medium ameliorates CCl4-induced liver fibrosis through regulation of expression and activity of liver lysyl oxidase

Cited by 4 publications

References 41 publications

The Efficiency of Stem Cells (SCs) Differentiation into Functional Hepatocytes for Treating Liver Disorders: A Systematic Review

The Efficiency of Stem Cells (SCs) Differentiation into Functional Hepatocytes for Treating Liver Disorders: A Systematic Review

The Restoring Effect of Human Umbilical Cord-Derived Mesenchymal Cell-Conditioned Medium (hMSC-CM) against Carbon Tetrachloride-Induced Pulmonary Fibrosis in Male Wistar Rats

The Therapeutic Potential of Stem Cells-Conditioned Media on Histologic Changes of Immune Checkpoints Inhibitor Pembrolizumab on Rats Thyroid Gland

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Human umbilical cord-derived mesenchymal stem cells conditioned medium ameliorates CCl₄-induced liver fibrosis through regulation of expression and activity of liver lysyl oxidase