Human Th1 Cells That Express CD300a Are Polyfunctional and After Stimulation Up-Regulate the T-Box Transcription Factor Eomesodermin

Narayanan, Sriram; Silva, Rodolfo; Peruzzi, Giovanna; Alvarez, Yelina; Simhadri, Venkateswara R.; DeBell, Karen E.; Coligan, John E.; Borrego, Francisco

doi:10.1371/journal.pone.0010636

Cited by 37 publications

(58 citation statements)

References 56 publications

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“…2 (10,15). In contrast, in human CD8 + T cells, CD300a expression is coupled to a more cytotoxic phenotype, suggesting that CD300a is mostly expressed on effector/ memory cells, whereas naive CD8 + T cells express low levels of the receptor (17).…”

Section: Regulation Of Expressionmentioning

confidence: 99%

“…With regard to the lymphoid lineage, CD300a is expressed on the surface of all human NK cells (9,14) and in subsets of T and B cells (8,10,(15)(16)(17). Human naive CD4 + T cells express low levels of CD300a, whereas effector/memory cells can be subdivided into CD300a + and CD300a 2 subsets, and regulatory T cells are CD300a 2 .…”

Section: Regulation Of Expressionmentioning

confidence: 99%

“…Human naive CD4 + T cells express low levels of CD300a, whereas effector/memory cells can be subdivided into CD300a + and CD300a 2 subsets, and regulatory T cells are CD300a 2 . Memory CD300a 2 cells tend to proliferate slightly less than do memory CD300a + cells (8,15), and the expression of CD300a is associated with Th1 cells that are more polyfunctional and, after stimulation, upregulate the T-box transcription factor eomesodermin, whereas the IL-17 single-producing CD4 + T cells are mostly CD300a…”

Section: Regulation Of Expressionmentioning

confidence: 99%

“…For example, in CD4 + T cells, stimulation with anti-CD3 plus anti-CD28 mAbs and Th1-differentiation conditions upregulate cell surface expression of CD300a, whereas TGF-b exhibits a negative regulatory effect (8,15). These in vitro-obtained results are in agreement with the fact that TGF-b1 is required for the development of regulatory T cells, which are CD300a 2 , and Th17 cells, which tend to be enriched in the CD300a 2 subset (10, 15).…”

Section: Regulation Of Expressionmentioning

confidence: 99%

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The Biology and Disease Relevance of CD300a, an Inhibitory Receptor for Phosphatidylserine and Phosphatidylethanolamine

Zenarruzabeitia

Vitallé

Eguizábal³

et al. 2015

The Journal of Immunology

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The CD300a inhibitory receptor belongs to the CD300 family of cell surface molecules that regulate a diverse array of immune cell processes. The inhibitory signal of CD300a depends on the phosphorylation of tyrosine residues embedded in ITIMs of the cytoplasmic tail. CD300a is broadly expressed on myeloid and lymphoid cells, and its expression is differentially regulated depending on the cell type. The finding that CD300a recognizes phosphatidylserine and phosphatidylethanolamine, two aminophospholipids exposed on the outer leaflet of dead and activated cells, has shed new light on its role in the modulation of immune functions and in its participation in the host response to several diseases states, such as infectious diseases, cancer, allergy, and chronic inflammatory diseases. This review summarizes the literature on CD300a expression, regulation, signaling pathways, and ligand interaction, as well as its role in fine tuning immune cell functions and its clinical relevance.

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Section: Regulation Of Expressionmentioning

confidence: 99%

Section: Regulation Of Expressionmentioning

confidence: 99%

Section: Regulation Of Expressionmentioning

confidence: 99%

Section: Regulation Of Expressionmentioning

confidence: 99%

See 2 more Smart Citations

The Biology and Disease Relevance of CD300a, an Inhibitory Receptor for Phosphatidylserine and Phosphatidylethanolamine

Zenarruzabeitia

Vitallé

Eguizábal³

et al. 2015

The Journal of Immunology

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“…This inhibitory receptor is constitutively expressed in a variety of immune cells including NK cells (8)(9)(10), T helper cells (11,12), monocytes (13), neutrophils (14), eosinophils (15), dendritic cells (10)(11)(12)(13)(14)(15)(16), and mast cells (17).…”

mentioning

confidence: 99%

CD300a is expressed on human basophils and seems to inhibit IgE/FcεRI‐dependent anaphylactic degranulation

Sabato

Verweij

Bridts

et al. 2011

Cytometry Part B Clinical

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Background. The final response that leads to basophil degranulation results from a cross-talk between activatory and inhibitory signals. However, in the context of basophil biology, the inhibitory mechanisms that control these processes are still poorly understood.Aim. To investigate the expression and function of the inhibitory receptor CD300a in human basophils. Methods. Peripheral blood of 20 patients with birch pollen allergy and 10 healthy control individuals were assessed for CD300a expression before and after activation. To study the function of CD300a, basophils were pre-incubated with anti-human CD300a/c monoclonal antibodies and analyzed for the upregulation of the activation marker CD203c and appearance of the degranulation marker CD63 following IgE-dependent and IgE-independent triggering.Results. Basophils from allergic individuals constitutively expressed significantly less CD300a than non-allergics (P 5 0.001). However, after cross-linking with either anti-IgE or recombinant major birch pollen allergen (rBet v 1), basophils from allergic patients demonstrated a significant and rapid (3 min) up-regulation of CD300a expression in all activated basophils that persisted for over 2 h (P < 0.05). Moreover, CD300a expression was significantly higher in the CD203c bright1 CD63 bright1 subpopulation than in CD203c bright1 CD63 2 cells (P < 0.05). In both patients and controls, pre-incubation with antiCD300a/c significantly inhibited IgE-mediated CD63 expression (P < 0.05), though it did not affect CD203c. In contrast, IgE-independent basophil activation was not inhibited by CD300a/c engagement.Conclusion. CD300a is expressed on human peripheral blood basophils and rapidly up-regulated upon cross-linking of IgE/FceRI and suppresses anaphylactic degranulation. V C 2011 International Clinical Cytometry Society

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CD300 receptor family in viral infections

et al. 2018

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The CD300 molecules constitute an evolutionarily significant family of receptors that are expressed on myeloid and lymphoid cells, but also on other cell types, such as tuft cells. Many of the CD300 receptors have been shown to recognize lipids, e.g. phosphatidylserine and phosphatidylethanolamine. Over the past couple of years, accumulating evidence has shown that this family of receptors is involved in the pathogenesis of many diseases. Specifically, CD300 molecules participate in the mechanisms that viruses employ to develop immune evasion strategies and to infect host cells. The participation of CD300 molecules in viral infection includes both lipid dependent and independent mechanisms, as for example in infections with dengue virus (DENV) and murine norovirus (MNV), respectively. CD300 receptors are also involved in viral escape mechanisms, for instance inhibiting NK cell‐mediated cytotoxicity against infected cells. Moreover, it is becoming increasingly recognized that the expression of CD300 receptors is altered during viral diseases. Here, we review the involvement of human and murine CD300 molecules in viral binding and entry and in cellular responses to viruses, which highlights the potential of CD300 molecules in the search of new biomarkers for various stages of infection and therapeutic targets for the treatment of viral infections.

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Human Th1 Cells That Express CD300a Are Polyfunctional and After Stimulation Up-Regulate the T-Box Transcription Factor Eomesodermin

Cited by 37 publications

References 56 publications

The Biology and Disease Relevance of CD300a, an Inhibitory Receptor for Phosphatidylserine and Phosphatidylethanolamine

The Biology and Disease Relevance of CD300a, an Inhibitory Receptor for Phosphatidylserine and Phosphatidylethanolamine

CD300a is expressed on human basophils and seems to inhibit IgE/FcεRI‐dependent anaphylactic degranulation

CD300 receptor family in viral infections

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