Human skin permeation rates ex vivo following exposures to mixtures of glycol ethers

Berthet, Aurélie; Oltramare, Christelle; Spring, Philipp; Hechon, Julie; Hopf, Nancy B.

doi:10.1016/j.toxlet.2020.09.017

Cited by 5 publications

(4 citation statements)

References 30 publications

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“…The removed skin was then cleaned to remove any remaining fat and rinsed with PBS at pH 7.4 to finish. For later usage, the cleansed skin was wrapped in aluminum foil and stored in a deep freezer at −30 °C (Al-Mahallawi et al., 2019 ; Berthet et al., 2020 ). A methylene blue dye test was done to check the skin integrity prior to completing the in vitro skin permeation investigations (Berthet et al., 2020 ).…”

Section: Methodsmentioning

confidence: 99%

“…For later usage, the cleansed skin was wrapped in aluminum foil and stored in a deep freezer at −30 °C (Al-Mahallawi et al., 2019 ; Berthet et al., 2020 ). A methylene blue dye test was done to check the skin integrity prior to completing the in vitro skin permeation investigations (Berthet et al., 2020 ). After that, the Franz diffusion cells were put to an in vitro skin permeation test (Perme Gear, Inc., Hellertown, PA).…”

Section: Methodsmentioning

confidence: 99%

“…After that, the Franz diffusion cells were put to an in vitro skin permeation test (Perme Gear, Inc., Hellertown, PA). Furthermore, the excised skin was attached between the donor and receptor compartments, covering a 2 cm 2 effective surface area with a sink capacity of 20 mL (Berthet et al., 2020 ). The receptor compartment, on the other hand, was filled with ethanol as a permeation medium to keep the sink condition and the temperature at 37 ± 0.5 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Apart from this, CRT solution (containing 1 mg) was applied onto the skin of donor compartment for the comparison purpose. Furthermore, the applied formulation was evenly dispersed with the flat end of a spatula to keep the formulation intact with the skin for a longer period of time (Berthet et al., 2020 ). To maintain the receptor compartment volume, an aliquot of 2 mL of sample was withdrawn through the sampling port a set time period (0–24 h) and replaced with an equivalent amount of sink medium (Berthet et al., 2020 ).…”

Section: Methodsmentioning

confidence: 99%

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Crotamiton-loaded tea tree oil containing phospholipid-based microemulsion hydrogel for scabies treatment: in vitro , in vivo evaluation, and dermatokinetic studies

et al. 2021

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Crotamiton (CRT) is a commonly approved drug prescribed for the scabies treatment in many countries across the globe. However, poor aqueous solubility and low bioavailability, and side effects restrict its use. To avoid such issues, an appropriate carrier system is necessary which can address the aforementioned challenges for attaining enhanced biopharmaceutical attributes. The current study intends to provide a detailed account on the development and evaluation of CRT-loaded microemulsion (ME) hydrogel formulation containing tea tree oil (TTO) for improved drug delivery for scabies treatment in a safe and effective manner. Pseudo-ternary phase diagrams were constructed with TTO as the oily phase, and Cremophor ® EL was used as the surfactant in a mass ratio 2:1 with co-surfactants (mixture of phospholipid 90G and Transcutol ® P), and aqueous solution as the external phase. The optimized drug-loaded ME formulation was evaluated for skin penetration, retention, compliance, and dermatokinetics. The nonirritant behavior of the formulation was revealed by skin histopathology, which showed no changes in normal skin histology. In comparison to the conventional product, dermatokinetic experiments revealed that CRT has greater penetration and distribution in the epidermis of the mice skin. The findings imply that the proposed lipid-based ME hydrogel can aid in the resolution of CRT issues by providing a better and safer delivery option to epidermis and deeper epidermis in substantial quantities.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Crotamiton-loaded tea tree oil containing phospholipid-based microemulsion hydrogel for scabies treatment: in vitro , in vivo evaluation, and dermatokinetic studies

et al. 2021

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Enhanced skin localization of metronidazole using solid lipid microparticles incorporated into polymeric hydrogels for potential improved of rosacea treatment: An ex vivo proof of concept investigation

Sulistiawati

Dwipayanti

Azhar

et al. 2022

International Journal of Pharmaceutics

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An update of skin permeability data based on a systematic review of recent research

Chedik,

Baybekov,

Cosnier

et al. 2024

Sci Data

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The cutaneous absorption parameters of xenobiotics are crucial for the development of drugs and cosmetics, as well as for assessing environmental and occupational chemical risks. Despite the great variability in the design of experimental conditions due to uncertain international guidelines, datasets like HuskinDB have been created to report skin absorption endpoints. This review updates available skin permeability data by rigorously compiling research published between 2012 and 2021. Inclusion and exclusion criteria have been selected to build the most harmonized and reusable dataset possible. The Generative Topographic Mapping method was applied to the present dataset and compared to HuskinDB to monitor the progress in skin permeability research and locate chemotypes of particular concern. The open-source dataset (SkinPiX) includes steady-state flux, maximum flux, lag time and permeability coefficient results for the substances tested, as well as relevant information on experimental parameters that can impact the data. It can be used to extract subsets of data for comparisons and to build predictive models.

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Human skin permeation rates ex vivo following exposures to mixtures of glycol ethers

Cited by 5 publications

References 30 publications

Crotamiton-loaded tea tree oil containing phospholipid-based microemulsion hydrogel for scabies treatment: in vitro , in vivo evaluation, and dermatokinetic studies

Crotamiton-loaded tea tree oil containing phospholipid-based microemulsion hydrogel for scabies treatment: in vitro , in vivo evaluation, and dermatokinetic studies

Enhanced skin localization of metronidazole using solid lipid microparticles incorporated into polymeric hydrogels for potential improved of rosacea treatment: An ex vivo proof of concept investigation

An update of skin permeability data based on a systematic review of recent research

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