Human small intestinal and colonic tissue mounted in the Ussing chamber as a tool for characterizing the intestinal absorption of drugs

Rozehnal, Veronika; Nakai, Daisuke; Hoepner, Ursula; Fischer, Thomas M.; Kamiyama, Emi; Takahashi, Masayuki; Yasuda, Satoru; Mueller, Juergen

doi:10.1016/j.ejps.2012.02.025

Cited by 124 publications

(92 citation statements)

References 36 publications

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“…Previous studies by Haslam et al (2011), Rozehnal et al (2012 and Sjöberg et al (2013) have shown that the intestinal tissue remains viable only for up to 150 min (on average 120 min), which is long enough to study absorption and/or secretion of satiety hormones. For the investigation of the impact of food bioactives on other health effects 2 h may be too short for any clear response, such as release of cytokines, reversible disruption of tight junctions, extensive metabolism.…”

Section: The Ussing Chambermentioning

confidence: 99%

“…As the Ussing chamber technique uses intestinal tissue segments, they still contain the morphological and physiological features of the intestine, including interplay of many complex processes, such as interaction between the multicellular environment (Rozehnal et al 2012). Using ex vivo intestinal tissue segments gives a better representation of the complex in vivo morphology (multicellular conglomeration, presence of the mucus layer) and thereby a better representation of the various possible processes involved in the in vivo situation.…”

Section: The Ussing Chambermentioning

confidence: 99%

“…Other markers that can be studied in parallel to a typical transport study include the intestinal metabolism of testosterone and midazolam, by measuring the presence of metabolites apically, basolaterally and intracellularly by means of, for instance, High Pressure Liquid Chromatography (HPLC) (Sjöberg et al 2013). Transport via the paracellular pathway can be assessed by measuring the permeability of Lucifer yellow (Papp value <6 × 10 −6 cm/s) (Rozehnal et al 2012) leakage of fluorescein isothiocyanate dextran to the basolateral side (FD4; <0.5 %) , or the linearity of transport of atenolol (R 2 > 0.995 with at least 3 datapoints) (Haslam et al 2011). Values higher than the ones mentioned above, indicate leakage of the intestinal tissue.…”

Section: Monitoring Viabilitymentioning

confidence: 99%

“…Previous studies by Haslam et al (2011), Rozehnal et al (2012 and Sjöberg et al (2013) have used the potential difference (>4 mV), short-circuit current (>100 μA/cm 2 ), trans epithelial electrical resistance (TEER; >20 Ω cm 2 ) for continuous monitoring of the viability. Low resistance indicates tissue leakage.…”

Section: Monitoring Viabilitymentioning

confidence: 99%

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Ussing Chamber

Westerhout¹,

Wortelboer²,

Verhoeckx³

2015

The Impact of Food Bioactives on Health

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The Ussing chamber system is named after the Danish zoologist Hans Ussing, who invented the device in the 1950s to measure the short-circuit current as an indicator of net ion transport taking place across frog skin (Ussing and Zerahn, Acta Physiol Scand 23:110-127, 1951). Ussing chambers are increasingly being used to measure ion transport in native tissue, like gut mucosa, and in a monolayer of cells grown on permeable supports. However, the Ussing chamber system is, to date, not often applied for the investigation of the impact of food bioactives (proteins, sugars, lipids) on health.An Ussing system is generally comprised of a chamber and a perfusion system, and if needed, an amplifier and data acquisition system. The heart of the system lies in the chamber with the other components performing supporting roles. The classic chamber design is still in wide use today. However, several newer designs are now available that optimize for convenience and for diffusion-or electrophysiologybased measurements. A well designed Ussing chamber supports an epithelia membrane or cell monolayer in such a way that each side of the membrane is isolated and faces a separate chamber-half. The chambers are then filled with a physiologically relevant solution, such as Ringer's solution. This configuration allows the researcher to make unique chemical and electrical adjustments to either side of the membrane with complete control. The Ussing chamber technique has its strengths and limitations, which will be explained in more detail in this chapter.

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Section: The Ussing Chambermentioning

confidence: 99%

Section: The Ussing Chambermentioning

confidence: 99%

Section: Monitoring Viabilitymentioning

confidence: 99%

Section: Monitoring Viabilitymentioning

confidence: 99%

See 2 more Smart Citations

Ussing Chamber

Westerhout¹,

Wortelboer²,

Verhoeckx³

2015

The Impact of Food Bioactives on Health

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“…The ussing technique is an accepted procedure for the use of tissues and cell cultures for transport studies (Bajka et al, 2003;Rozehnal et al, 2012;Song et al, 2013). The native porcine intestinal epithelium is an established model for studies of the transport of ions and high molecular weight substances (Herrmann et al, 2012;Miyake et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Development of in vitro screening system for food habit related risk analysis

Bruch

Möhr²

2015

JEBAS

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In order to assess the health risk that associated with the consumption of unknown feed or food ingredients, there is a strong need of developing an in vitro screening system. The test system should be fast, reliable, inexpensive and without the necessity of performing animal tests. Furthermore, it should also provide important clues to the potential danger of unknown substances. The present study examines the extent to which cell and tissue cultures can be used for such studies. It should be ascertained whether the cell cultures can replace the native intestinal epithelium in terms of their sensitivity and provide accurate results as a quick "screening system". As a model for intestinal operations ex vivo tissue cultures from the native intestinal epithelium of the pig and the permanent cell line IPEC-J2 were used. The cell culture was characterized in terms of their morphological and functional properties (TEER, tight-junction proteins).Various studies (short-circuit measurements, translocation of [ 3 H]-mannitol) were performed to IPEC-J2 cells and the native intestinal epithelium in order to compare the functional properties of both systems. Finally, the response of the addition of "unknown" test substances (papain and wheat extract) were investigated to determine whether the functional parameters of both systems are affected by these test substances or not. The IPEC-J2 cells show a more significant influence in their functionality by "unknown" substances than the control variant. Results of study revealed that the in vitro system reacts rapidly in response of unknown test substances and it is more sensitive. Therefore, it is possible to operate a "risk assessment" for "unknown" substances with the help of this developed screening system.

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Drug Transporters in the Human Intestine

Oswald¹

2022

Drug Transporters

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Human small intestinal and colonic tissue mounted in the Ussing chamber as a tool for characterizing the intestinal absorption of drugs

Cited by 124 publications

References 36 publications

Ussing Chamber

Ussing Chamber

Development of in vitro screening system for food habit related risk analysis

Drug Transporters in the Human Intestine

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