The Differential Effect of In Vivo Hydrocortisone on the Kinetics of Subpopulations of Human Peripheral Blood Thymus-Derived Lymphocytes

Haynes, Barton F.; Fauci, Anthony S.

doi:10.1172/jci108982

Cited by 188 publications

(53 citation statements)

References 16 publications

(22 reference statements)

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“…Lymphocyte subsets and their level of variability also differ in subjects with diseases such as SLE (6,(15)(16)(17). Therapy with corticosteroids and other immunomodulatory agents can have profound effects on lymphocyte subset distributions as well (2,(18)(19)(20)(21), further complicating longitudinal data interpretation in the setting of disease.…”

Section: Discussionmentioning

confidence: 99%

Circulating lymphocyte subsets in normal adults are variable and can be clustered into subgroups

Sekiguchi

Smith

Sutter

et al. 2011

Cytometry Part B Clinical

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Background: Flow cytometry is used to monitor lymphocyte subsets in both the clinical and research settings. An understanding of the degree of inter-and intrasubject variability of these populations is critical for data interpretation.Methods: Peripheral blood lymphocytes of 18 healthy adults were analyzed on two separate occasions using a multicolor flow cytometric panel with B, T, and NK cell markers. Variability was calculated using the coefficient of variation and compared between and within individuals using agglomerative clustering.Results: Each subject appears to have B and T cell subset profiles that are stable over the two time points, but differ from the profiles of other subjects. Thus, the range of measurements for a particular B or T cell subset is larger between subjects and narrower for an individual. In addition, the level of variability correlates inversely with the size of the lymphocyte subset. When lymphocyte profiles are analyzed by agglomerative clustering, replicate samples from the same individual tend to cluster. When single samples from different individuals are analyzed, individuals appear to cluster into different subgroups.Conclusions: Variability of lymphocyte subsets is usually greater between individuals than within a single individual and each person appears to have a characteristic profile of lymphocyte subsets. These results underscore the importance of obtaining a baseline value for each subject when investigating the impact of a treatment on lymphocyte subsets over time. These results also highlight the potential utility of cluster analysis as a tool for immune subset profiling and biomarker discovery.

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Section: Discussionmentioning

confidence: 99%

Circulating lymphocyte subsets in normal adults are variable and can be clustered into subgroups

Sekiguchi

Smith

Sutter

et al. 2011

Cytometry Part B Clinical

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“…WALKER et al [42] reported that T-cell activation and the cytokine pattern in BAL differed between atopic asthma and nonatopic asthma. A possible reason is that steroids may decrease the number of lymphocytes -mainly T-cells [43], especially CD4-positive cells [44,45], in peripheral blood. ROBINSON et al [46] reported that the number of cells expressing messenger ribonucleic acid (mRNA) for IL-4 and IL-5 in BAL from asthmatic patients decreased significantly after steroid therapy.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effects of inhaled beclomethasone dipropionate in nonatopic asthmatics

Hoshino

Nakamura²

1996

Eur Respir J

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There was a significant improvement in the asthma symptom score, PEF%, FEV1%, and Dmin after BDP therapy and the number of EG2-, AA1-, CD3-, CD4-, and CD25-positive cells decreased significantly.We conclude that inhaled beclomethasone dipropionate inhibited inflammatory cell infiltration of airway tissue and that associated with this there was an improvement of symptoms in this open study of inhaled beclomethasone dipropionate in a group of nonatopic asthmatic subjects.

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“…Using Fc receptors for JgM and IgG to measure T helper and T suppressor cells, respectively, Haynes and Fauci (26) reported that corticosteroids markedly decreased T helper cells but had only modest effects on the number of T suppressor cells. These effects were transient, indicating a redistribution of subsets.…”

Section: Discussionmentioning

confidence: 99%

T Lymphocyte Subsets in Systemic Lupus Erythematosus

Bakke

Kirkland

Kitridou

et al. 1983

Arthritis & Rheumatism

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The contribution of immune regulation to the etiology of systemic lupus erythematosus (SLE) is poorly understood. Using the monoclonal antibodies OKT4 and OKTS, we quantitated, by flow cytometry, T inducer/ helper and T cytotoxicfsuppressor cells in patients with SLE. Serologically active patients, who had clinical manifestations such as arthritis or rash and were not receiving prednisone, were characteristically lymphopenic due to a marked reduction in OKT4+ cells. Prednisone therapy produced the same phenomenon. Untreated patients, who were serologically inactive, demonstrated no abnormalities. These studies have thus revealed two presumably independent factors that can produce similar immunoregulatory aberrations.Systemic lupus erythematosus (SLE) is an autoimmune disorder characterized by excessive immunoglobulin and autoantibody production. Although B cell function is increased, T cell functions which include delayed hypersensitivity (1-3, mitogenic reactivity (6,7), generation of antigen-specific reactivities (8), and development of nonspecific suppressor (14).We studied 28 patients with SLE who were divided into groups based on corticosteroid therapy and serologic evidence of disease activity. We will describe clinically significant abnormalities in the absolute number and relative proportions of immunoregulatory T cell subsets based upon this classification. PATIENTS AND METHODSPatients. This study included 28 patients (24 females, 4 males) with SLE according to the American Rheumatism Association ( M A ) preliminary criteria (15). Two to 3 patients were randomly selected in the lupus clinic visits each month from April 1981 to April 1982. These patients were then separated into 2 groups based on existing therapy, one receiving more than 10 mg prednisone per day, the other receiving 10 mg or less of prednisone per day. A special attempt was made to isolate newly diagnosed lupus patients being treated with 10 mg or less prednisone per day. During this 1-year period, 11 newly diagnosed patients were seen, but only 6 had 4 or more ARA preliminary criteria; of these 6, only 5 were treated with less than 10 mg prednisone per day.Control groups consisted of 16 women and 5 men with scleroderma and 16 patients with lepromatous leprosy. They will be described in detail elsewhere. Normal individ-

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The Differential Effect of In Vivo Hydrocortisone on the Kinetics of Subpopulations of Human Peripheral Blood Thymus-Derived Lymphocytes

Cited by 188 publications

References 16 publications

Circulating lymphocyte subsets in normal adults are variable and can be clustered into subgroups

Circulating lymphocyte subsets in normal adults are variable and can be clustered into subgroups

Anti-inflammatory effects of inhaled beclomethasone dipropionate in nonatopic asthmatics

T Lymphocyte Subsets in Systemic Lupus Erythematosus

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