Human-tumor-derived cell lines contain common and different transforming genes

Perucho, Manuel; Goldfarb, Mitchell; Shimizu, Kenji; Lama, Concepcion; Fogh, Jørgen; Wigler, Michael

doi:10.1016/0092-8674(81)90388-3

Cited by 419 publications

(206 citation statements)

References 19 publications

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“…This calculated value is similar to that reported with cloned Ha-ras-1 gene by other workers (Goldfarb et al, 1982;Shih & Weinberg, 1982 The effect of medium composition on the behaviour of the focus induction assay is striking; each medium tested in this study is used for the culture of fibroblast lines in various laboratories (Balmain & Pragnell, 1983;Brooks et al, 1983;Fukui et al, 1985;Perucho et al, 1981;Pulciano et al, 1982a;Shimuzu et al, 1985). The effects noted in our work may help to explain the differences in transforming potential in the focus assay performed by various investigators using different media.…”

supporting

confidence: 74%

“…The dishes were refed with fresh medium either every 3 days as in most previous studies using the focus induction assay (Albino et al, 1984;Copeland et al, 1979;Fukui et al, 1985;Gambke et al, 1984;Perucho et al, 1981;Pulciano et al, 1982a, b;Shimuzu et al, 1985;Takahashi et al, 1985), or every day. A pilot study showed that there was no significant difference between regimes of 3 days interval and 2 days interval.…”

mentioning

confidence: 99%

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Optimisation of conditions for detection of activated oncogenes by transfection of NIH 3T3 cells

Lemoine¹,

Wynford-Thomas²,

Wynford-Thomas³

1987

Br J Cancer

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supporting

confidence: 74%

mentioning

confidence: 99%

Optimisation of conditions for detection of activated oncogenes by transfection of NIH 3T3 cells

Lemoine¹,

Wynford-Thomas²,

Wynford-Thomas³

1987

Br J Cancer

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“…Although a gross chromosomal abnormality was not associated with all the parameters that showed heterogeneity, this does not conflict with the hypothesis that there is a genetic basis for heterogeneity in tumours (Nowell, 1976) as the changes responsible are very likely to be at the molecular level and therefore not detectable by the chromosome banding techniques used in this study. Recent evidence for a molecular change in the DNA being responsible for the onset of neoplasia has been proposed using transfection techniques (Krontiris and Cooper, 1981;Parada et al, 1982;Perucho et al, 1981). Molecular DNA clones-oncogenes-which have been isolated from human bladder and other tumour lines have been shown to transform mouse fibroblast lines (Shih et al, 1981).…”

Section: Discussionmentioning

confidence: 99%

Cellular heterogeneity in a tissue culture cell line derived from a human bladder carcinoma

Hastings¹,

Franks²

1983

Br J Cancer

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Summary To study heterogeneity in a cell line derived from a human bladder carcinoma (EJ), 7 clones were isolated at low passage and examined for differences in culture behaviour, ability to grow in agar and tumorigenicity in nude mice. The parent EJ line had several distinct chromosome populations (both diploid and tetraploid), grew in agar and produced tumours in nude mice. Three of the clones had pseudodiploid modes and 4 had either hypo-or hypertetraploid modes. The 7 clones had 5 marker chromosomes in common but the combination of other marker chromosomes made each clone unique. No Differences were also found in the packing density of the cloned cells and in the cell size. All 7 clones grew in agar but heterogeneity was seen between the clones as shown by widely varying colony-forming efficiencies (0.5-13%). One clone had a high colony-forming ability in agar but failed to produce tumours in nude mice. The other clones were tumorigenic regardless of colony-forming efficiency in agar. Specific chromosome abnormalities were found to be associated with growth in agar and tumorigenicity but not with the growth pattern or the rate of movement of the cloned cells in culture.

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“…DNA extraction and PCR-RFLP High molecular weight genomic DNA was extracted from the patients' peripheral blood lymphocytes after proteinase K digestion by the standard phenol-chloroform method (Perucho et al, 1981). The modified PCR-RFLP method for HLA-DRB1 genotyping described by Ota et al (1992) and that for HLA-DQB1 described by Nomura et al (1991) were used.…”

Section: Methodsmentioning

confidence: 99%

High-resolution HLA-DRB1 and DQB1 genotyping in Japanese patients with testicular germ cell carcinoma

Özdemir

Kakehi²,

Mishina³

et al. 1997

Br J Cancer

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Summary We report for the first time the frequency distributions of HLA-DRB1 and -DQB1 genes in 55 patients with testicular germ cell carcinoma (TGC) using the modified PCR-RFLP method and compare the results with those for 1216 healthy Japanese control subjects. The modified PCR-RFLP method produced accurate, reproducible cleavage patterns that are easily discriminated. HLA-DRB1*0410 was the susceptibility allele (RR = 3.26, P = 0.006) and DQB1 *0602 appears to be a candidate protective allele (RR = 0.26, P = 0.02) for TGC in the Japanese. None of the HLA-DRB1 and -DQB1 alleles showed a specific tendency for histological type or clinical stage of the tumours. Previous studies based on serotyping methods failed to show these allelic associations. High-resolution genotyping is essential because the peptide-binding domain of MHC class 11 molecules is determined more precisely by their genotypes than by their serotypes. In addition, inherent technical difficulties and typing errors of up to 25% make serotyping inefficient. Our results suggest that high-resolution genotyping is a useful genetic marker to determine risk for TGC.Keywords: testicular germ cell carcinoma; MHC class 11 genotyping; modified PCR-RFLP; allele frequency Testicular germ cell carcinoma (TGC), although relatively rare in the male population in general, is the most common malignancy in men between the ages of 15 and 35 years. Strong evidence for the involvement of genetic factors has been reported in TGC development. The incidence of TGC in first degree relatives is 2.2%, and in unrelated individuals 0.4%. For an individual who has a father or brother with TGC, the relative risk of developing TGC is increased sixfold compared with men in the general population. The concordance of tumour histology is high in twins and low in father-son pairs (Tolerud et al, 1985). Moreover, the incidence of TGC is relatively high among white American men in comparison with black American or Oriental men living in the same geographical areas

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Human-tumor-derived cell lines contain common and different transforming genes

Cited by 419 publications

References 19 publications

Optimisation of conditions for detection of activated oncogenes by transfection of NIH 3T3 cells

Optimisation of conditions for detection of activated oncogenes by transfection of NIH 3T3 cells

Cellular heterogeneity in a tissue culture cell line derived from a human bladder carcinoma

High-resolution HLA-DRB1 and DQB1 genotyping in Japanese patients with testicular germ cell carcinoma

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