Human Sperm Velocity and Postinsemination Cervical Mucus Test in the Evaluation of the Infertile Couple

Abstract

Homologous artificial insemination (AIH) followed by postinsemination cervical mucus test (PIT) was performed in 100 couples in a prospective study to evaluate suspected cervical or male factor infertility. A portion of the ejaculate was submitted for standard semen analysis as well as turbidimetric analysis of sperm velocity. In a review of 333 cases evaluated for infertility, the average sperm velocity in a normal semen analysis was 96.5 mu/sec, for those with normal postcoital test (PCT) 96.6 mu/sec, and in those that established a pregnancy 91.6 mu/sec. These are significantly higher than the values obtained for abnormal semen analysis and abnormal PCT (64.6 and 63.6 mu/sec, respectively; p less than 0.001). Patients with normal PIT (WHO criteria for normal PCT consisting of greater than or equal to 7 motile sperm/hpf) had sperm velocity of 87 mu/sec compared to velocities of 46 mu/sec for abnormal PIT (p less than 0.001). Four of the five patients with abnormal PIT (in spite of normal semen analysis and normal cervical mucus) had sperm velocities less than 75 mu/sec. Likewise, all 8 patients who had normal PIT in spite of abnormal semen analysis had sperm velocities greater than 75 mu/sec, even though the sperm motility was below normal in 5 of them. Sperm velocity is a more sensitive indicator of sperm function when compared to standard semen analysis results.

Usefulness of Sperm Quality Analyzer-v (Sqa-V) for the Assessment of Sperm Quality in Infertile Men

“…Same as SQA in former version [2,5,[9][10][11], since SQA-V recognizes only motile particles, problems with debris, which may have 440 T. Akashi et al influenced the difference of the sperm concentration between SQA-V and CASA, have been excluded. And poor conditions, including poor motion or low sperm concentration [2,19] also may influence the difference of the estimates between SQA-V and CASA. In the future, the development of the SQA-V with higher sensitivity to lower sperm quality is desirable.…”

Relationship between human sperm motility characteristics and sperm penetration into human cervical mucus in vitro

“…Furthermore, aspects of sperm movement such as the velocity of progression and the actual pattern of movement have been shown to be closely correlated with sperm penetration into bovine and human cervical mucus (Beauchamp, Galle & Blasco, 1984;Aitken et ai, 1985;Feneux, Serres & Jouannet, 1985).…”

The Expression of Cysteine-Rich Secretory Protein 2 (CRISP2) and Its Specific Regulator miR-27b in the Spermatozoa of Patients with Asthenozoospermia1

“…Low sperm motility is considered to be a more likely reason for male infertility [87], and sperm motility pattern is closely related with a natural pregnancy rate [88,89]. Low pregnancy rate has been reported to be due to low CRISP2 expression level in the ejaculated spermatozoa in cattle [33].…”