Human T cell receptor for IgM: specificity for the pentameric Fc fragment

Abstract

The specificity of the IgM receptor expressed by human T cells cultured in IgM-free media has been investigated. IgM receptors have been detected using a rosette system with ox erythrocytes coated with rabbit antibody (EA(IgM)), and the inhibitory capacity of different IgM fragments in the rosette system has been tested. It was found that F(c)5mu but not F(ab')2mu, nor monomeric IgM (8 S IgM) inhibited EA (IgM) rosette-forming cells. This indicates that the receptor present on the surface of T cells has affinity for a structure located in the Fc portion of the pentameric IgM.

The Differential Effect of In Vivo Hydrocortisone on the Kinetics of Subpopulations of Human Peripheral Blood Thymus-Derived Lymphocytes

“…At 24 h, there was a rebound increase in the numbers of T., Thtus, the data show that 4 In ain elegant series of papers, Moretta anid his colleagues have described the T.M sulbset of human T lymphocytes which comiiprise approximately 55-7.5% of humiicani T cells aild which bind the Fe portion of the pentamiieric formii of IgM (8,13). Other laboratories have now confirmiied these finidings in human T cells (14) and, as well, have demonstratedl T.M cells in m1o1Use T-cell sUspen1sion1s (15).…”

Recombinant chimeric OKT3 scFv IgM antibodies mediate immune suppression while reducing T cell activationin vitro

“…R. Monomeric subunits are less efficient in binding to the Fc ? R [41,42] which is mainly present on a small fraction of B lymphocytes and NK cells [43]. IgM-induced cross-linking of Fc ?…”

The in vitro development of immunoglobulin producing cells from the human bone marrow null lymphocyte.

“…The E and Eox A, double rosette forming cells were considered as Fc(,u) receptor positive T cells (Tµ) (Moretta et al 1975;Ferrarini et al 1976). K cells One tenth ml of lymphocyte suspension in 0.3% agar (105/mm3) was put on the 2% EA suspension in 0.5% agar plate in a plastic petri dish (35 X 10 mm).…”