Human Sperm Binding Is Mediated by the Sialyl-Lewis
            <sup>x</sup>
            Oligosaccharide on the Zona Pellucida

Pang, Poh-Choo; Chiu, Philip C.N.; Lee, Cheuk‐Lun; Chang, Lan-Yi; Panico, Maria; Morris, Howard R.; Haslam, Stuart M.; Khoo, Kay‐Hooi; Clark, Gary F.; Yeung, William S.B.; Dell, Anne

doi:10.1126/science.1207438

Cited by 284 publications

(241 citation statements)

References 33 publications

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“…The ability to block FUTs using small molecule inhibitors to alter cell surface glycosylation, in a dose-and time-dependent manner, within different models should speed recognition and understanding of the biological roles played by this epitope (20). Small molecule inhibitors will also present the opportunity to validate FUTs as an attractive therapeutic target given the roles played by fucosylated glycoconjugates such as sLe X in mediating processes like tumor metastasis and chronic inflammation.…”

Section: Resultsmentioning

confidence: 99%

“…Because Fuc is generally present as a terminal sugar residue within glycans, including sLe X , these ␣1,3-specific FUTs are of interest because their blockade may be less generally disruptive than targeting early biosynthetic steps. Small molecule inhibitors would be useful probes to propel our understanding of the functional role of FUTs in diverse biological processes including, for example, fertilization (20) and cell surface receptor signaling (21). Several lines of evidence also support ␣1,3-specific FUTs as promising targets for therapeutic intervention.…”

mentioning

confidence: 99%

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Metabolic Inhibition of Sialyl-Lewis X Biosynthesis by 5-Thiofucose Remodels the Cell Surface and Impairs Selectin-Mediated Cell Adhesion

Zandberg

Jayakanthan

Pinto

et al. 2012

Journal of Biological Chemistry

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Background: Sialyl-Lewis X (sLe X ) is a fucosylated oligosaccharide that plays critical roles in cell adhesion. Results: 5-thiofucose is metabolized by cells to produce a new nucleotide sugar that impairs sLe X biosynthesis and cell adhesiveness. Conclusion: 5-thiofucose blocks fucose transfer within treated cells. Significance: 5-thiofucose should be useful in further elucidating the biological roles of sLe X .

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Metabolic Inhibition of Sialyl-Lewis X Biosynthesis by 5-Thiofucose Remodels the Cell Surface and Impairs Selectin-Mediated Cell Adhesion

Zandberg

Jayakanthan

Pinto

et al. 2012

Journal of Biological Chemistry

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“…Boar SP includes a large number of glycoproteins, which also includes the aforementioned spermadhesins. Their facility for glycosylation gives them an important role in sperm functionality [34], particularly in regulating sperm motility, capacitation, acrosome reaction and sperm-oocyte binding [35]. Some identified SP-proteins show ion-binding properties, being particularly relevant for sperm performance those showing zinc-and calcium-binding properties.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the porcine seminal plasma proteome comparing ejaculate portions

Pérez-Patiño

Barranco

Parrilla

et al. 2016

Journal of Proteomics

102

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Full identification of SP-proteins remains challenging, particularly in some livestock species such as porcine. This experimental study aims to provide an extensive proteomic analysis of boar SP and to generate a public accessible database of boar SP-proteome. A SP-pool from 33 entire ejaculates from 11 boars (3 ejaculates per boar) was analyzed to characterize the boar SP-proteome. Moreover, 20 ejaculates collected in fractions (P1: first 10 mL of sperm rich ejaculate fraction (SRF), P2: rest of SRF and P3: post-SRF) from 5 boars (4 ejaculates per boar) were analyzed to evaluate differentially expressed SP-proteins among portions. SP-samples were subjected to a combination of SEC, 1-D SDS PAGE and NanoLC-ESI-MS/MS followed by functional bioinformatics analysis.The identified proteins were quantified from normalized LFQ intensity data. A total of 33,557 spectra corresponding to 8,189 peptides and 536 SP-proteins were identified with ≥ 95% Confidence (Unused Score > 1.3) and a false discovery rate (FDR) ≤ 1%. Of the 536 SP-proteins, 409 were identified in Sus Scrofa taxonomy and 374 of them were Biological Significance: This proteomic study provides the major characterization of the boar SP-proteome with more than 250 proteins first reported. The boar SP-proteome is described so that a spectral library can be built for relative 'label free' protein quantitation with SWATH approach. This proteomic profiling allows the creation of a publicly accessible database of the boar SP-proteome, as a first step for further understanding the role of SP-proteins in reproductive outcomes as well as for identification of biomarkers for sperm quality and fertility.

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“…It consists of 3 parts including the acrosome inner membrane, outer acrosome membrane, and acrosome antrum. When an acrosome reaction occurs, acrosome enzymes are released to promote acrosome inner membrane exposure, and then the specific molecules on the acrosome inner membrane participate in zone pellucida identification of egg cells [12].…”

Section: Discussionmentioning

confidence: 99%

Value Analysis of Sperm Spontaneous Acrosome Reaction in Male Fertility Evaluation

T¹,

W²,

Xie³

et al. 2017

Andrology

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Objective: Using correlation analysis between the sperm spontaneous acrosome reaction rate (SARR) and routine semen parameters in male infertility patients, we explored the value of the sperm spontaneous acrosome reaction in the evaluation of sperm functions. Methods:The participants comprised of 219 male infertility patients who had visited the Center for Reproductive Medicine, Shandong University from October 2016 to March 2017. According to the sperm SARR, we classified the patients with infertility into the control group and the case group. According to the WHO human semen examination and processing laboratory manual, fifth edition, recommendations and standards. We obtained routine semen parameters including semen volume, sperm concentration, total motility, and progressive sperm rate, and we measured sperm motility rate and the proportion of sperm with normal morphology.Results: With regard to the total motility, progressive sperm rate, sperm survival rate and the proportion of sperm with normal morphology, there were statistically significant differences between the two groups (p<0.01), but the data concerning patients' age, semen volume, or sperm concentration showed no significant difference (p>0.05). Conclusion:Sperm SARR in patients with infertility is closely related to routine semen parameters and plays a supplementary reference in male fertility evaluation.

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Human Sperm Binding Is Mediated by the Sialyl-Lewis ^x Oligosaccharide on the Zona Pellucida

Abstract: Fertilization in humans is initiated by binding of spermatozoa to a selectin ligand on the egg’s extracellular matrix.

Cited by 284 publications

References 33 publications

Metabolic Inhibition of Sialyl-Lewis X Biosynthesis by 5-Thiofucose Remodels the Cell Surface and Impairs Selectin-Mediated Cell Adhesion

Metabolic Inhibition of Sialyl-Lewis X Biosynthesis by 5-Thiofucose Remodels the Cell Surface and Impairs Selectin-Mediated Cell Adhesion

Characterization of the porcine seminal plasma proteome comparing ejaculate portions

Value Analysis of Sperm Spontaneous Acrosome Reaction in Male Fertility Evaluation

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Human Sperm Binding Is Mediated by the Sialyl-Lewis x Oligosaccharide on the Zona Pellucida

Abstract: Fertilization in humans is initiated by binding of spermatozoa to a selectin ligand on the egg’s extracellular matrix.

Cited by 284 publications

References 33 publications

Metabolic Inhibition of Sialyl-Lewis X Biosynthesis by 5-Thiofucose Remodels the Cell Surface and Impairs Selectin-Mediated Cell Adhesion

Metabolic Inhibition of Sialyl-Lewis X Biosynthesis by 5-Thiofucose Remodels the Cell Surface and Impairs Selectin-Mediated Cell Adhesion

Characterization of the porcine seminal plasma proteome comparing ejaculate portions

Value Analysis of Sperm Spontaneous Acrosome Reaction in Male Fertility Evaluation

Contact Info

Product

Resources

About

Human Sperm Binding Is Mediated by the Sialyl-Lewis ^x Oligosaccharide on the Zona Pellucida