Relationship between human sperm motility characteristics and sperm penetration into human cervical mucus in vitro

Mortimer, David; Pandya, Ila J.; Sawers, R. S.

doi:10.1530/jrf.0.0780093

Cited by 136 publications

(73 citation statements)

References 22 publications

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“…Sperm morphology assessment is one example of classification as normal or abnormal based on set criteria for characteristics measured on continuous scales (e.g., head size and shape, acrosome size, proportion of the head occupied by vacuoles, midpiece size and contour, tail length, and size of the cytoplasmic residue). It is therefore reasonable to use data obtained by objective measurements to establish a cutoff point between the categories of slow and rapid progressive spermatozoa; 25 µm s -1 [14] is based on results derived from analysis of sperm kinematics [7,11]. It is less important whether the limit is 23 or 25 µm s -1 than that the presence of rapidly forward moving spermatozoa is assessed separately from spermatozoa with less progressive motility.…”

Section: Discussionmentioning

confidence: 99%

“…With in vitro techniques, fertilization and zygote/embryo cleavage rates are obvious measures. With in vivo fertilization, sperm-cervical mucus penetration tests and rapid progressive sperm motility have long been known to be useful indicators [6][7][8][9]. Irrespective of whether one adopts an in vitro or in vivo perspective, progressive sperm motility is thus required for success (with the exception of treatment by intracytoplasmic sperm injection [ICSI]).…”

Section: Introductionmentioning

confidence: 99%

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The usefulness and significance of assessing rapidly progressive spermatozoa

Björndahl¹

2010

Asian J Androl

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It is possible and clinically relevant to distinguish between slow and rapid progressive spermatozoa in basic semen analysis. This is discussed in light of the different purposes of semen analysis for the subfertile couple and the male patient. The two groups of progressive spermatozoa should be distinguished to help ensure that pertinent information available in the semen sample is not neglected.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The usefulness and significance of assessing rapidly progressive spermatozoa

Björndahl¹

2010

Asian J Androl

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“…It was shown in that study that the presence of SP in the thawing medium reduced the proportion of spermatozoa with B pattern by CTC (indicating increased plasma membrane stabilization), and also increased the proportion of spermatozoa able to penetrate cervical mucus in vitro. Cervical mucus penetration has been related to sperm motility, and specifically to spermatozoa with good linear progression and moderate amplitudes of lateral head displacement (Aitken et al 1985, Feneux et al 1985, Mortimer et al 1986). Therefore, it seems logical that observed differences in mucus-penetrating ability would be reflected in differences in sperm kinematics, as well as in differences in head plasma membrane stability.…”

Section: Discussionmentioning

confidence: 99%

“…Only seminal spermatozoa with good, progressive motility and a high amplitude of lateral head displacement are able to penetrate oestrous cervical mucus (Aitken et al 1985, Feneux et al 1985, Mortimer et al 1986. It is known that spermatozoa incubated under capacitating conditions, in the absence of SP, exhibit different movement patterns (kinematics) from seminal spermatozoa (which are the ones that penetrate cervical mucus in vivo) (reviewed by Mortimer, 1997).…”

Section: Introductionmentioning

confidence: 99%

Effect of medium on the kinematics of frozen–thawed ram spermatozoa

Mortimer¹,

Maxwell²

2004

Reproduction

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Cervically inseminated cryopreserved ram spermatozoa have reduced fertility due to poor mucus-penetrating ability. This effect is ameliorated by the addition of 20% (v/v) seminal plasma (SP) to the phosphate-buffered saline (PBS) thawing medium. The aims of this study were to determine whether the impaired mucus penetration was due to alterations in the sperm motility and, if so, whether these alterations were due to the SP or its viscosity, or to the medium components. To this end, artificial SP medium (ASP), a medium which supports motility but not capacitation, was compared with PBS and SP. Thawed, pooled semen from seven mature rams was layered under 1 ml each of PBS, SP and ASP and motile spermatozoa allowed to swim up (37 8C, 30 min). Upper regions of the overlays were harvested, and the capacitation status of the spermatozoa in each suspension determined by chlortetracycline (CTC) analysis. Sperm movement was videotaped in 300 mm chambers for both computer-aided sperm analysis assessment and manual flagellar curvature analysis. There was no effect of the culture medium on the concentration of spermatozoa recovered by swim up, nor on the proportion of motile spermatozoa. However, the spermatozoa resuspended in PBS did show changes associated with capacitation in both the CTC-binding patterns and in their movement patterns. These changes were significantly greater than those observed in spermatozoa resuspended in SP or ASP. These results indicated that the differences in sperm movement and function observed in SP medium were not due to changes in viscosity, but rather to components of the medium.

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“…Asthenospermia, also known as poor sperm motility, is one of the important factors for male infertility (Aitken et al, 1982a). Sperm cannot travel through a mucus-filled cervix to reach the site of fertilization if the sperm has low motility (Mortimer et al, 1986;Folgerø et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Investigation of polymorphisms in exon7 of the NSUN7 gene among Chinese Han men with asthenospermia

Hy¹,

Zhong²,

Xp³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Previous studies have shown that genetic polymorphisms in exon7 of the NSUN7 gene can be used as an infertility marker in Iranian men with asthenospermia. However, there have been no equivalent studies in China. In the present study, we investigated the possible association between the genetic polymorphisms in exon7 of NSUN7 and asthenospermia in a Chinese Han population. We recruited 240 asthenospermic men as a patient group and 256 normospermic men as a control group, and analyzed the semen parameters on the basis of World Health Organization (WHO) guidelines. The genetic polymorphisms in exon7 of NSUN7 were detected by DNA sequence analysis. The results were analyzed statistically and a P value < 0.05 was considered significant. There were two genetic polymorphisms, c.906C>T and c.922T>G, in exon7 of NSUN7. We found relatively similar genotypes and allele frequencies between the two groups (P = 0.928, P = 0.928, (2015) respectively). The combined genotypes of the two polymorphisms did not identify a haplotype associated with asthenospermia (P = 0.824, P = 0.824, respectively). Our findings revealed that genetic polymorphisms in exon7 of the NSUN7 gene are not associated with asthenospermia in Chinese Han men.

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Relationship between human sperm motility characteristics and sperm penetration into human cervical mucus in vitro

Cited by 136 publications

References 22 publications

The usefulness and significance of assessing rapidly progressive spermatozoa

The usefulness and significance of assessing rapidly progressive spermatozoa

Effect of medium on the kinematics of frozen–thawed ram spermatozoa

Investigation of polymorphisms in exon7 of the NSUN7 gene among Chinese Han men with asthenospermia

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