Value Analysis of Sperm Spontaneous Acrosome Reaction in Male Fertility Evaluation

T, Li; W, Liu; Xie, Ning; Sj, Yang; C, Zhang; Hl, Fu; Gao, Xin

doi:10.4172/2167-0250.1000195

Cited by 2 publications

(2 citation statements)

References 16 publications

(13 reference statements)

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“…This speculation was confirmed by the spermatozoa acrosome integrity test, as 17.80% of the spermatozoa in the LSM were acrosome damaged. Similar to the previous report in humans (59), the results here suggest that acrosome degradation might be one of the factors leading to poor sperm motility.…”

Section: Discussionsupporting

confidence: 91%

Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens

Li¹,

Sun²,

Ni³

et al. 2020

Molecular & Cellular Proteomics

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Molecular mechanisms underlying sperm motility have not been fully explained, particularly in chickens. The objective was to identify seminal plasma proteins associated with chicken sperm motility by comparing the seminal plasma proteomic profile of roosters with low sperm motility (LSM, n = 4) and high sperm motility (HSM, n = 4). Using a label-free MS-based method, a total of 522 seminal plasma proteins were identified, including 386 (∼74%) previously reported and 136 novel ones. A total of 70 differentially abundant proteins were defined, including 48 more-abundant, 15 less-abundant, and seven proteins unique to the LSM group (specific proteins). Key secretory proteins like less-abundant adhesion G-protein coupled receptor G2 (ADGRG2) and more-abundant serine peptidase inhibitor Kazal-type 2 (SPINK2) in the LSM suggested that the corresponding secretory tissues played a crucial role in maintaining sperm motility. Majority (80%) of the more-abundant and five specific proteins were annotated to the cytoplasmic domain which might be a result of higher plasma membrane damage and acrosome dysfunction in LSM. Additionally, more-abundant mitochondrial proteins were detected in LSM seminal plasma associated with lower spermatozoa mitochondrial membrane potential (ΔΨm) and ATP concentrations. Further studies showed that the spermatozoa might be suffering from oxidative stress, as the amount of spermatozoa reactive oxygen species (ROS) were largely enhanced, seminal malondialdehyde (MDA) concentrations were increased, and the seminal plasma total antioxidant capacity (T-AOC) were decreased. Our study provides an additional catalogue of chicken seminal plasma proteome and supports the idea that seminal plasma could be as an indicator of spermatozoa physiology. More-abundant of acrosome, mitochondria and sperm cytoskeleton proteins in the seminal plasma could be a marker of sperm dysfunction and loss of motility. The degeneration of spermatozoa caused by the reduced seminal T-AOC and enhanced oxidative stress might be potential determinants of low sperm motility. These results could extend our understanding of sperm motility and sperm physiology regulation.

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Section: Discussionsupporting

confidence: 91%

Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens

Li¹,

Sun²,

Ni³

et al. 2020

Molecular & Cellular Proteomics

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“…For the acrosome reaction to occur, it is essential that sperm membrane integrity is intact and metabolic processes within spermatozoa structures run in a normal way [Šerniené et al 2005]. Spermatozoa with premature acrosome reaction are incapable of fusion with egg [Li et al 2017]. Cell membrane of boar spermatozoa is particularly sensitive to temperature changes due to its specific structure.…”

Section: Introductionmentioning

confidence: 99%

The occurrence of spermatozoa with acrosome reaction in semen of boars depending on staining method and storage duration

Wysokińs¹,

Chłopik²

2020

Acta Sci. Pol. Zootech.

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The aim of the present study was to analyze the frequency of occurrence of acrosome-reacted spermatozoa in semen stained with two methods, depending on boar semen storage time. The studies were conducted on ejaculates collected from 10 Landrace boars used in artificial insemination. The smears were prepared by means of two staining methods: SpermBlue and eosin-gentian dye. It was concluded that the proportion of acrosome-reacted spermatozoa depends on the staining method and ejaculate storage duration. When the smears were stained with the SpermBlue method, the number of spermatozoa with head defects, including acrosome reaction, was greater compared with the smears stained with the eosin-gentian dye. With the passage of semen storage time, a change in sperm count with an acrosome reaction was observed. In the SpermBlue-stained smears, the proportion of acrosome-reacted spermatozoa was the highest in the 96th hour of storage and was about 2.5%. An increase in the number of acrosome-reacted spermatozoa was observed in the semen stained with the eosin-gentian dye method in the following semen storage hours.

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Value Analysis of Sperm Spontaneous Acrosome Reaction in Male Fertility Evaluation

Cited by 2 publications

References 16 publications

Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens

Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens

The occurrence of spermatozoa with acrosome reaction in semen of boars depending on staining method and storage duration

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