Human Sperm Motility, Viability, and Morphology Decreased after Cryopreservation

Abstract

The aim of this study was to analyze human sperm motility, viability, and morphology before and after cryopreservation. This true laboratory experimental study had pre and post randomized one group design. The study was conducted at the Embryology, Andrology, and Genetics Laboratory, Department of Medical Biology, Faculty of Medicine, Universitas Airlangga from August to November 2017. The eighteen samples of fresh semen were collected from male volunteers who agreed and signed the informed consent of the study. Samples were analyzed their motility, viability, and morphology before and after cryopreservation. Results of this study indicated differentiation between motility before and after cryopreservation. Cryopreservation process decreased progressive motility (42.22 + 9.46%; 17.83 + 6.24%; p< 0.0001) and increased the number of immotile spermatozoa (35.44 + 10.15%; 60.11 + 12.53%; p< 0.0001). Cryopreservation also decreased human sperm viability (73.78 + 8.91%; 40.83 + 12.89%; p< 0.0001) and morphology (10.94 + 4.96%; 7.39 + 3.90%; p< 0.0001). Cryopreservation of human spermatozoa caused the decreased of motility, viability, and morphology.

Sperm Cryopreservation: Principles and Biology

“…The inevitable change involves the significant reduction in motility mostly due to membrane changes and acrosome degeneration among the others. A decrease in spermatozoa kinetics after thawing has been reported consistently in many studies [26][27][28][29].…”

“…Another parameter affected by freezing is sperm morphology which mostly results due to the deformation in membrane structure due to dysregulated liquid influx [2,7,27,29]. It has also been shown that freeze preservation of sperm is followed by ultrastructural changes [11].…”

Effect of Carbohydrate Type and Phenotype on the Quality of Post-Thawing Frozen Semen of KUB Chicken

“…There are two types of cryoprotectants: extracellular and intracellular. Extracellular cryoprotectants protect the cell membrane from the osmotic pressure created by freezing; one of these agents is sugar (Darsini et al 2019). Osmotic stress can cause structural changes in sperm, altering sperm morphology (Yeste et al 2017).…”

Effects of Titanium Dioxide Nanoparticles and Coenzyme Q10 on Testicular Ischemia-Reperfusion Injury: Role of the Mitochondrial Apoptosis Pathway

“…The produced sperm are transferred to another tube, called the epididymis, where they are stored [2]. Male infertility depends on various parameters, such as the number, motility, viability, and morphology of the sperms, which are affected by several factors [3]. There are several reasons for insufficient blood supply to testicular tissue, which causes hypoxia and oxidative stress [4].…”