Human T cells monitored by impedance spectrometry using field-effect transistor arrays: A novel tool for single-cell adhesion and migration studies

Law, Jessica Ka-Yan; Susloparova, Anna; Vu, Xuan Thang; Zhou, Xiao Qin; Hempel, Felix; Qu, Bin; Hoth, Markus; Ingebrandt, Sven

doi:10.1016/j.bios.2014.08.007

Cited by 21 publications

(14 citation statements)

References 28 publications

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“…The wafer was diced and encapsulated for cell culture as described in previous publications (Law et al 2015;Rahman et al 2007). The glass chips were wire bonded to carriers (LeitOn GmbH, Germany) and encapsulated with glass rings using silicone adhesive (Dow Corning, 96-083) to generate a cell-culture chamber.…”

Section: Fabrication Of Oect Devicesmentioning

confidence: 99%

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PEDOT:PSS organic electrochemical transistor arrays for extracellular electrophysiological sensing of cardiac cells

Hempel

Law

Nguyen

et al. 2017

Biosensors and Bioelectronics

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Section: Fabrication Of Oect Devicesmentioning

confidence: 99%

“…The process flow (Fig. S2) required much less fabrication steps compared to our silicon FETs (Susloparova et al, 2015). Most importantly no ion implantation is necessary and the polymer material itself is very cheap.…”

Section: Introductionmentioning

confidence: 99%

PEDOT:PSS organic electrochemical transistor arrays for extracellular electrophysiological sensing of cardiac cells

Hempel

Law

Nguyen

et al. 2017

Biosensors and Bioelectronics

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“…Despite the vast literature (reviewed in [37] up to 2010), single-cell IFC has not yet reached the same maturity and diffusion of ECIS. From the analysis of recent advances in the field of single-cell detection [44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59], the following trends can be highlighted. (1) Smarter and more robust event detection algorithms are emerging.…”

Section: Applicationmentioning

confidence: 99%

Advances in High-Resolution Microscale Impedance Sensors

Carminati

2017

Journal of Sensors

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Sensors based on impedance transduction have been well consolidated in the industry for decades. Today, the downscaling of the size of sensing elements to micrometric and submicrometric dimensions is enabled by the diffusion of lithographic processes and fostered by the convergence of complementary disciplines such as microelectronics, photonics, biology, electrochemistry, and material science, all focusing on energy and information manipulation at the micro- and nanoscale. Although such a miniaturization trend is pivotal in supporting the pervasiveness of sensors (in the context of mass deployment paradigms such as smart city, home and body monitoring networks, and Internet of Things), it also presents new challenges for the detection electronics, reaching the zeptoFarad domain. In this tutorial review, a selection of examples is illustrated with the purpose of distilling key indications and guidelines for the design of high-resolution impedance readout circuits and sensors. The applications span from biological cells to inertial and ultrasonic MEMS sensors, environmental monitoring, and integrated photonics.

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“…16 One attractive approach is the use of AlGaN/GaN high electron mobility transistors (HEMTs) functionalized with antibody or aptamer over the active gate channel. [17][18][19][20][21][22] The AlGaN/GaN HEMTs have demonstrated superior biosensing characteristics due to a high density two-dimensional electron gas (2DEG) channel located close the surface (around 25 nm) and very sensitive to changes in surface charges. [21][22][23][24][25][26][27] bio-sensing applications with the antibody immobilized directly over the active gate channel, the detection would not be very consistent for high ionic strength solutions such as human blood or serum.…”

mentioning

confidence: 99%

Zika virus detection using antibody-immobilized disposable cover glass and AlGaN/GaN high electron mobility transistors

Yang

Carey

Ren

et al. 2018

Applied Physics Letters

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Zika virus detection was demonstrated using antibody-functionalized cover glasses externally connected to the gate electrode of an AlGaN/GaN high electron mobility transistor (HEMT). A pulsed bias voltage of 0.5 V was applied to an electrode on the region of the cover glass region functionalized with antibody, and the resulting changes of drain current of the HEMT were employed to determine the presence of Zika virus antigen concentration ranging from 0.1 to 100 ng/ml. The dynamic and static drain current changes as a function of Zika virus concentration were modeled with a spring-like elastic relaxation model and the Langmuir extension model, respectively. Excellent fits to the data were found with relaxation time constants of antibody and antigen molecules in the range of 11 ls and 0.66-24.4 ls, respectively, for the concentration range investigated. The ratio of antibody bound with antigen to the total available antibody on the functionalized contact window was in the range of 0.013-0.84 for the Zika antigen concentration range of 0.1-100 ng/ml. Since the HEMT is not exposed to the bio-solution, it can be used repeatedly. The functionalized glass is the only disposable part in the detection system, showing the potential of this approach for hand-held, low cost sensor packages for point-of-care applications. Published by AIP Publishing.

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Human T cells monitored by impedance spectrometry using field-effect transistor arrays: A novel tool for single-cell adhesion and migration studies

Cited by 21 publications

References 28 publications

PEDOT:PSS organic electrochemical transistor arrays for extracellular electrophysiological sensing of cardiac cells

PEDOT:PSS organic electrochemical transistor arrays for extracellular electrophysiological sensing of cardiac cells

Advances in High-Resolution Microscale Impedance Sensors

Zika virus detection using antibody-immobilized disposable cover glass and AlGaN/GaN high electron mobility transistors

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