Sperm Cryopreservation: Principles and Biology

doi:10.47277/jirb/8(3)/43

Cited by 7 publications

(3 citation statements)

References 58 publications

(95 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Freezing can cause changes in sperm morphology. These changes are caused by changes in the ultrastructural of sperm due to drastic temperature changes, osmotic pressure, and the formation of crystal ice in sperm (Sharma & Sharma 2020).…”

Section: )mentioning

confidence: 99%

Effect of Carbohydrate Type and Phenotype on the Quality of Post-Thawing Frozen Semen of KUB Chicken

Maghfira,

Karja,

Arifiantini

et al. 2023

JITV

View full text Add to dashboard Cite

The superior Balitbangtan Kampung Chicken (KUB) chickens have different phenotypes. It was reported that the chicken phenotype was related to semen quality. This study aimed to determine the post-thawing characteristics and quality of KUB chicken semen with different phenotypes frozen in Ringer's lactate egg yolk (RLEY) diluent with the addition of fructose or glucose. Semen was collected using the massaging method from 20 KUB chickens with a single comb phenotype and black or dark brown feather color with a red feather neck (SCNR), green-black single comb with white feather neck collar (SCNW), pea comb and black feathers or dark brown fur with a red neck (PCNR), and a green-black pea comb with a white neck (PCNW). Semen from each chicken phenotype was divided into three parts or frozen in three types of diluents: RLEY, RLEY+fructose (RLEYF), and RLEY+glucose (RLEYG). The highest sperm motility was found in the diluent with the addition of glucose in the SCNR and PCNW phenotypes (P0.05). The highest sperm viability was shown in the RLEYG diluent in the PCNW phenotype (P0.05). The highest abnormality was found in the RLEY and RLEYF diluents in the SCNW, PCNR, and PCNW groups, whereas in the RLEYG group, it was only found in the PCNR group. From the results of this study, it can be concluded that the type of glucose and chicken phenotype influences the quality of post-thawing semen. The best is found in diluents with glucose attachments in the SCNR phenotype.

show abstract

Section: )mentioning

confidence: 99%

Effect of Carbohydrate Type and Phenotype on the Quality of Post-Thawing Frozen Semen of KUB Chicken

Maghfira,

Karja,

Arifiantini

et al. 2023

JITV

View full text Add to dashboard Cite

show abstract

“…Early attempts for sperm vitality conservation may be traced back to 1776, when Spallanzani froze spermatozoa using snow and subsequently demonstrated their motility after rewarming [ 1 ]. Since then, remarkable progress in the evolution of techniques and media for short- or long-term semen storage has allowed sperm preservation to overcome numerous space and time limitations, and thus to become an integral part of assisted reproduction technologies (ARTs) in animals and humans [ 1 , 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…Sperm preservation, at low temperatures and when properly performed, enables the long-term storage of male gametes in a state of metabolic arrest that prevents cellular senescence while maintaining their viability and fertilizing potential, therefore this allows them to be used when and where they are needed [ 2 ]. While the role of sperm storage at low temperatures as a critical pillar of reproductive technologies is undoubtable, the rationale behind its use for the management of male fertility may differ between animal and human ARTs [ 3 , 4 , 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Strategies for Bacterial Eradication from Human and Animal Semen Samples: Current Options and Future Alternatives

Ďuračka

Benko

Chňapek

et al. 2023

Sensors

View full text Add to dashboard Cite

The primary role of semen processing and preservation is to maintain a high proportion of structurally and functionally competent and mature spermatozoa, that may be used for the purposes of artificial reproduction when needed, whilst minimizing any potential causes of sperm deterioration during ex vivo semen handling. Out of a multitude of variables determining the success of sperm preservation, bacterial contamination has been acknowledged with an increased interest because of its often unpredictable and complex effects on semen quality. Whilst antibiotics are usually the most straight-forward option to prevent the bacterial contamination of semen, antimicrobial resistance has become a serious threat requiring widespread attention. As such, besides discussing the consequences of bacteriospermia on the sperm vitality and the risks of antibiotic overuse in andrology, this paper summarizes the currently available evidence on alternative strategies to prevent bacterial contamination of semen prior to, during, and following sperm processing, selection, and preservation. Alternative antibacterial supplements are reviewed, and emphasis is given to modern methods of sperm selection that may be combined by the physical removal of bacteria prior to sperm preservation or by use in assisted reproductive technologies.

show abstract

Tissue and sperm handling before assisted reproductive technology (ART): A systematic review

Ambar

Gava

Ghirelli-Filho

et al. 2021

Arab Journal of Urology

View full text Add to dashboard Cite

Objective: : To explore the medical literature on techniques of tissue and sperm handling after surgical retrieval for intracytoplasmic sperm injection (ICSI). Methods: : A search was performed in PubMed and Google Scholar databases, according to a modified Preferred Reporting Items for Systemic Reviews and Meta-Analyses (PRISMA) guideline, considering the studies investigating tissue handling and sperm selection techniques for ICSI. Results: : Overall, 42 articles were included in this study, investigating sample handling, methods for sperm selection, and the use of chemical compounds to improve sperm motility and fertilisation rates. Conclusion: :The ideal sperm handling method should provide a high sperm count, high vitality and appropriate sperm function, without side-effects. In this review the most common and useful techniques are described and the best combination strategies discussed in clinical scenarios.

show abstract

Sperm Cryopreservation: Principles and Biology

Cited by 7 publications

References 58 publications

Effect of Carbohydrate Type and Phenotype on the Quality of Post-Thawing Frozen Semen of KUB Chicken

Effect of Carbohydrate Type and Phenotype on the Quality of Post-Thawing Frozen Semen of KUB Chicken

Strategies for Bacterial Eradication from Human and Animal Semen Samples: Current Options and Future Alternatives

Tissue and sperm handling before assisted reproductive technology (ART): A systematic review

Contact Info

Product

Resources

About