Human transient receptor potential (TRP) channel expression profiling in carcinogenesis

Bernardini, Michela; Fiorio, Alessandra; Prevarskaya, Natalia; Gkika, Dimitra

doi:10.1387/ijdb.150232dg

Cited by 26 publications

(24 citation statements)

References 71 publications

(74 reference statements)

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“…Partial TRP expression profiles have appeared for a variety of murine tissues (35), including adipose (18). A comprehensive profile of TRP channel expression in human adipose tissues and associated progenitor cells is currently lacking (36). We analyzed the expression pattern of all human TRP channels during in vitro adipogenesis and found strong constitutive expression of TRPs P2, M7, V1, V2, V4, C1, C4, and ML1 in both MSCs and SVF cells, regardless of differentiation status, reflecting their generalized role in cell growth (proliferation and metabolism) and survival (37).…”

Section: Discussionmentioning

confidence: 99%

TRP channels in brown and white adipogenesis from human progenitors: new therapeutic targets and the caveats associated with the common antibiotic, streptomycin

et al. 2017

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Transient receptor potential (TRP) channels are polymodal cell sensors responding to diverse stimuli and widely implicated in the developmental programs of numerous tissues. The evidence for an involvement of TRP family members in adipogenesis, however, is scant. We present the first comprehensive expression profile of all known 27 human TRP genes in mesenchymal progenitors cells during white or brown adipogenesis. Using positive trilineage differentiation as an exclusion criterion, TRP polycystic (P)3, and TPR melastatin (M)8 were found to be uniquely adipospecific. Knockdown of TRPP3 repressed the expression of the brown fat signature genes uncoupling protein (UCP)-1 and peroxisome proliferator-activated receptor γ coactivator (PGC)-1α as well as attenuated forskolin-stimulated uncoupled respiration. However, indices of generalized adipogenesis, such as lipid droplet morphology and fatty acid binding protein (FAPB)-4 expression, were not affected, indicating a principal mitochondrial role of TRPP3. Conversely, activating TRPM8 with menthol up-regulated UCP-1 expression and augmented uncoupled respiration predominantly in white adipocytes (browning), whereas streptomycin antagonized TRPM8-mediated calcium entry, downregulated UCP-1 expression, and mitigated uncoupled respiration; menthol was less capable of augmenting uncoupled respiration (thermogenesis) in brown adipocytes. TRPP3 and TRPM8 hence appear to be involved in the priming of mitochondria to perform uncoupled respiration downstream of adenylate cyclase. Our results also underscore the developmental caveats of using antibiotics in adipogenic studies.-Goralczyk, A., van Vijven, M., Koch, M., Badowski, C., Yassin, M. S., Toh, S.-A., Shabbir, A., Franco-Obregón, A., Raghunath, M. TRP channels in brown and white adipogenesis from human progenitors: new therapeutic targets and the caveats associated with the common antibiotic, streptomycin.

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Section: Discussionmentioning

confidence: 99%

TRP channels in brown and white adipogenesis from human progenitors: new therapeutic targets and the caveats associated with the common antibiotic, streptomycin

et al. 2017

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“…In particular, it has been shown that changes in the expression of TRP channels are correlated with the progression of different types of cancer. To date, most changes involving TRP proteins do not involve mutations in the TRP gene but rather dysregulation of the wild-type TRP protein expression levels, depending on the stage of cancer ( Gkika and Prevarskaya, 2011 ; Lehen’kyi and Prevarskaya, 2011 ; Bernardini et al, 2015 ; Shapovalov et al, 2016 ). Moreover, several recent studies have highlighted a direct correlation between cancer patient survival and TRP channel expression.…”

Section: Introductionmentioning

confidence: 99%

TRP Channels and Small GTPases Interplay in the Main Hallmarks of Metastatic Cancer

2020

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Transient Receptor Potential (TRP) cations channels, as key regulators of intracellular calcium homeostasis, play a central role in the essential hallmarks of cancer. Among the multiple pathways in which TRPs may be involved, here we focus our attention on the ones involving small guanosine triphosphatases (GTPases), summarizing the main processes associated with the metastatic cascade, such as migration, invasion and tumor vascularization. In the last decade, several studies have highlighted a bidirectional interplay between TRPs and small GTPases in cancer progression: TRP channels may affect small GTPases activity via both Ca 2+ -dependent or Ca 2+ -independent pathways, and, conversely, some small GTPases may affect TRP channels activity through the regulation of their intracellular trafficking to the plasma membrane or acting directly on channel gating. In particular, we will describe the interplay between TRPC1, TRPC5, TRPC6, TRPM4, TRPM7 or TRPV4, and Rho-like GTPases in regulating cell migration, the cooperation of TRPM2 and TRPV2 with Rho GTPases in increasing cell invasiveness and finally, the crosstalk between TRPC1, TRPC6, TRPM8, TRPV4 and both Rho- and Ras-like GTPases in inducing aberrant tumor vascularization.

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“…It follows that cells may experience different status, such as growth, differentiation, or death. Particularly, the abnormal conditions of ion channel trigged by mechanical force, that is, the abnormal switch on-off and excessive open or close, can induce cell apoptosis and death (see reviews [94][95][96]). It provides us with a new thought for cell regulation or diseases therapy.…”

Section: Under Constant or Low Frequency Magnetic Fieldmentioning

confidence: 99%

Mechanisms of Cellular Effects Directly Induced by Magnetic Nanoparticles under Magnetic Fields

Chen

Wang

et al. 2017

Journal of Nanomaterials

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The interaction of magnetic nanoparticles (MNPs) with various magnetic fields could directly induce cellular effects. Many scattered investigations have got involved in these cellular effects, analyzed their relative mechanisms, and extended their biomedical uses in magnetic hyperthermia and cell regulation. This review reports these cellular effects and their important applications in biomedical area. More importantly, we highlight the underlying mechanisms behind these direct cellular effects in the review from the thermal energy and mechanical force. Recently, some physical analyses showed that the mechanisms of heat and mechanical force in cellular effects are controversial. Although the physical principle plays an important role in these cellular effects, some chemical reactions such as free radical reaction also existed in the interaction of MNPs with magnetic fields, which provides the possible explanation for the current controversy. It is anticipated that the review here could provide readers with a deeper understanding of mechanisms of how MNPs contribute to the direct cellular effects and thus their biomedical applications under various magnetic fields.

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Human transient receptor potential (TRP) channel expression profiling in carcinogenesis

Cited by 26 publications

References 71 publications

TRP channels in brown and white adipogenesis from human progenitors: new therapeutic targets and the caveats associated with the common antibiotic, streptomycin

TRP channels in brown and white adipogenesis from human progenitors: new therapeutic targets and the caveats associated with the common antibiotic, streptomycin

TRP Channels and Small GTPases Interplay in the Main Hallmarks of Metastatic Cancer

Mechanisms of Cellular Effects Directly Induced by Magnetic Nanoparticles under Magnetic Fields

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